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Mouse Anti-AAV-2 VP1 Recombinant Antibody (V2-503413) (CBMAB-V208-1401-FY)

This product is mouse antibody that recognizes AAV-2 VP1. The antibody CBMW-H1229 can be used for immunoassay techniques such as: ELISA, IF, IHC, IP, WB.
See all AAV-2 VP1 antibodies
Published Data
Mouse Anti-AAV-2 VP1 Recombinant Antibody (CBMW-H1229) (CBMAB-V208-1401-FY) in Dot Blot
Immuno-dot blot analysis of native rAAV capsids of the panel of AAV serotypes 1-12. ...View More
Mietzsch, M., Grasse, S., Zurawski, C., Weger, S., Bennett, A., Agbandje-McKenna, M., ... & Heilbronn, R. (2014). OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Human gene therapy, 25(3), 212-222.

Summary

Host Animal
Mouse
Specificity
AAV
Clone
V2-503413
Antibody Isotype
IgG2a
Application
ELISA, IF, IHC, IP, WB

Basic Information

Immunogen
Adeno-associated virus capsid proteins and virus particles
Specificity
AAV
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adeno-Associated Virus-2 Capsid Protein Vp1
Introduction
Adeno-associated virus (AAV) is a small virus which infects humans and some other primate species. AAV is not currently known to cause disease.
Entrez Gene ID
4192015
UniProt ID
P03135
Alternative Names
VP1; Capsid protein VP1; AAV-2 VP1; Adeno-associated virus-2
Function
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus.
Biological Process
Clathrin-dependent endocytosis of virus by host cell
Permeabilization of host organelle membrane involved in viral entry into host cell
Viral entry via permeabilization of inner membrane
Virion attachment to host cell
Cellular Location
Virion; Host nucleolus. Capid proteins are first observed in the host nucleolus where capsid assembly may occur, and then are present over the whole nucleoplasm where encapsidation of the viral DNA takes place.

Han, I. C., Cheng, J. L., Burnight, E. R., Ralston, C. L., Fick, J. L., Thomsen, G. J., ... & Wiley, L. A. (2020). Retinal tropism and transduction of adeno-associated virus varies by serotype and route of delivery (intravitreal, subretinal, or suprachoroidal) in rats. Human Gene Therapy, 31(23-24), 1288-1299.

Earley, L. F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-associated virus serotype-specific inverted terminal repeat sequence role in vector transgene expression. Human gene therapy, 31(3-4), 151-162.

Kruzik, A., Fetahagic, D., Hartlieb, B., Dorn, S., Koppensteiner, H., Horling, F. M., ... & de la Rosa, M. (2019). Prevalence of anti-adeno-associated virus immune responses in international cohorts of healthy donors. Molecular Therapy-Methods & Clinical Development, 14, 126-133.

Mary, B., Maurya, S., Arumugam, S., Kumar, V., & Jayandharan, G. R. (2019). Post‐translational modifications in capsid proteins of recombinant adeno‐associated virus (AAV) 1‐rh10 serotypes. The FEBS journal, 286(24), 4964-4981.

Brandon-Warner, E., Benbow, J. H., Swet, J. H., Feilen, N. A., Culberson, C. R., McKillop, I. H., ... & Schrum, L. W. (2018). Adeno-Associated Virus Serotype 2 Vector–Mediated Reintroduction of microRNA-19b Attenuates Hepatic Fibrosis. Human gene therapy, 29(6), 674-686.

Wiley, L. A., Burnight, E. R., Kaalberg, E. E., Jiao, C., Riker, M. J., Halder, J. A., ... & Mullins, R. F. (2018). Assessment of adeno-associated virus serotype tropism in human retinal explants. Human gene therapy, 29(4), 424-436.

Lee, E. J., Guenther, C. M., & Suh, J. (2018). Adeno-associated virus (AAV) vectors: Rational design strategies for capsid engineering. Current opinion in biomedical engineering, 7, 58-63.

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For research use only. Not intended for any clinical use.

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