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Mouse Anti-AIM2 Recombinant Antibody (V2-180355) (CBMAB-A1815-YC)

Provided herein is a Mouse monoclonal antibody against Human Absent In Melanoma 2. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AIM2 antibodies
Published Data
Mouse Anti-AIM2 Recombinant Antibody (B-8) in WB and IF
AIM2 was expressed in brain endothelial cells, and its expression increased after ischemic stroke. A, Western blot images of AIM2 and GAPDH expression on days 1, 3, and 7 after MCAO. B, Quantification of AIM2 expression normalized to that of GAPDH. C, Image showing the location at which immunostaining was conducted in the penumbra of the infarcted brain. D, Immunofluorescence images of DAPI (blue)/AIM2 (green)/CD31 (red) colocalization on days 1 and 3 after MCAO. Scale bar: 20 μm. E, Percentage of the CD31+AIM2+ vascular length with respect to the total CD31+ vascular length. F, Western blot images of AIM2 and GAPDH expression after OGD/R. G, Quantification of AIM2 expression normalized to that of GAPDH. H, AIM2 mRNA expression after OGD/R. All data are presented as the mean ±SEM. *p < 0.05 and ** p < 0.01 compared with the control group. ...View More
Xu, S. Y., Bian, H. J., Shu, S., Xia, S. N., Gu, Y., Zhang, M. J., ... & Cao, X. (2021). AIM2 deletion enhances blood-brain barrier integrity in experimental ischemic stroke. CNS Neuroscience & Therapeutics, 27(10), 1224-1237.
Mouse Anti-AIM2 Recombinant Antibody (B-8) in WB
SOD3 inhibited P. acnes- or PGN-induced inflammasome in HaCaT and SZ95 cells. (A) HaCaT and SZ95 (B) cells were pretreated with SOD3 (200 U/mL) and incubated with heat-killed P. acnes (100 MOI) or peptidoglycan (10 μg/mL) for 24 h. Expression of inflammasome-related proteins was analyzed by Western blot. ...View More
Nguyen, C. T., Sah, S. K., Zouboulis, C. C., & Kim, T. Y. (2018). Inhibitory effects of superoxide dismutase 3 on Propionibacterium acnes-induced skin inflammation. Scientific Reports, 8(1), 4024.
Mouse Anti-AIM2 Recombinant Antibody (B-8) in IHC
Over-expression of AIM2 inhibits renal carcinoma cell migration and invasion in vitro. (A-D). Cell migration assay was performed after the over-expression of AIM2 in 786-O and OSRC-2 cells. (E and H). Matrigel cell
invasion assay was performed after the over-expression of AIM2 in 786-O and OSRC-2 cells. Data are from one representative experiment of three performed and presented as the mean ±SD. The different significance was set at *p < 0.05, **p < 0.01, and ***p < 0.001. ...View More
Chai, D., Shan, H., Wang, G., Li, H., Fang, L., Song, J., ... & Zheng, J. (2018). AIM2 is a potential therapeutic target in human renal carcinoma and suppresses its invasion and metastasis via enhancing autophagy induction. Experimental cell research, 370(2), 561-570.
Mouse Anti-AIM2 Recombinant Antibody (B-8) in WB
HEK293T cells were transfected with constructs coexpressing the indicated full-length (wt) form of IFI16, PYHIN1, MNDA and AIM2 or just the N-terminal PYD or PYD and linker region and BFP or a vector control and expression was analyzed by Western blot. BFP and GAPDH are used as transfection and loading control, respectively. ...View More
Bosso, M., Prelli Bozzo, C., Hotter, D., Volcic, M., Stürzel, C. M., Rammelt, A., ... & Kirchhoff, F. (2020). Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells. PLoS pathogens, 16(8), e1008752.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180355
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 10-179 mapping near the N-terminus of AIM2 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Absent In Melanoma 2
Introduction
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
Entrez Gene ID
9447
UniProt ID
O14862
Alternative Names
Absent In Melanoma 2; Interferon-Inducible Protein AIM2; PYHIN4;
Function
Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
Biological Process
Activation of innate immune response Source: UniProtKB
Apoptotic process Source: UniProtKB-KW
Cellular response to drug Source: MGI
Cellular response to interferon-beta Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
NLRP3 inflammasome complex assembly Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
Positive regulation of defense response to virus by host Source: UniProtKB
Positive regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Pyroptosis Source: UniProtKB
Pyroptosome complex assembly Source: GO_Central
Tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus. Activated inflammasomes can aggregate in the cytosol as speck-like particles.

Figueira, M. B. D. A., de Lima, D. S., Boechat, A. L., Antunes, I. A., Matsuda, J. D. S., Ribeiro, T. R. D. A., ... & Sadahiro, A. (2021). Single-Nucleotide Variants in the AIM2–Absent in Melanoma 2 Gene (rs1103577) Associated With Protection for Tuberculosis. Frontiers in immunology, 12, 824.

Li, Y. K., Chen, J. G., & Wang, F. (2021). The emerging roles of absent in melanoma 2 (AIM2) inflammasome in central nervous system disorders. Neurochemistry International, 105122.

Vakrakou, A. G., Svolaki, I. P., Evangelou, K., Gorgoulis, V. G., & Manoussakis, M. N. (2020). Cell-autonomous epithelial activation of AIM2 (absent in melanoma-2) inflammasome by cytoplasmic DNA accumulations in primary Sjögren's syndrome. Journal of autoimmunity, 108, 102381.

Yang, Y., Zhang, M., Jin, C., Ding, Y., Yang, M., Wang, R., ... & Hu, R. (2019). Absent in melanoma 2 suppresses epithelial‐mesenchymal transition via Akt and inflammasome pathways in human colorectal cancer cells. Journal of cellular biochemistry, 120(10), 17744-17756.

Zhu, W., Zu, X., Liu, S., & Zhang, H. (2019). The absent in melanoma 2 (AIM2) inflammasome in microbial infection. Clinica Chimica Acta, 495, 100-108.

Choubey, D. (2019). Type I interferon (IFN)-inducible Absent in Melanoma 2 proteins in neuroinflammation: implications for Alzheimer’s disease. Journal of neuroinflammation, 16(1), 1-10.

Shi, X., Wang, L., Ren, L., Li, J., Li, S., Cui, Q., & Li, S. (2019). Dihydroartemisinin, an antimalarial drug, induces absent in melanoma 2 inflammasome activation and autophagy in human hepatocellular carcinoma HepG2215 cells. Phytotherapy Research, 33(5), 1413-1425.

Zhang, Z., Dong, X., Yang, X., Wan, D., Sun, L., Gu, M., ... & He, S. (2017). Expression and clinical significance of absent in melanoma 2 in colorectal cancer. Biomedicine & Pharmacotherapy, 94, 843-849.

Choubey, D., & Panchanathan, R. (2017). Absent in Melanoma 2 proteins in SLE. Clinical immunology, 176, 42-48.

Sun, Q., Loughran, P., Shapiro, R., Shrivastava, I. H., Antoine, D. J., Li, T., ... & Scott, M. J. (2017). Retracted: Redox‐dependent regulation of hepatocyte absent in melanoma 2 inflammasome activation in sterile liver injury in mice. Hepatology, 65(1), 253-268.

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For research use only. Not intended for any clinical use.

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