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Mouse Anti-AKAP5 Recombinant Antibody (V2-180441) (CBMAB-A1929-YC)

Provided herein is a Mouse monoclonal antibody against Human A-Kinase Anchoring Protein 5. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AKAP5 antibodies
Published Data
Mouse Anti-AKAP5 Recombinant Antibody (CBYC-A479) in IP
Co-immunoprecipitation results for Flag-p85S6K or Flag-p70S6K with myc-GluA1, with AKAP79 co-transfected in HEK293 cells. ...View More
Li, J. B., Hu, X. Y., Chen, M. W., Xiong, C. H., Zhao, N., Ge, Y. H., ... & Qiu, Y. (2023). p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer's disease. Translational Neurodegeneration, 12(1), 1.
Mouse Anti-AKAP5 Recombinant Antibody (CBYC-A479) in IF
Immunofluorescence images showing GFP and AKAP79ΔPKA expression in the nucleus accumbens 3 days following HSV-AKAP79ΔPKA injection. Arrows highlight examples of colocalization between GFP and AKAP79ΔPKA. ...View More
Guercio, L. A., Hofmann, M. E., Swinford-Jackson, S. E., Sigman, J. S., Wimmer, M. E., Dell'Acqua, M. L., ... & Pierce, R. C. (2018). A-kinase anchoring protein 150 (AKAP150) promotes cocaine reinstatement by increasing AMPA receptor transmission in the accumbens shell. Neuropsychopharmacology, 43(6), 1395-1404.
Mouse Anti-AKAP5 Recombinant Antibody (CBYC-A479) in IHC
Expression of AKAP5 in lung tissue from patients with COPD and control subjects. AKAP5 protein expression was analyzed by immunohistochemistry. A representative image from a control patient's lung is shown (A). Bars indicate 100 μm. B: semiquantification of expression in the ASM of COPD vs. control patients. See Table 2 for details of the patients; all individuals were ex-smokers. White arrows indicate ASM. Results are expressed as individual data points for each patient with the median of the separate experiments, *P < 0.05, control patients vs. patients with COPD using a Kruskal-Wallis 1-way ANOVA followed by Mann-Whitney U-test. ...View More
Poppinga, W. J., Heijink, I. H., Holtzer, L. J., Skroblin, P., Klussmann, E., Halayko, A. J., ... & Schmidt, M. (2015). A-kinase-anchoring proteins coordinate inflammatory responses to cigarette smoke in airway smooth muscle. American Journal of Physiology-Lung Cellular and Molecular Physiology, 308(8), L766-L775.
Mouse Anti-AKAP5 Recombinant Antibody (CBYC-A479) in WB
Decreased AKAP9 protein expression by CSE is prevented by st-Ht31. AKAP5 and AKAP12 expression was analyzed by Western blot and RT-PCR after stimulation with 1% CSE for 6 and 24 h, respectively. Representative immunoblots are shown. ...View More
Oldenburger, A., Poppinga, W. J., Kos, F., de Bruin, H. G., Rijks, W. F., Heijink, I. H., ... & Schmidt, M. (2014). A-kinase anchoring proteins contribute to loss of E-cadherin and bronchial epithelial barrier by cigarette smoke. American Journal of Physiology-Cell Physiology, 306(6), C585-C597.
Mouse Anti-AKAP5 Recombinant Antibody (CBYC-A479) in Immunoblot
Expression of proteins targeted with siRNAs was established by immunoblotting of the same blots. ...View More
Koçer, S. S., Wang, H. Y., & Malbon, C. C. (2012). "Shaping" of cell signaling via AKAP-tethered PDE4D: Probing with AKAR2-AKAP5 biosensor. Journal of Molecular Signaling, 7(1), 1-14.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180441
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Amino acids 181-285 of AKAP 79 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
A-Kinase Anchoring Protein 5
Introduction
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene
Entrez Gene ID
9495
UniProt ID
P24588
Alternative Names
A-Kinase Anchoring Protein 5; CAMP-Dependent Protein Kinase Regulatory Subunit II High Affinity-Binding Protein; A Kinase (PRKA) Anchor Protein 5; A-Kinase Anchor Protein 79 KDa; AKAP 79; AKAP79;
Function
Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors (PubMed:1512224). Association with the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Plays a role in long term synaptic potentiation by regulating protein trafficking from the dendritic recycling endosomes to the plasma membrane and controlling both structural and functional plasticity at excitatory synapses (PubMed:25589740).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Chemical synaptic transmission Source: ProtInc
Negative regulation of adenylate cyclase activity Source: UniProtKB
Positive regulation of adenylate cyclase activity Source: GO_Central
Positive regulation of endosome to plasma membrane protein transport Source: UniProtKB
Positive regulation of long-term synaptic potentiation Source: UniProtKB
Positive regulation of protein localization to plasma membrane Source: UniProtKB
Regulation of protein kinase A signaling Source: UniProtKB
Signal transduction Source: ProtInc
Cellular Location
Postsynaptic recycling endosome membrane; Lipid-anchor.Associates with lipid rafts.
PTM
Palmitoylated (PubMed:21771783, PubMed:25589740). Palmitoylation at Cys-36 and Cys-129 play a key role in the targeting of AKAP5 to lipid rafts (PubMed:21771783). Palmitoylation by ZDHHC2 is required for AKAP5 function in LTP-stimulated recycling endosome exocytosis (PubMed:25589740).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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