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Mouse Anti-ATP5IF1 Recombinant Antibody (A0655) (CBMAB-A0655-LY)

The product is antibody recognizes ATPIF1. The antibody 3E2 immunoassay techniques such as: WB, ELISA.
See all ATP5IF1 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human, Cattle, Mouse, Rat
Clone
A0655
Antibody Isotype
IgG2b
Application
WB, IHC, IF, ICC, FC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human ATPIF1 (AA: 1-106) expressed in E. coli.
Specificity
Human, Cattle, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
IF(ICC)1:100-1:500
ELISA1:10,000
IHC1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATP Synthase Inhibitory Factor Subunit 1
Introduction
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
ATPI; ATPIP; IP; MGC1167; MGC8898
Function
Endogenous F1F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F1F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F1F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Biological Process
Angiogenesis Source: UniProtKB
Erythrocyte differentiation Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Heme biosynthetic process Source: UniProtKB
Mitochondrial depolarization Source: ParkinsonsUK-UCL
Negative regulation of ATPase activity Source: ParkinsonsUK-UCL
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of hydrolase activity Source: UniProtKB
Positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway Source: Ensembl
Positive regulation of proteolysis involved in cellular protein catabolic process Source: ParkinsonsUK-UCL
Reactive oxygen species metabolic process Source: Ensembl
Regulation of ATP metabolic process Source: ParkinsonsUK-UCL
Regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Cellular Location
Mitochondrion
PTM
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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