IHC-P, IF, IHC, WB
Alpha tubulin, Tetrahymena thermophila and Tetrahymena pyriformis (mixture)
Algae, C. elegans, Dictyostelium, Common fruit fly, Fish, Crab, Human, Medusa, Mouse, Protist, Yeast, Tetrahymena, Xenopus
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Tubulin alpha chain; Alpha-tubulin