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Mouse Anti-C4A Recombinant Antibody (CBFYC-0639) (CBMAB-C0693-FY)

This product is mouse antibody that recognizes C4A. The antibody CBFYC-0639 can be used for immunoassay techniques such as: ELISA, WB.
See all C4A antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-0639
Antibody Isotype
IgG1, k
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Complement C4A
Introduction
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway.
Entrez Gene ID
UniProt ID
Alternative Names
C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
Function
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Biological Process
Cellular protein metabolic process Source: Reactome
Complement activation Source: BHF-UCL
Complement activation, classical pathway Source: UniProtKB-KW
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Positive regulation of apoptotic cell clearance Source: BHF-UCL
Post-translational protein modification Source: Reactome
Regulation of complement activation Source: Reactome
Cellular Location
Secreted; Synapse; Axon; Dendrite
Involvement in disease
Complement component 4A deficiency (C4AD): A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
Systemic lupus erythematosus (SLE): Disease susceptibility is associated with variants affecting the gene represented in this entry. Interindividual copy-number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.
PTM
Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Yilmaz, M., Yalcin, E., Presumey, J., Aw, E., Ma, M., Whelan, C. W., ... & Carroll, M. C. (2021). Overexpression of schizophrenia susceptibility factor human complement C4A promotes excessive synaptic loss and behavioral changes in mice. Nature neuroscience, 24(2), 214-224.

Han, X., de la Fuente, M., & Nieman, M. T. (2021). Complement factor C4a does not activate protease‐activated receptor 1 (PAR1) or PAR4 on human platelets. Research and Practice in Thrombosis and Haemostasis, 5(1), 104-110.

Simoni, L., Presumey, J., van der Poel, C. E., Castrillon, C., Chang, S. E., Utz, P. J., & Carroll, M. C. (2020). Complement C4A regulates autoreactive B cells in murine lupus. Cell reports, 33(5), 108330.

Morales, R., Zhang, L., Wu, Y., Wilcox, J., Engelman, T., Ni, Y., ... & Tang, W. W. (2020). C3a, C4a, and C5a Complement Anaphylatoxins Are Paradoxically Decreased in Acute and Chronic Heart Failure. Journal of Cardiac Failure, 26(10), S19-S20.

Jüptner, M., Flachsbart, F., Caliebe, A., Lieb, W., Schreiber, S., Zeuner, R., ... & Schröder, J. O. (2018). Low copy numbers of complement C4 and homozygous deficiency of C4A may predispose to severe disease and earlier disease onset in patients with systemic lupus erythematosus. Lupus, 27(4), 600-609.

Liesmaa, I., Paakkanen, R., Järvinen, A., Valtonen, V., & Lokki, M. L. (2018). Clinical features of patients with homozygous complement C4A or C4B deficiency. PloS one, 13(6), e0199305.

Wang, H., Ricklin, D., & Lambris, J. D. (2017). Complement-activation fragment C4a mediates effector functions by binding as untethered agonist to protease-activated receptors 1 and 4. Proceedings of the National Academy of Sciences, 114(41), 10948-10953.

Zorzetto, M., Datturi, F., Divizia, L., Pistono, C., Campo, I., Silvestri, A. D., ... & Ricevuti, G. (2017). Complement C4A and C4B gene copy number study in Alzheimer's disease patients. Current Alzheimer Research, 14(3), 303-308.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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