Sign in or Register   Sign in or Register
  |  

Rat Anti-C5 Recombinant Antibody (295108) (CBMAB-C0801-LY)

This product is antibody recognizes C5. The antibody 295108 immunoassay techniques such as: WB, Neutralization, ELISA (Cap).
See all C5 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
295108
Antibody Isotype
IgG2a
Application
WB, Neutralization, ELISA (Cap)

Basic Information

Immunogen
E. coli-derived recombinant mouse Complement Component C5a, Asn679-Arg755, Accession # P06684
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
complement C5
Entrez Gene ID
UniProt ID
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049).
C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Biological Process
Activation of MAPK activity Source: ProtInc
Cell surface receptor signaling pathway Source: ProtInc
Chemotaxis Source: ProtInc
Complement activation Source: Reactome
Complement activation, alternative pathway Source: UniProtKB-KW
Complement activation, classical pathway Source: UniProtKB-KW
Cytolysis Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: Reactome
Inflammatory response Source: ProtInc
In utero embryonic development Source: Ensembl
Negative regulation of macrophage chemotaxis Source: BHF-UCL
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of chemokine production Source: BHF-UCL
Positive regulation of vascular endothelial growth factor production Source: BHF-UCL
Regulation of complement activation Source: Reactome
Cellular Location
Secreted
Involvement in disease
Complement component 5 deficiency (C5D): A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele.

Ariceta, G., Dixon, B. P., Kim, S. H., Kapur, G., Mauch, T., Ortiz, S., ... & Han, K. H. (2021). The long-acting C5 inhibitor, ravulizumab, is effective and safe in pediatric patients with atypical hemolytic uremic syndrome naïve to complement inhibitor treatment. Kidney International, 100(1), 225-237.

Laurence, J., Mulvey, J. J., Seshadri, M., Racanelli, A., Harp, J., Schenck, E. J., ... & Magro, C. M. (2020). Anti-complement C5 therapy with eculizumab in three cases of critical COVID-19. Clinical Immunology, 219, 108555.

de Latour, R. P., Bergeron, A., Lengline, E., Dupont, T., Marchal, A., Galicier, L., ... & Fremeaux-Bacchi, V. (2020). Complement C5 inhibition in patients with COVID-19-a promising target?. Haematologica, 105(12), 2847.

Mastellos, D. C., da Silva, B. G. P., Fonseca, B. A., Fonseca, N. P., Auxiliadora-Martins, M., Mastaglio, S., ... & Lambris, J. D. (2020). Complement C3 vs C5 inhibition in severe COVID-19: Early clinical findings reveal differential biological efficacy. Clinical Immunology, 220, 108598.

Röth, A., Nishimura, J. I., Nagy, Z., Gaàl-Weisinger, J., Panse, J., Yoon, S. S., ... & Peffault de Latour, R. (2020). The complement C5 inhibitor crovalimab in paroxysmal nocturnal hemoglobinuria. Blood, 135(12), 912-920.

Martínez-López, D., Roldan-Montero, R., García-Marqués, F., Nuñez, E., Jorge, I., Camafeita, E., ... & Martin-Ventura, J. L. (2020). Complement C5 protein as a marker of subclinical atherosclerosis. Journal of the American College of Cardiology, 75(16), 1926-1941.

Reichhardt, M. P., Johnson, S., Tang, T., Morgan, T., Tebeka, N., Popitsch, N., ... & Lea, S. M. (2020). An inhibitor of complement C5 provides structural insights into activation. Proceedings of the National Academy of Sciences, 117(1), 362-370.

Jendza, K., Kato, M., Salcius, M., Srinivas, H., De Erkenez, A., Nguyen, A., ... & Michaud, G. A. (2019). A small-molecule inhibitor of C5 complement protein. Nature chemical biology, 15(7), 666-668.

Fukuzawa, T., Sampei, Z., Haraya, K., Ruike, Y., Shida-Kawazoe, M., Shimizu, Y., ... & Nezu, J. (2017). Long lasting neutralization of C5 by SKY59, a novel recycling antibody, is a potential therapy for complement-mediated diseases. Scientific reports, 7(1), 1-12.

Keshari, R. S., Silasi, R., Popescu, N. I., Patel, M. M., Chaaban, H., Lupu, C., ... & Lupu, F. (2017). Inhibition of complement C5 protects against organ failure and reduces mortality in a baboon model of Escherichia coli sepsis. Proceedings of the National Academy of Sciences, 114(31), E6390-E6399.

Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Rat Anti-C5 Recombinant Antibody (295108)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare