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Mouse Anti-CARM1 Recombinant Antibody (CAP304) (CBMAB-AP781LY)

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CAP304
Antibody Isotype
IgG
Application
IP, WB

Basic Information

Immunogen
Synthetic peptide, corresponding to the C-terminus of Human CARM1
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Coactivator Associated Arginine Methyltransferase 1
Introduction
CARM1 (Coactivator Associated Arginine Methyltransferase 1) is a Protein Coding gene. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and Organelle biogenesis and maintenance. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transcription regulatory region DNA binding. An important paralog of this gene is PRMT3.
Entrez Gene ID
UniProt ID
Alternative Names
Coactivator Associated Arginine Methyltransferase 1; Protein Arginine N-Methyltransferase 4; PRMT4; Coactivator-Associated Arginine Methyltransferase 1; Histone-Arginine Methyltransferase CARM1; EC 2.1.1.319; EC 2.1.1;
Function
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
Biological Process
Aging Source: Ensembl
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
Histone H3-R17 methylation Source: UniProtKB
Histone H3-R2 methylation Source: UniProtKB
Histone methylation Source: UniProtKB
Negative regulation of dendrite development Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of fat cell differentiation Source: UniProtKB
Regulation of intracellular estrogen receptor signaling pathway Source: UniProtKB
Regulation of lipid metabolic process Source: Reactome
Regulation of mRNA binding Source: Ensembl
Regulation of transcription, DNA-templated Source: UniProtKB
Response to cAMP Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Nucleus. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
PTM
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity).
Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.

Suresh, S., Huard, S., & Dubois, T. (2021). CARM1/PRMT4: Making Its Mark beyond Its Function as a Transcriptional Coactivator. Trends in Cell Biology.

Zhang, S., Wang, W., Wu, X., Liu, W., & Ding, F. (2020). miR‐16‐5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator‐associated arginine methyltransferase 1. Pathology international, 70(1), 12-20.

Porta, M., Amione, C., Barutta, F., Fornengo, P., Merlo, S., Gruden, G., ... & Trento, M. (2019). The co-activator-associated arginine methyltransferase 1 (CARM1) gene is overexpressed in type 2 diabetes. Endocrine, 63(2), 284-292.

Nie, M., Wang, Y., Guo, C., Li, X., Wang, Y., Deng, Y., ... & Zhao, Q. (2018). CARM1-mediated methylation of protein arginine methyltransferase 5 represses human γ-globin gene expression in erythroleukemia cells. Journal of Biological Chemistry, 293(45), 17454-17463.

Behera, A. K., Bhattacharya, A., Vasudevan, M., & Kundu, T. K. (2018). p53 mediated regulation of coactivator associated arginine methyltransferase 1 (CARM1) expression is critical for suppression of adipogenesis. The FEBS journal, 285(9), 1730-1744.

Mo, K., Xu, H., Gong, H., Lei, H., Wang, Y., Guo, W., ... & Tu, W. (2018). Dorsal root ganglia coactivator-associated arginine methyltransferase 1 contributes to peripheral nerve injury-induced pain hypersensitivities. Neuroscience, 394, 232-242.

Bang, J. I., Lee, E. H., Lee, A. R., Lee, J. I., Choi, S. H., Seol, D. W., ... & Lee, D. R. (2018). The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development. Scientific reports, 8(1), 1-9.

Lim, C. S., & Alkon, D. L. (2017). Inhibition of coactivator-associated arginine methyltransferase 1 modulates dendritic arborization and spine maturation of cultured hippocampal neurons. Journal of Biological Chemistry, 292(15), 6402-6413.

van Haren, M. J., Marechal, N., Troffer-Charlier, N., Cianciulli, A., Sbardella, G., Cavarelli, J., & Martin, N. I. (2017). Transition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1. Proceedings of the National Academy of Sciences, 114(14), 3625-3630.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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