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Rabbit Anti-CASP3 Recombinant Antibody (E61) (CBMAB-P0137-YC)

Provided herein is a Rabbit monoclonal antibody against Human Caspase 3. The antibody can be used for immunoassay techniques, such as IHC-P.
See all CASP3 antibodies
Published Data

Summary

Host Animal
Rabbit
Specificity
Human
Clone
E61
Antibody Isotype
IgG
Application
IHC-P

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Caspase 3
Introduction
CASP3 is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Entrez Gene ID
UniProt ID
Alternative Names
A830040C14Rik; AC-3; Apopain; CASP-3; CC3; CPP-32; CPP32; Caspase-3; Lice; SCA-1; Yama; mldy
Function
Involved in the activation cascade of caspases responsible for apoptosis execution (PubMed:7596430).
At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond (PubMed:7774019).
Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (PubMed:7596430).
Involved in the cleavage of huntingtin (PubMed:8696339).
Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690).
Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800).
Biological Process
Anterior neural tube closure Source: Ensembl
Apoptotic DNA fragmentation Source: Reactome
Apoptotic process Source: UniProtKB
Apoptotic signaling pathway Source: BHF-UCL
Axonal fasciculation Source: Ensembl
B cell homeostasis Source: Ensembl
Cell fate commitment Source: Ensembl
Cellular response to DNA damage stimulus Source: Ensembl
Cellular response to staurosporine Source: CAFA
Cytokine-mediated signaling pathway Source: Reactome
Erythrocyte differentiation Source: UniProtKB
Execution phase of apoptosis Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
Glial cell apoptotic process Source: Ensembl
Heart development Source: Ensembl
Hippocampus development Source: Ensembl
Hippo signaling Source: Reactome
Intrinsic apoptotic signaling pathway in response to osmotic stress Source: Ensembl
Keratinocyte differentiation Source: GO_Central
Learning or memory Source: Ensembl
Leukocyte apoptotic process Source: Ensembl
Luteolysis Source: Ensembl
Negative regulation of activated T cell proliferation Source: Ensembl
Negative regulation of apoptotic process Source: MGI
Negative regulation of B cell proliferation Source: Ensembl
Neuron apoptotic process Source: Ensembl
Neuron differentiation Source: GO_Central
Neurotrophin TRK receptor signaling pathway Source: MGI
Platelet formation Source: UniProtKB
Positive regulation of amyloid-beta formation Source: UniProtKB
Positive regulation of apoptotic process Source: GO_Central
Positive regulation of neuron apoptotic process Source: Ensembl
Protein processing Source: Ensembl
Proteolysis Source: UniProtKB
Regulation of macroautophagy Source: ParkinsonsUK-UCL
Regulation of protein stability Source: UniProtKB
Response to amino acid Source: Ensembl
Response to antibiotic Source: Ensembl
Response to cobalt ion Source: Ensembl
Response to drug Source: Ensembl
Response to estradiol Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to glucose Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to hypoxia Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to nicotine Source: Ensembl
Response to tumor necrosis factor Source: BHF-UCL
Response to UV Source: Ensembl
Response to X-ray Source: Ensembl
Sensory perception of sound Source: Ensembl
Striated muscle cell differentiation Source: Ensembl
T cell homeostasis Source: Ensembl
Wound healing Source: Ensembl
Cellular Location
Cytoplasm
PTM
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Yildiz Gulhan, P., Eroz, R., Ataoglu, O., İnce, N., Davran, F., Öztürk, C. E., ... & Balbay, O. A. (2021). The evaluation of both the expression and serum protein levels of Caspase‐3 gene in patients with different degrees of SARS‐CoV2 infection. Journal of Medical Virology.

Awazu, M., Yamaguchi, Y., Nagata, M., Miura, M., & Hida, M. (2021). Caspase-3 regulates ureteric branching in mice via cell migration. Biochemical and Biophysical Research Communications, 559, 28-34.

Yousefi, M., Koopaie, M., Karimi, R., Kermani, F. M., Kolahdooz, S., & Shamshiri, A. (2021). Effect of photodynamic therapy on expression of HRAS, NRAS and caspase 3 genes at mRNA levels, apoptosis of head and neck squamous cell carcinoma cell line. Photodiagnosis and Photodynamic Therapy, 33, 102142.

Wang, Y. J., Liu, M. G., Wang, J. H., Cao, W., Wu, C., Wang, Z. Y., ... & Li, X. Y. (2020). Restoration of cingulate long-term depression by enhancing non-apoptotic caspase 3 alleviates peripheral pain hypersensitivity. Cell Reports, 33(6), 108369.

Suzuki, T., Ichii, O., Nakamura, T., Horino, T., Elewa, Y. H. A., & Kon, Y. (2020). Immune-associated renal disease found in caspase 3-deficient mice. Cell and tissue research, 379(2), 323-335.

Hentzen, N. B., Mogaki, R., Otake, S., Okuro, K., & Aida, T. (2020). Intracellular Photoactivation of Caspase-3 by Molecular Glues for Spatiotemporal Apoptosis Induction. Journal of the American Chemical Society, 142(18), 8080-8084.

Han, R., Chen, X., Li, Y., Zhang, S., Li, R., & Lu, L. (2019). MicroRNA-34a suppresses aggressiveness of hepatocellular carcinoma by modulating E2F1, E2F3, and Caspase-3. Cancer management and research, 11, 2963.

Bernard, A., Chevrier, S., Beltjens, F., Dosset, M., Viltard, E., Lagrange, A., ... & Boidot, R. (2019). Cleaved caspase-3 transcriptionally regulates angiogenesis-promoting chemotherapy resistance. Cancer research, 79(23), 5958-5970.

Vera‐Lozada, G., Segges, P., Stefanoff, C. G., Barros, M. H. M., Niedobitek, G., & Hassan, R. (2018). Pathway‐focused gene expression profiles and immunohistochemistry detection identify contrasting association of caspase 3 (CASP3) expression with prognosis in pediatric classical Hodgkin lymphoma. Hematological oncology, 36(4), 663-670.

Zhou, M., Liu, X., Li, Z., Huang, Q., Li, F., & Li, C. Y. (2018). Caspase‐3 regulates the migration, invasion and metastasis of colon cancer cells. International journal of cancer, 143(4), 921-930.

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For research use only. Not intended for any clinical use.

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