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Mouse Anti-CBX4 Recombinant Antibody (CBLC-LY-004) (CBMAB-C9403-LY)

This product is antibody recognizes CBX4. The antibody CBLC-LY-004 immunoassay techniques such as: IP.
See all CBX4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBLC-LY-004
Antibody Isotype
IgG1
Application
IP

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.09% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Chromobox 4
Introduction
CBX4 (Chromobox 4) is a Protein Coding gene. Among its related pathways are SUMOylation and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include chromatin binding and ligase activity. An important paralog of this gene is CBX2.
Entrez Gene ID
8535
UniProt ID
O00257
Alternative Names
Chromobox 4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; PC2; Chromobox Homolog 4 (Pc Class Homolog, Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila);
Function
E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed:12679040).
Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed:22825850).
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:12167701, PubMed:19636380, PubMed:21282530).
PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:12167701, PubMed:19636380, PubMed:21282530).
Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (By similarity).
Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).
Biological Process
Chromatin organization Source: UniProtKB-KW
Negative regulation of apoptotic process Source: ProtInc
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Cellular Location
Nucleus; Nucleus speckle. Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880). Localized in distinct foci on chromatin (PubMed:18927235).
PTM
Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.

Wang, Z., Fang, Z., Chen, G., Liu, B., Xu, J., Li, F., ... & Ji, H. (2021). Chromobox 4 facilitates tumorigenesis of lung adenocarcinoma through the Wnt/β-catenin pathway. Neoplasia, 23(2), 222-233.

Yang, Z., OuYang, X., Zheng, L., Dai, L., & Luo, W. (2021). Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis. Bioengineered, 12(1), 1012-1025.

Wen, L. J., Wang, Y. S., & Tan, P. Y. (2021). miR‑515‑5p inhibits the proliferation, migration and invasion of human breast cancer cells by targeting CBX4. Experimental and Therapeutic Medicine, 22(5), 1-9.

Zhao, J., Yang, T., & Li, L. (2020). LncRNA FOXP4-AS1 is involved in cervical cancer progression via regulating miR-136-5p/CBX4 axis. OncoTargets and therapy, 13, 2347.

Wang, X., Qin, G., Liang, X., Wang, W., Wang, Z., Liao, D., ... & Kang, T. (2020). Targeting the CK1α/CBX4 axis for metastasis in osteosarcoma. Nature communications, 11(1), 1-14.

Hu, C., Zhang, Q., Tang, Q., Zhou, H., Liu, W., Huang, J., ... & Zhang, R. (2020). CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. Journal of cellular and molecular medicine, 24(1), 618-631.

Huang, F. J., Dang, J. Q., Zhang, S., & Cheng, Z. Y. (2020). Circular RNA hsa_circ_0008039 promotes proliferation, migration and invasion of breast cancer cells through upregulating CBX4 via sponging miR-515-5p. Eur Rev Med Pharmacol Sci, 24(4), 1887-1898.

Zeng, J. S., Zhang, Z. D., Pei, L., Bai, Z. Z., Yang, Y., Yang, H., & Tian, Q. H. (2018). CBX4 exhibits oncogenic activities in breast cancer via Notch1 signaling. The international journal of biochemistry & cell biology, 95, 1-8.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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