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Mouse Anti-CD163 Recombinant Antibody (CBYY-C1196) (CBMAB-C2633-YY)

This product is mouse antibody that recognizes CD163. The antibody CBYY-C1196 can be used for immunoassay techniques such as: IF, WB
See all CD163 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C1196
Antibody Isotype
IgG1
Application
IF, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein A/G purified
Buffer
1 mg/mL
Preservative
50% glycerol, 1% BSA, PBS, pH 7.3
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD163 Molecule
Entrez Gene ID
UniProt ID
Alternative Names
CD2 Molecule; CD2 Antigen (P50), Sheep Red Blood Cell Receptor; T-Cell Surface Antigen T11/Leu-5; Erythrocyte Receptor; Rosette Receptor; LFA-3 Receptor; LFA-2;
Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Biological Process
Acute-phase response Source: UniProtKB-KW
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Receptor-mediated endocytosis Source: Reactome
Cellular Location
Secreted; Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
Topology
Extracellular: 42-1050
Helical: 1051-1071
Cytoplasmic: 1072-1156
PTM
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.

Huang, C., Bernard, D., Zhu, J., Dash, R. C., Chu, A., Knupp, A., ... & Tang, Y. (2020). Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells. Virology journal, 17(1), 1-11.

Zhang, Y., Zhang, K., Zheng, H., Liu, C., Jiang, Y., Du, N., ... & Gao, F. (2020). Development of a monoclonal antibody against porcine CD163 SRCR5 domain which partially blocks infection of PRRSV. Frontiers in Veterinary Science, 7, 923.

Kowalska, W. (2020). Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients. Folia histochemica et cytobiologica, 58(1), 17-24.

Fujiwara, Y., Ohnishi, K., Horlad, H., Saito, Y., Shiraishi, D., Takeya, H., ... & Komohara, Y. (2020). CD163 deficiency facilitates lipopolysaccharide‐induced inflammatory responses and endotoxin shock in mice. Clinical & translational immunology, 9(9), e1162.

Nielsen, M. C., Hvidbjerg Gantzel, R., Clària, J., Trebicka, J., Møller, H. J., & Grønbæk, H. (2020). Macrophage activation markers, CD163 and CD206, in acute-on-chronic liver failure. Cells, 9(5), 1175.

Nunes, P. R., Romão-Veiga, M., Peraçoli, J. C., Costa, R. A. A., de Oliveira, L. G., Borges, V. T. M., & Peraçoli, M. T. (2019). Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia. Immunologic research, 67(2), 194-201.

Liu, S., Zhang, C., Maimela, N. R., Yang, L., Zhang, Z., Ping, Y., ... & Zhang, Y. (2019). Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma. Oncoimmunology, 8(7), e1601478.

Shiraishi, D., Fujiwara, Y., Horlad, H., Saito, Y., Iriki, T., Tsuboki, J., ... & Komohara, Y. (2018). CD163 is required for protumoral activation of macrophages in human and murine sarcoma. Cancer research, 78(12), 3255-3266.

Cheng, Z., Zhang, D., Gong, B., Wang, P., & Liu, F. (2017). CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer. Oncotarget, 8(50), 87244.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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