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Mouse Anti-CD33 (AA 337-364) Recombinant Antibody (574CT16.4.4) (CBMAB-C1111-YY)

This product is mouse antibody that recognizes CD33. The antibody 574CT16.4.4 can be used for immunoassay techniques such as: ELISA, WB, IF
See all CD33 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
574CT16.4.4
Antibody Isotype
IgM
Application
ELISA, WB, IF

Basic Information

Specificity
Human
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Euglobulin precipitation
Preservative
PBS
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 337-364

Target

Full Name
CD33 Molecule
Entrez Gene ID
945
UniProt ID
P20138
Alternative Names
Casein Kinase 1 Gamma 2; CKI-Gamma 2; EC 2.7.11.1; CK1g2; Casein Kinase 1 Isoform Gamma-2; Casein Kinase I Isoform Gamma-2; Casein Kinase 1, Gamma 2; Casein Kinase I; EC 2.7.11;
Function
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212).
Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872).
Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109).
These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109).
In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109).
One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
Biological Process
Cell adhesion Source: GO_Central
Cell-cell adhesion Source: ARUK-UCL
Cell-cell signaling Source: ProtInc
Immune response-inhibiting signal transduction Source: UniProtKB
Negative regulation of calcium ion transport Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of interleukin-1 beta production Source: ARUK-UCL
Negative regulation of interleukin-8 production Source: ARUK-UCL
Negative regulation of monocyte activation Source: ARUK-UCL
Negative regulation of tumor necrosis factor production Source: ARUK-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of protein secretion Source: UniProtKB
Positive regulation of protein tyrosine phosphatase activity Source: ARUK-UCL
Regulation of immune response Source: Reactome
Signal transduction Source: ProtInc
Cellular Location
Isoform CD33M: Cell membrane
Isoform CD33m: Peroxisome. CD33m isoform does not localize to cell surfaces but instead accumulates in peroxisomes.
Topology
Extracellular: 18-259
Helical: 260-282
Cytoplasmic: 283-364
PTM
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition.
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.

Marcinek, A., Brauchle, B., Hänel, G., Lacher, S. M., Zieger, N., Kischel, R., ... & Subklewe, M. (2021). CD33 BiTE® Construct Mediated Immunological Synapse Formation and Downstream Signaling in T Cells Is Dependent on Expression of Costimulatory Molecules on Target Cells. Blood, 138(Supplement 1), 2237-2237.

Chiu, E., Felices, M., Cichocki, F., Davis, Z., Wang, H., Tuninga, K., ... & Miller, J. S. (2021). Anti-NKG2C/IL-15/anti-CD33 killer engager directs primary and iPSC-derived NKG2C+ NK cells to target myeloid leukemia. Molecular Therapy.

Molica, M., Perrone, S., Mazzone, C., Niscola, P., Cesini, L., Abruzzese, E., & de Fabritiis, P. (2021). CD33 Expression and gentuzumab ozogamicin in acute myeloid leukemia: Two sides of the same coin. Cancers, 13(13), 3214.

Rotiroti, M. C., Buracchi, C., Arcangeli, S., Galimberti, S., Valsecchi, M. G., Perriello, V. M., ... & Tettamanti, S. (2020). Targeting CD33 in chemoresistant AML patient-derived xenografts by CAR-CIK cells modified with an improved SB transposon system. Molecular Therapy, 28(9), 1974-1986.

Griciuc, A., Federico, A. N., Natasan, J., Forte, A. M., McGinty, D., Nguyen, H., ... & Maguire, C. A. (2020). Gene therapy for Alzheimer’s disease targeting CD33 reduces amyloid beta accumulation and neuroinflammation. Human molecular genetics, 29(17), 2920-2935.

Miles, L. A., Hermans, S. J., Crespi, G. A., Gooi, J. H., Doughty, L., Nero, T. L., ... & Parker, M. W. (2019). Small molecule binding to Alzheimer risk factor CD33 promotes Aβ phagocytosis. Iscience, 19, 110-118.

Subklewe, M., Stein, A., Walter, R. B., Bhatia, R., Wei, A. H., Ritchie, D., ... & Ravandi, F. (2019). Preliminary results from a phase 1 first-in-human study of AMG 673, a novel half-life extended (HLE) anti-CD33/CD3 BiTE®(bispecific T-cell engager) in patients with relapsed/refractory (R/R) acute myeloid leukemia (AML).

Westervelt, P., Roboz, G. J., Cortes, J. E., Kantarjian, H. M., Lee, S., Rettig, M. P., ... & DiPersio, J. F. (2018). Phase 1 first-in-human trial of AMV564, a bivalent bispecific (2x2) CD33/CD3 T-cell Engager, in patients with relapsed/refractory acute myeloid leukemia (AML). Blood, 132, 1455.

Son, M., Diamond, B., Volpe, B. T., Aranow, C. B., Mackay, M. C., & Santiago-Schwarz, F. (2017). Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression. Scientific reports, 7(1), 1-13.

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For research use only. Not intended for any clinical use.

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