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Rabbit Anti-CD3E Recombinant Antibody (SP7) (CBMAB-C4100-CQ)

This product is a rabbit antibody that recognizes CD3E. The antibody SP7 can be used for immunoassay techniques such as: WB, FC, IHC-P.
See all CD3E antibodies
Published Data
Fig.1 Histologic phenotype of the Hupki livers following a high fat diet.
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Fig.2 Histologic analyses of Hu-PBL-NSG mouse model Lung, colon and kidney tissue stained with anti-CD3 antibody in Hu-PBL-NSG mouse (above) compared to human tissue.
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Fig.3 IHC assays for the levels of CD3, Gr1, TLR7, 120G8, IL-23p19 and IL-17 A were performed on day 8 skin (400×).
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Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
SP7
Antibody Isotype
IgG
Application
WB, FC, IHC-P

Basic Information

Immunogen
A synthetic peptide corresponding tointernal region of the epsilon chain of human CD3 epsilon protein
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Tissue culture supernatant
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD3e Molecule
Introduction
CD3E (CD3e Molecule) is a Protein Coding gene. Diseases associated with CD3E include Immunodeficiency 18 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta. Among its related pathways are G-protein signaling N-RAS regulation pathway and Toll-like receptor signaling pathway. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and signal transducer activity.
Entrez Gene ID
Human916
Mouse12501
UniProt ID
HumanP07766
MouseP22646
Alternative Names
CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3;
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Biological Process
Adaptive immune response Source: UniProtKB-KW
Apoptotic signaling pathway Source: Ensembl
Cell surface receptor signaling pathway Source: GO_Central
Cerebellum development Source: Ensembl
Dendrite development Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Negative regulation of gene expression Source: UniProtKB
Negative regulation of smoothened signaling pathway Source: Ensembl
Negative thymic T cell selection Source: Ensembl
Positive regulation of alpha-beta T cell proliferation Source: Ensembl
Positive regulation of calcium-mediated signaling Source: Ensembl
Positive regulation of cell-cell adhesion mediated by integrin Source: BHF-UCL
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of gene expression Source: UniProtKB
Positive regulation of interferon-gamma production Source: Ensembl
Positive regulation of interleukin-2 production Source: Ensembl
Positive regulation of interleukin-4 production Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Positive regulation of T cell anergy Source: Ensembl
Positive regulation of T cell proliferation Source: UniProtKB
Positive thymic T cell selection Source: GO_Central
Protein-containing complex assembly Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of immune response Source: Reactome
Response to nutrient Source: Ensembl
Signal complex assembly Source: ProtInc
T cell activation Source: UniProtKB
T cell costimulation Source: Ensembl
T cell receptor signaling pathway Source: Reactome
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Cellular Location
Cell membrane
Involvement in disease
Immunodeficiency 18 (IMD18): An autosomal recessive primary immunodeficiency characterized by onset in infancy or early childhood of recurrent infections. The severity is variable, encompassing both a mild immunodeficiency and severe combined immunodeficiency (SCID), resulting in early death without bone marrow transplantation in some patients. Immunologic work-up of the IMD18 SCID patients shows a T cell-negative, B cell-positive, natural killer (NK) cell-positive phenotype, whereas T-cell development is not impaired in the mild form of IMD18.
Topology
Extracellular: 23-126
Helical: 127-152
Cytoplasmic: 153-207
PTM
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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