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Mouse Anti-CD40LG Recombinant Antibody (CBXC-1811) (CBMAB-C4814-CQ)

This product is a mouse antibody that recognizes CD40LG. The antibody CBXC-1811 can be used for immunoassay techniques such as: FC.
See all CD40LG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-1811
Antibody Isotype
IgG2a
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CD40 Ligand
Introduction
CD40LG (CD40 Ligand) is a Protein Coding gene. Diseases associated with CD40LG include Immunodeficiency With Hyper-Igm, Type 1 and Cd40 Ligand Deficiency. Among its related pathways are PEDF Induced Signaling and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include cytokine activity and CD40 receptor binding.
Entrez Gene ID
UniProt ID
Alternative Names
CD40 Ligand; TNF-Related Activation Protein; Tumor Necrosis Factor (Ligand) Superfamily Member 5; T-B Cell-Activating Molecule; CD40 Antigen Ligand; T-Cell Antigen Gp39; TNFSF5; CD40-L; CD40L; TRAP; Tumor Necrosis Factor (Ligand) Superfamily, Member 5 (Hyper-IgM Syndrome);
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Biological Process
Activation of JUN kinase activity Source: UniProtKB
B cell differentiation Source: Ensembl
B cell proliferation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Inflammatory response Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Isotype switching Source: UniProtKB
Leukocyte cell-cell adhesion Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Platelet activation Source: UniProtKB
Positive regulation of endothelial cell apoptotic process Source: BHF-UCL
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-12 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of immunoglobulin production Source: Ensembl
T cell costimulation Source: UniProtKB
Tumor necrosis factor-mediated signaling pathway Source: Reactome
Cellular Location
Cell membrane; Cell surface
CD40 ligand, soluble form: Secreted. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Involvement in disease
Immunodeficiency with hyper-IgM, type 1 (HIGM1): Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Topology
Cytoplasmic: 1-22
Helical: 23-46
Extracellular: 47-261
PTM
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Fadul, C. E., Mao-Draayer, Y., Ryan, K. A., Noelle, R. J., Wishart, H. A., Channon, J. Y., ... & Kasper, L. H. (2021). Safety and Immune Effects of Blocking CD40 Ligand in Multiple Sclerosis. Neurology-Neuroimmunology Neuroinflammation, 8(6).

França, T. T., Barreiros, L. A., Al-Ramadi, B. K., Ochs, H. D., Cabral-Marques, O., & Condino-Neto, A. (2019). CD40 ligand deficiency: treatment strategies and novel therapeutic perspectives. Expert review of clinical immunology, 15(5), 529-540.

Ferrua, F., Galimberti, S., Courteille, V., Slatter, M. A., Booth, C., Moshous, D., ... & SCETIDE, P. (2019). Hematopoietic stem cell transplantation for CD40 ligand deficiency: results from an EBMT/ESID-IEWP-SCETIDE-PIDTC study. Journal of Allergy and Clinical Immunology, 143(6), 2238-2253.

Du, X., Tang, W., Chen, X., Zeng, T., Wang, Y., Chen, Z., ... & Zhao, X. (2019). Clinical, genetic and immunological characteristics of 40 Chinese patients with CD40 ligand deficiency. Scandinavian journal of immunology, 90(4), e12798.

Saliba, D. G., Céspedes-Donoso, P. F., Bálint, Š., Compeer, E. B., Korobchevskaya, K., Valvo, S., ... & Dustin, M. L. (2019). Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. Elife, 8, e47528.

Cabral-Marques, O., França, T. T., Al-Sbiei, A., Schimke, L. F., Khan, T. A., Feriotti, C., ... & Condino-Neto, A. (2018). CD40 ligand deficiency causes functional defects of peripheral neutrophils that are improved by exogenous IFN-γ. Journal of Allergy and Clinical Immunology, 142(5), 1571-1588.

Komlósi, Z. I., Kovács, N., van de Veen, W., Kirsch, A. I., Fahrner, H. B., Wawrzyniak, M., ... & Akdis, C. A. (2018). Human CD40 ligand–expressing type 3 innate lymphoid cells induce IL-10–producing immature transitional regulatory B cells. Journal of Allergy and Clinical Immunology, 142(1), 178-194.

França, T. T., Leite, L. F., Maximo, T. A., Lambert, C. G., Zurro, N. B., Forte, W. C., & Condino-Neto, A. (2018). A novel de novo mutation in the CD40 ligand gene in a patient with a mild X-linked hyper-IgM phenotype initially diagnosed as CVID: new aspects of old diseases. Frontiers in pediatrics, 6, 130.

Han, W., Jackson, D. A., Matissek, S. J., Misurelli, J. A., Neil, M. S., Sklavanitis, B., ... & Elsawa, S. F. (2017). Novel molecular mechanism of regulation of CD40 ligand by the transcription factor GLI2. The Journal of Immunology, 198(11), 4481-4489.

Cabral-Marques, O., Ramos, R. N., Schimke, L. F., Khan, T. A., Amaral, E. P., Bomfim, C. C. B., ... & Condino-Neto, A. (2017). Human CD40 ligand deficiency dysregulates the macrophage transcriptome causing functional defects that are improved by exogenous IFN-γ. Journal of Allergy and Clinical Immunology, 139(3), 900-912.

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For research use only. Not intended for any clinical use.

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