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Mouse Anti-CDC25C Recombinant Antibody (CBFYC-1513) (CBMAB-C1572-FY)

This product is mouse antibody that recognizes CDC25C. The antibody CBFYC-1513 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all CDC25C antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1513
Antibody Isotype
IgG1
Application
ELISA, IHC, WB

Basic Information

Immunogen
Purified recombinant fragment of human CDC25C expressed in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cell Division Cycle 25C
Introduction
CDC25C (Cell Division Cycle 25C) is a Protein Coding gene. Diseases associated with CDC25C include Plague and Prostate Cancer. Among its related pathways are Gene Expression and Akt Signaling. Gene Ontology (GO) annotations related to this gene include protein kinase binding and WW domain binding. An important paralog of this gene is CDC25B.
Entrez Gene ID
995
UniProt ID
P30307
Alternative Names
Cell Division Cycle 25C; Protein Phosphatase 1, Regulatory Subunit 60; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C
Function
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Biological Process
Cell division Source: UniProtKB-KW
Cell population proliferation Source: ProtInc
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
G2/M transition of mitotic cell cycle Source: UniProtKB
Positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
Positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Regulation of mitotic nuclear division Source: ProtInc
Spermatogenesis Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus
PTM
Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 oCcurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Liu, K., Zheng, M., Lu, R., Du, J., Zhao, Q., Li, Z., ... & Zhang, S. (2020). The role of CDC25C in cell cycle regulation and clinical cancer therapy: a systematic review. Cancer Cell International, 20, 1-16.

Galusic, D., Lucijanic, M., Livun, A., Radman, M., Lucijanic, J., Hofman, I. D., & Kusec, R. (2020). CDC25c expression in patients with myelofibrosis is associated with stronger myeloproliferation and shorter overall survival. Wiener klinische Wochenschrift, 1-3.

Xun, R., Lu, H., & Wang, X. (2020). Identification of CDC25C as a Potential Biomarker in Hepatocellular Carcinoma Using Bioinformatics Analysis. Technology in Cancer Research & Treatment, 19, 1533033820967474.

Xia, Z., Ou-Yang, W., Hu, T., & Du, K. (2019). Prognostic significance of CDC25C in lung adenocarcinoma: an analysis of TCGA data. Cancer genetics, 233, 67-74.

Li, H., Liang, D., Hu, N., Dai, X., He, J., Zhuang, H., & Zhao, W. (2019). Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade. Journal of periodontal & implant science, 49(3), 138-147.

Jayasooriya, R. G. P. T., Dilshara, M. G., Molagoda, I. M. N., Park, C., Park, S. R., Lee, S., ... & Kim, G. Y. (2018). Camptothecin induces G2/M phase arrest through the ATM-Chk2-Cdc25C axis as a result of autophagy-induced cytoprotection: Implications of reactive oxygen species. Oncotarget, 9(31), 21744.

Lal, N., Nemaysh, V., & Luthra, P. M. (2018). Proteasome mediated degradation of CDC25C and Cyclin B1 in Demethoxycurcumin treated human glioma U87 MG cells to trigger G2/M cell cycle arrest. Toxicology and applied pharmacology, 356, 76-89.

Ojiegbe, S., Joseph, C., Provenzano, E., Caldas, C., Nolan, C., Green, A. R., ... & Mukherjee, A. (2017). Cell division cycle 25C (CDC25C) expression confers poor prognosis in invasive breast cancer.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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