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Mouse Anti-CDK6 Recombinant Antibody (75B9) (CBMAB-C8514-LY)

This product is antibody recognizes CDK6. The antibody 75B9 immunoassay techniques such as: ELISA, FC, ICC, IF, WB.
See all CDK6 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
75B9
Antibody Isotype
IgG2b
Application
ELISA, FC, ICC, IF, WB

Basic Information

Immunogen
Recombinant human protein purified from E.coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.01% BSA, 50% glycerol
Preservative
0.03% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cyclin Dependent Kinase 6
Entrez Gene ID
1021
UniProt ID
Q00534
Alternative Names
Cyclin Dependent Kinase 6; Serine/Threonine-Protein Kinase PLSTIRE; Cell Division Protein Kinase 6; EC 2.7.11.22; Cyclin-Dependent Kinase 6; EC 2.7.11; PLSTIRE; MCPH12; CDKN6;
Function
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).
Biological Process
Astrocyte development Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cell dedifferentiation Source: BHF-UCL
Cell division Source: UniProtKB-KW
Dentate gyrus development Source: UniProtKB
G1/S transition of mitotic cell cycle Source: UniProtKB
Generation of neurons Source: UniProtKB
Gliogenesis Source: BHF-UCL
Lateral ventricle development Source: UniProtKB
Negative regulation of cell cycle Source: UniProtKB
Negative regulation of cell differentiation Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cellular senescence Source: UniProtKB
Negative regulation of epithelial cell proliferation Source: BHF-UCL
Negative regulation of G1/S transition of mitotic cell cycle Source: Reactome
Negative regulation of monocyte differentiation Source: UniProtKB
Negative regulation of myeloid cell differentiation Source: UniProtKB
Negative regulation of osteoblast differentiation Source: BHF-UCL
Positive regulation of cell-matrix adhesion Source: BHF-UCL
Positive regulation of fibroblast proliferation Source: BHF-UCL
Protein phosphorylation Source: BHF-UCL
Regulation of cell cycle Source: GO_Central
Regulation of cell motility Source: UniProtKB
Regulation of erythrocyte differentiation Source: BHF-UCL
Regulation of gene expression Source: BHF-UCL
Response to virus Source: UniProtKB
Type B pancreatic cell development Source: UniProtKB
Cellular Location
Centrosome; Nucleus; Cytoplasm; Ruffle. Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663).
Involvement in disease
Microcephaly 12, primary, autosomal recessive (MCPH12): A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small.
PTM
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

Zhou, H., Jia, X., Yang, F., & Shi, P. (2021). miR-148a-3p suppresses the progression of acute myeloid leukemia via targeting cyclin-dependent kinase 6 (CDK6). Bioengineered, 12(1), 4508-4519.

Luo, X., Zhao, Y., Tang, P., Du, X., Li, F., Wang, Q., ... & He, J. (2021). Discovery of new small-molecule cyclin-dependent kinase 6 inhibitors through computational approaches. Molecular Diversity, 25(1), 367-382.

Yousuf, M., Shamsi, A., Queen, A., Shahbaaz, M., Khan, P., Hussain, A., ... & Imtaiyaz Hassan, M. (2021). Targeting cyclin‐dependent kinase 6 by vanillin inhibits proliferation of breast and lung cancer cells: Combined computational and biochemical studies. Journal of Cellular Biochemistry.

Nusantoro, Y. R., & Fadlan, A. (2021). In Silico Studies on Isatinyl-2-aminobenzoylhydrazone Transition Metal Complexes against Cyclin-dependent Kinase 6 (CDK6). Pharmacy Reports, epr1-epr1.

Yousuf, M., Shamsi, A., Khan, P., Shahbaaz, M., AlAjmi, M. F., Hussain, A., ... & Hassan, M. (2020). Ellagic acid controls cell proliferation and induces apoptosis in breast cancer cells via inhibition of cyclin-dependent kinase 6. International journal of molecular sciences, 21(10), 3526.

Duan, L., Yan, Y., Wang, G., Xing, Y. L., Sun, J., & Wang, L. L. (2020). ΜiR-182-5p functions as a tumor suppressor to sensitize human ovarian cancer cells to cisplatin through direct targeting the cyclin dependent kinase 6 (CDK6). J. BUON, 25, 2279-2286.

Li, F., Jiang, Z., Shao, X., & Zou, N. (2020). Downregulation of lncRNA NR2F2 antisense RNA 1 induces G1 arrest of colorectal cancer cells by downregulating cyclin-dependent kinase 6. Digestive diseases and sciences, 65(2), 464-469.

Jin, J., Chen, X., Chen, J., & Geng, X. (2020). Long noncoding RNA MACC1‑AS1 is a potential sponge of microRNA‑34a in cervical squamous cell carcinoma and upregulates cyclin‑dependent kinase 6. Oncology letters, 19(3), 2339-2345.

Zhang, J., Zhang, L., Xu, Y., Jiang, S., & Shao, Y. (2018). Deciphering the binding behavior of flavonoids to the cyclin dependent kinase 6/cyclin D complex. PloS one, 13(5), e0196651.

Wu, H., Tao, J., Li, X., Zhang, T., Zhao, L., Wang, Y., ... & Song, G. (2017). Microrna‐206 prevents the pathogenesis of hepatocellular carcinoma by modulating expression of met proto‐oncogene and cyclin‐dependent kinase 6 in mice. Hepatology, 66(6), 1952-1967.

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For research use only. Not intended for any clinical use.

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