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Rabbit Anti-CEACAM1 Recombinant Antibody (117) (CBMAB-C0216-LY)

This product is antibody recognizes CEACAM 1 . The antibody 117 immunoassay techniques such as: FC, ICC/IF, IF.
See all CEACAM1 antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse
Clone
117
Antibody Isotype
IgG
Application
FC, ICC/IF, IF

Basic Information

Immunogen
Recombinant Mouse CEACAM1 / CD66a protein
Specificity
Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Carcinoembryonic Antigen Related Cell Adhesion Molecule 1
Introduction
This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined. [provided by RefSeq, May 2010]
Entrez Gene ID
UniProt ID
Function
Isoform 1:
Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity).
Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis (PubMed:18424730, PubMed:23696226, PubMed:25363763).
Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils (PubMed:18424730, PubMed:23696226).
Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70 (PubMed:18424730).
Also inhibits T cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T cell through its interaction with HAVCR2 (PubMed:25363763).
Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation (PubMed:23696226).
Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Downregulates neutrophil production by acting as a coinhibitory receptor for CSF3R by downregulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R (By similarity).
Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling (By similarity).
Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways (By similarity).
Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production (By similarity).
Downregulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 pathway (By similarity).
Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex (By similarity).
Inhibits cell migration and cell scattering through interaction with FLNA; interfers with the interaction of FLNA with RALA (PubMed:16291724).
Mediates bile acid transport activity in a phosphorylation dependent manner (By similarity).
Negatively regulates osteoclastogenesis (By similarity).
Isoform 8:
Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity).
Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens (By similarity).
Biological Process
Angiogenesis Source: UniProtKB
Bile acid and bile salt transport Source: UniProtKB
Blood vessel development Source: UniProtKB
Cell adhesion Source: UniProtKB
Cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to insulin stimulus Source: UniProtKB
Common myeloid progenitor cell proliferation Source: UniProtKB
Granulocyte colony-stimulating factor signaling pathway Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Insulin catabolic process Source: UniProtKB
Insulin receptor internalization Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Leukocyte migration Source: Reactome
Negative regulation of cytotoxic T cell degranulation Source: UniProtKB
Negative regulation of fatty acid biosynthetic process Source: UniProtKB
Negative regulation of granulocyte differentiation Source: UniProtKB
Negative regulation of hepatocyte proliferation Source: UniProtKB
Negative regulation of interleukin-1 production Source: UniProtKB
Negative regulation of lipid biosynthetic process Source: UniProtKB
Negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: UniProtKB
Negative regulation of platelet aggregation Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of T cell mediated cytotoxicity Source: UniProtKB
Negative regulation of T cell receptor signaling pathway Source: UniProtKB
Negative regulation of vascular permeability Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of vasculogenesis Source: UniProtKB
Regulation of blood vessel remodeling Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of cell migration Source: UniProtKB
Regulation of endothelial cell differentiation Source: UniProtKB
Regulation of endothelial cell migration Source: UniProtKB
Regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
Regulation of ERK1 and ERK2 cascade Source: UniProtKB
Regulation of homophilic cell adhesion Source: UniProtKB
Regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
Regulation of sprouting angiogenesis Source: UniProtKB
wound healing, spreading of cells Source: UniProtKB
Cellular Location
Isoform 1: Cell membrane; Lateral cell membrane; Apical cell membrane; Basal cell membrane; Cell junction; Adherens junction. Canalicular domain of hepatocyte plasma membranes. Found as a mixture of monomer, dimer and oligomer in the plasma membrane. Occurs predominantly as cis-dimers and/or small cis-oligomers in the cell junction regions. Found as dimer in the solution. Predominantly localized to the lateral cell membranes.
Isoform 2&3&4: Secreted
Isoform 5&6&7: Cell membrane
Isoform 8: Cell membrane; Lateral cell membrane; Apical cell membrane; Basal cell membrane; Secretory vesicle membrane; Cell junction; Adherens junction. Predominantly localized to the lateral cell membranes. Found as a mixture of monomer, dimer and oligomer in the plasma membrane. Occurs predominantly as cis-dimers and/or small cis-oligomers in the cell junction regions (By similarity). Co-localizes with ANXA2 in secretory vesicles and with S100A10/p11 at the plasma membrane (PubMed:14522961).
Microvillus membrane; Apical cell membrane. Localized to the apical glycocalyx surface (PubMed:10436421). Colocalizes with CEACAM20 at the apical brush border of intestinal cells.
Topology
Extracellular: 35-428
Helical: 429-452
Cytoplasmic: 453-526
PTM
Isoform 1:
Phosphorylated on serine and tyrosine (By similarity). Isoform 1 is phosphorylated on tyrosine by Src family kinases like SRC and LCK and by receptor like CSF3R, EGFR and INSR upon stimulation (PubMed:15467833, PubMed:18424730, PubMed:7478590). Phosphorylated at Ser-508; mediates activity. Phosphorylated at Tyr-493; regulates activity (By similarity). Phosphorylated at Tyr-493 by EGFR and INSR upon stimulation; this phosphorylation is Ser-508-phosphorylation-dependent; mediates cellular internalization; increases interaction with downstream proteins like SHC1 and FASN (By similarity). Phosphorylated at Tyr-493 and Tyr-520 by LCK; mediates PTPN6 association and is regulated by homophilic ligation of CEACAM1 in the absence of T cell activation (PubMed:18424730). Phosphorylated at Tyr-520; mediates interaction with PTPN11 (By similarity).
Isoform 8:
Phosphorylated on serine and threonine.

Hayashi, S., Osada, Y., Miura, K., & Simizu, S. (2020). Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochemistry and biophysics reports, 21, 100734.

Weng, C. Y., Hu, X. Y., & Wang, Y. J. (2020). Integrated analysis of gene expression, alteration and clinical significance of carcinoembryonic antigen-related cell adhesion molecule 1 in cancer. 3 Biotech, 10(3), 1-19.

Kleefeldt, F., Bömmel, H., Broede, B., Thomsen, M., Pfeiffer, V., Wörsdörfer, P., ... & Ergün, S. (2019). Aging‐related carcinoembryonic antigen‐related cell adhesion molecule 1 signaling promotes vascular dysfunction. Aging cell, 18(6), e13025.

Wang, N., Wang, Q., Chi, J., Xiang, F., Lin, M., Wang, W., ... & Feng, Y. (2019). Carcinoembryonic antigen cell adhesion molecule 1 inhibits the antitumor effect of neutrophils in tongue squamous cell carcinoma. Cancer science, 110(2), 519-529.

Calinescu, A., Turcu, G., Nedelcu, R. I., Brinzea, A., Hodorogea, A., Antohe, M., ... & Badarau, I. A. (2018). On the dual role of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) in human malignancies. Journal of immunology research, 2018.

Horst, A. K., Wegscheid, C., Schaefers, C., Schiller, B., Neumann, K., Lunemann, S., ... & Tiegs, G. (2018). Carcinoembryonic antigen‐related cell adhesion molecule 1 controls IL‐2‐dependent regulatory T‐cell induction in immune‐mediated hepatitis in mice. Hepatology, 68(1), 200-214.

Ghadieh, H. E., Muturi, H. T., Russo, L., Marino, C. C., Ghanem, S. S., Khuder, S. S., ... & Najjar, S. M. (2018). Exenatide induces carcinoembryonic antigen‐related cell adhesion molecule 1 expression to prevent hepatic steatosis. Hepatology communications, 2(1), 35-47.

Li, J., Liu, X., Duan, Y., Wang, H., Su, W., Wang, Y., ... & Fan, Y. (2018). Abnormal expression of circulating and tumor-infiltrating carcinoembryonic antigen-related cell adhesion molecule 1 in patients with glioma. Oncology letters, 15(3), 3496-3503.

Xi, R., Pun, I. H. Y., Menezes, S. V., Fouani, L., Kalinowski, D. S., Huang, M. L., ... & Kovacevic, Z. (2017). Novel Thiosemicarbazones Inhibit Lysine-Rich Carcinoembryonic Antigen–Related Cell Adhesion Molecule 1 (CEACAM1) Coisolated (LYRIC) and the LYRIC-Induced Epithelial-Mesenchymal Transition via Upregulation of N-Myc Downstream-Regulated Gene 1 (NDRG1). Molecular pharmacology, 91(5), 499-517.

Heinrich, G., Muturi, H. T., Rezaei, K., Al-Share, Q. Y., DeAngelis, A. M., Bowman, T. A., ... & Najjar, S. M. (2017). Reduced hepatic carcinoembryonic antigen-related cell adhesion molecule 1 level in obesity. Frontiers in endocrinology, 8, 54.

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For research use only. Not intended for any clinical use.

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