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Mouse Anti-CH25H (AA 142-247) Recombinant Antibody (CBFYC-1794) (CBMAB-C1856-FY)

This product is mouse antibody that recognizes CH25H. The antibody CBFYC-1794 can be used for immunoassay techniques such as: ELISA, WB.
See all CH25H antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYC-1794
Antibody Isotype
IgG2b, k
Application
ELISA, WB

Basic Information

Immunogen
CH25H partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: WHLLHHKVPW LYRTFHKVHH QNSSSFALAT QYMSVWELFS LGFFDMMNVT LLGCHPLTTL TFHVVNIWLS VEDHSGYNFP WSTHRLVPFG WYGGVVHHDL HHSHFN
Specificity
Human
Antibody Isotype
IgG2b, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 142-247

Target

Full Name
cholesterol 25-hydroxylase
Introduction
This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Entrez Gene ID
UniProt ID
Alternative Names
Cholesterol 25-Hydroxylase; Cholesterol 25-Monooxygenase; EC 1.14.99.38; H25OH; C25H
Function
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097).
Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
Biological Process
B cell chemotaxis Source: UniProtKB
Bile acid biosynthetic process Source: Reactome
Cholesterol metabolic process Source: GO_Central
Lipid metabolic process Source: ProtInc
Sterol biosynthetic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane
PTM
N-glycosylated.

Madenspacher, J. H., Morrell, E. D., Gowdy, K. M., McDonald, J. G., Thompson, B. M., Muse, G., ... & Fessler, M. B. (2020). Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation. JCI insight, 5(11).

Russo, L., Muir, L., Geletka, L., Delproposto, J., Baker, N., Flesher, C., ... & Lumeng, C. N. (2020). Cholesterol 25-hydroxylase (CH25H) as a promoter of adipose tissue inflammation in obesity and diabetes. Molecular metabolism, 39, 100983.

Sheng, N., Ma, Z., Zhou, Y., Xu, J., Gao, Y., & Fu, X. Y. (2020). Cholesterol 25-hydroxylase protects against experimental colitis in mice by modulating epithelial gut barrier function. Scientific reports, 10(1), 1-12.

Yuan, Y., Wang, Z., Tian, B., Zhou, M., Fu, Z. F., & Zhao, L. (2019). Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry. Archives of virology, 164(12), 2963-2974.

Zhang, Y., Song, Z., Wang, M., Lan, M., Zhang, K., Jiang, P., ... & Wang, X. (2019). Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol. Veterinary microbiology, 231, 129-138.

Liu, Y., Wei, Z., Ma, X., Yang, X., Chen, Y., Sun, L., ... & Duan, Y. (2018). 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism [S]. Journal of lipid research, 59(3), 439-451.

Wang, J., Zeng, L., Zhang, L., Guo, Z. Z., Lu, S. F., Ming, S. L., ... & Chu, B. B. (2017). Cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication. Journal of General Virology, 98(6), 1467-1476.

Ke, W., Fang, L., Jing, H., Tao, R., Wang, T., Li, Y., ... & Xiao, S. (2017). Cholesterol 25-hydroxylase inhibits porcine reproductive and respiratory syndrome virus replication through enzyme activity-dependent and-independent mechanisms. Journal of virology, 91(19), e00827-17.

Li, Z., Martin, M., Zhang, J., Huang, H. Y., Bai, L., Zhang, J., ... & Shyy, J. Y. J. (2017). Krüppel-like factor 4 regulation of cholesterol-25-hydroxylase and liver X receptor mitigates atherosclerosis susceptibility. Circulation, 136(14), 1315-1330.

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For research use only. Not intended for any clinical use.

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