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Mouse Anti-CLIC1 Recombinant Antibody (CBWJN-1074) (CBMAB-N0684-WJ)

This product is a Mouse antibody that recognizes CLIC1. The antibody CBWJN-1074 can be used for immunoassay techniques such as: ELISA, WB.
See all CLIC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJN-1074
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
pH 7.0
Preservative
0.02% Proclin 300
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CLIC1
Introduction
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]
Entrez Gene ID
1192
UniProt ID
O00299
Alternative Names
Chloride Intracellular Channel 1; Regulatory Nuclear Chloride Ion Channel Protein; Nuclear Chloride Ion Channel 27; Chloride Channel ABP; NCC27; HRNCC; G6;
Function
Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
Biological Process
Chloride transport Source: MGI
Glutathione metabolic process Source: InterPro
Platelet aggregation Source: UniProtKB
Positive regulation of osteoblast differentiation Source: Ensembl
Regulation of cell cycle Source: InterPro
Regulation of ion transmembrane transport Source: UniProtKB-KW
Regulation of mitochondrial membrane potential Source: Ensembl
Signal transduction Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm; Nucleus; Nucleus membrane. Mostly in the nucleus including in the nuclear membrane (PubMed:9139710, PubMed:12681486). Small amount in the cytoplasm and the plasma membrane (PubMed:9139710). Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain (PubMed:11940526, PubMed:11551966, PubMed:14613939).
Topology
Helical: 26-46
PTM
Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59.

Cianci, F., & Verduci, I. (2021). Transmembrane Chloride Intracellular Channel 1 (tmCLIC1) as a Potential Biomarker for Personalized Medicine. Journal of Personalized Medicine, 11(7), 635.

Francisco, M. A., Wanggou, S., Fan, J. J., Dong, W., Chen, X., Momin, A., ... & Huang, X. (2020). Chloride intracellular channel 1 cooperates with potassium channel EAG2 to promote medulloblastoma growth. Journal of Experimental Medicine, 217(5).

Zhao, K., Wang, Z., Li, X., Liu, J. L., Tian, L., & Chen, J. Q. (2019). Exosome-mediated transfer of CLIC1 contributes to the vincristine-resistance in gastric cancer. Molecular and cellular biochemistry, 462(1), 97-105.

Yue, X., Cui, Y., You, Q., Lu, Y., & Zhang, J. (2019). MicroRNA‑124 negatively regulates chloride intracellular channel 1 to suppress the migration and invasion of liver cancer cells. Oncology reports, 42(4), 1380-1390.

Lee, J. R., Lee, J. Y., Kim, H. J., Hahn, M. J., Kang, J. S., & Cho, H. (2019). The inhibition of chloride intracellular channel 1 enhances Ca 2+ and reactive oxygen species signaling in A549 human lung cancer cells. Experimental & molecular medicine, 51(7), 1-11.

Barbieri, F., Würth, R., Pattarozzi, A., Verduci, I., Mazzola, C., Cattaneo, M. G., ... & Florio, T. (2018). Inhibition of chloride intracellular channel 1 (CLIC1) as biguanide class-effect to impair human glioblastoma stem cell viability. Frontiers in pharmacology, 9, 899.

Kobayashi, T., Shiozaki, A., Nako, Y., Ichikawa, D., Kosuga, T., Shoda, K., ... & Otsuji, E. (2018). Chloride intracellular channel 1 as a switch among tumor behaviors in human esophageal squamous cell carcinoma. Oncotarget, 9(33), 23237.

Liu, B., Billington, C. K., Henry, A. P., Bhaker, S. K., Kheirallah, A. K., Swan, C., & Hall, I. P. (2018). Chloride intracellular channel 1 (CLIC1) contributes to modulation of cyclic AMP‐activated whole‐cell chloride currents in human bronchial epithelial cells. Physiological reports, 6(2), e13508.

Domingo-Fernández, R., Coll, R. C., Kearney, J., Breit, S., & O'Neill, L. A. (2017). The intracellular chloride channel proteins CLIC1 and CLIC4 induce IL-1β transcription and activate the NLRP3 inflammasome. Journal of Biological Chemistry, 292(29), 12077-12087.

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For research use only. Not intended for any clinical use.

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