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Mouse Anti-CLK1 Recombinant Antibody (CBWJC-2808) (CBMAB-C3925WJ)

This product is a Mouse antibody that recognizes CLK1. This antibody CBWJC-2808 can be used for immunoassay techniques such as: WB, IHC.
See all CLK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJC-2808
Antibody Isotype
IgM
Application
WB, IHC

Basic Information

Immunogen
Clk1-fusion protein
Specificity
Human
Antibody Isotype
IgM
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
CDC Like Kinase 1
Introduction
This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
Entrez Gene ID
1195
UniProt ID
P49759
Alternative Names
CDC Like Kinase 1; CDC-Like Kinase 1; EC 2.7.12.1; CLK; Dual Specificity Protein Kinase CLK1; Protein Tyrosine Kinase STY; CDC28/CDC2-Like Kinase; CLK/STY; STY;
Function
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
Biological Process
Peptidyl-serine phosphorylation Source: Ensembl
Peptidyl-threonine phosphorylation Source: Ensembl
Protein autophosphorylation Source: Ensembl
Regulation of RNA splicing Source: UniProtKB
Cellular Location
Nucleus
PTM
Autophosphorylates on all three types of residues.

Dekel, N., Eisenberg-Domovich, Y., Karlas, A., Meyer, T. F., Bracher, F., Lebendiker, M., ... & Livnah, O. (2020). Expression, purification and crystallization of CLK1 kinase–A potential target for antiviral therapy. Protein Expression and Purification, 176, 105742.

Babu, N., Pinto, S. M., Biswas, M., Subbannayya, T., Rajappa, M., Mohan, S. V., ... & Chatterjee, A. (2020). Phosphoproteomic analysis identifies CLK1 as a novel therapeutic target in gastric cancer. Gastric Cancer, 23(5), 796-810.

Artarini, A., Meyer, M., Shin, Y. J., Huber, K., Hilz, N., Bracher, F., ... & Karlas, A. (2019). Regulation of influenza A virus mRNA splicing by CLK1. Antiviral research, 168, 187-196.

Sun, L., Cheong, J. E., Zaffagni, M., Zhou, K., & Zetter, B. (2018). Discovery of novel water-soluble derivatives of mebendazole as selective CLK1/4 kinase inhibitors and their anticancer cancer activity.

Uzor, S., Zorzou, P., Bowler, E., Porazinski, S., Wilson, I., & Ladomery, M. (2018). Autoregulation of the human splice factor kinase CLK1 through exon skipping and intron retention. Gene, 670, 46-54.

Sako, Y., Ninomiya, K., Okuno, Y., Toyomoto, M., Nishida, A., Koike, Y., ... & Hagiwara, M. (2017). Development of an orally available inhibitor of CLK1 for skipping a mutated dystrophin exon in Duchenne muscular dystrophy. Scientific reports, 7(1), 1-9.

Sun, Q. Z., Lin, G. F., Li, L. L., Jin, X. T., Huang, L. Y., Zhang, G., ... & Yang, S. Y. (2017). Discovery of potent and selective inhibitors of cdc2-like kinase 1 (clk1) as a new class of autophagy inducers. Journal of medicinal chemistry, 60(14), 6337-6352.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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