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Rat Anti-CRB3 Recombinant Antibody (CBCNC-342) (CBMAB-C2802-CN)

This product is a Rat antibody that recognizes CRB3. The antibody CBCNC-342 can be used for immunoassay techniques such as: ELISA.
See all CRB3 antibodies

Summary

Host Animal
Rat
Specificity
Human
Clone
CBCNC-342
Antibody Isotype
IgG2c
Application
ELISA

Basic Information

Immunogen
Full length
Specificity
Human
Antibody Isotype
IgG2c
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
Crumbs 3, Cell Polarity Complex Component
Introduction
CRB3 (Crumbs 3, Cell Polarity Complex Component) is a Protein Coding gene. Diseases associated with CRB3 include Leber Congenital Amaurosis 8 and Leber Congenital Amaurosis. Among its related pathways are Notch Signaling Pathway (sino) and PI3K-Akt signaling pathway. Gene Ontology (GO) annotations related to this gene include protein domain specific binding and SH3 domain binding.
Entrez Gene ID
92359
UniProt ID
Q9BUF7
Alternative Names
Crumbs 3, Cell Polarity Complex Component; Crumbs Homolog 3 (Drosophila); Crumbs Family Member 3;
Function
Involved in the establishment of cell polarity in mammalian epithelial cells (PubMed:12771187, PubMed:14718572).

Regulates the morphogenesis of tight junctions (PubMed:12771187, PubMed:14718572).

Involved in promoting phosphorylation and cytoplasmic retention of transcriptional coactivators YAP1 and WWTR1/TAZ which leads to suppression of TGFB1-dependent transcription of target genes such as CCN2/CTGF, SERPINE1/PAI1, SNAI1/SNAIL1 and SMAD7 (By similarity).
Biological Process
Bicellular tight junction assembly Source: Reactome
Positive regulation of cell junction assembly Source: UniProtKB
Protein localization to plasma membrane Source: BHF-UCL
Cellular Location
Apical cell membrane; Tight junction. Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells.
Topology
Extracellular: 27-59
Helical: 60-80
Cytoplasmic: 81-120

Pan, J., Liu, F., Xiao, X., Xu, R., Dai, L., Zhu, M., ... & Ji, G. (2022). METTL3 promotes colorectal carcinoma progression by regulating the m6A–CRB3–Hippo axis. Journal of Experimental & Clinical Cancer Research, 41(1), 1-17.

Khalilian, S., Hojati, Z., Dehghanian, F., Shaygannejad, V., Imani, S. Z. H., Kheirollahi, M., ... & Mirmosayyeb, O. (2021). Gene expression profiles of YAP1, TAZ, CRB3, and VDR in familial and sporadic multiple sclerosis among an Iranian population. Scientific reports, 11(1), 1-10.

Li, P., Zhou, C., Yan, Y., Li, J., Liu, J., Zhang, Y., & Liu, P. (2019). Crumbs protein homolog 3 (CRB3) expression is associated with oestrogen and progesterone receptor positivity in breast cancer. Clinical and Experimental Pharmacology and Physiology, 46(9), 837-844.

Madigan, V. J., Tyson, T. O., Yuziuk, J. A., Pillai, M., Moller-Tank, S., & Asokan, A. (2019). A CRISPR screen identifies the cell polarity determinant crumbs 3 as an adeno-associated virus restriction factor in hepatocytes. Journal of virology, 93(21), e00943-19.

Li, P., Liu, J., Li, J., & Liu, P. (2019). DNA methylation of CRB3 is a prognostic biomarker in clear cell renal cell carcinoma. Molecular biology reports, 46(4), 4377-4383.

Mao, X., Li, P., Wang, Y., Liang, Z., Liu, J., Li, J., ... & Liu, P. (2018). CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells. Cell death & disease, 8(1), e2546-e2546.

Li, P., Feng, C., Chen, H., Jiang, Y., Cao, F., Liu, J., & Liu, P. (2018). Elevated CRB 3 expression suppresses breast cancer stemness by inhibiting β‐catenin signalling to restore tamoxifen sensitivity. Journal of cellular and molecular medicine, 22(7), 3423-3433.

Walker, S. J., Selfors, L. M., Margolis, B. L., & Brugge, J. S. (2018). CRB3 and the FERM protein EPB41L4B regulate proliferation of mammary epithelial cells through the release of amphiregulin. Plos one, 13(11), e0207470.

Li, P., Wang, Y., Mao, X., Jiang, Y., Liu, J., Li, J., ... & Liu, P. (2017). CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin. Oncogenesis, 6(4), e322-e322.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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