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Mouse Anti-CREBBP Recombinant Antibody (NM11) (CBMAB-K0004-LY)

This product is antibody recognizes CREBBP. The antibody NM11 immunoassay techniques such as: IHC-P, ICC/IF.
See all CREBBP antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Monkey
Clone
NM11
Antibody Isotype
IgG2b
Application
IHC-P, ICC/IF

Basic Information

Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CREB binding protein
Entrez Gene ID
Human1387
Mouse12914
Rat54244
Monkey704331
UniProt ID
HumanQ92793
MouseP45481
RatQ6JHU9
MonkeyA0A5F7ZKK8
Alternative Names
CREB Binding Protein; EC 2.3.1.48; CBP; Rubinstein-Taybi Syndrome; CREB-Binding Protein; KAT3A; RSTS1; RSTS;
Function
Acetylates histones, giving a specific tag for transcriptional activation (PubMed:24616510).

Also acetylates non-histone proteins, like DDX21, FBL, IRF2, MAFG, NCOA3, POLR1E/PAF53 and FOXO1 (PubMed:10490106, PubMed:11154691, PubMed:12738767, PubMed:12929931, PubMed:9707565, PubMed:24207024, PubMed:28790157, PubMed:30540930).

Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers (PubMed:14645221).

Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902).

Acetylates POLR1E/PAF53, leading to decreased association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:24207024).

Acetylates DDX21, thereby inhibiting DDX21 helicase activity (PubMed:28790157).

Acetylates FBL, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me) (PubMed:30540930).

Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (PubMed:25514493).
Biological Process
Beta-catenin-TCF complex assembly Source: Reactome
Cellular response to UV Source: UniProtKB
Embryonic digit morphogenesis Source: BHF-UCL
Histone acetylation Source: UniProtKB
Histone glutamine methylation Source: UniProtKB
Homeostatic process Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Notch signaling pathway Source: Reactome
N-terminal peptidyl-lysine acetylation Source: UniProtKB
Positive regulation of Notch signaling pathway Source: Reactome
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: HGNC-UCL
Positive regulation of transcription of Notch receptor target Source: Reactome
Positive regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
Positive regulation of type I interferon production Source: Reactome
Protein acetylation Source: UniProtKB
Protein-containing complex assembly Source: ProtInc
Protein destabilization Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cellular response to heat Source: Reactome
Regulation of lipid metabolic process Source: Reactome
Regulation of myeloid cell differentiation Source: Reactome
Regulation of smoothened signaling pathway Source: BHF-UCL
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Response to hypoxia Source: UniProtKB
Rhythmic process Source: UniProtKB-KW
Signal transduction Source: ProtInc
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Transcription initiation from RNA polymerase II promoter Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
Involvement in disease
Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with KAT6A; translocation t(11;16)(q23;p13.3) with KMT2A/MLL1; translocation t(10;16)(q22;p13) with KAT6B. KAT6A-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Rubinstein-Taybi syndrome 1 (RSTS1):
A disorder characterized by craniofacial abnormalities, postnatal growth deficiency, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
Menke-Hennekam syndrome 1 (MKHK1):
A form of Menke-Hennekam syndrome, a congenital autosomal dominant disease characterized by developmental delay, growth retardation, and craniofacial dysmorphism. Patients have intellectual disability of variable severity, speech delay, autistic behavior, short stature and microcephaly. Main facial characteristics include short palpebral fissures, telecanthi, depressed nasal ridge, short nose, anteverted nares, short columella and long philtrum.
PTM
Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response (By similarity).
Phosphorylated by CHUK/IKKA at Ser-1382 and Ser-1386; these phosphorylations promote cell growth by switching the binding preference of CREBBP from TP53 to NF-kappa-B.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
Autoacetylation is required for binding to protein substrates, such as acetylated histones and acetylated TP53/p53.

Fraguas, S., Cárcel, S., Vivancos, C., Molina, M. D., Ginés, J., Mazariegos, J., ... & Cebrià, F. (2021). CREB-binding protein (CBP) gene family regulates planarian survival and stem cell differentiation. Developmental Biology, 476, 53-67.

Wu, S. L., Zhao, J., Sun, H. B., Li, H. Y., Yin, Y. Y., & Zhang, L. L. (2021). Insights into interaction mechanism of inhibitors E3T, E3H and E3B with CREB binding protein by using molecular dynamics simulations and MM-GBSA calculations. SAR and QSAR in Environmental Research, 32(3), 221-246.

Akinsiku, O. E., Soremekun, O. S., & Soliman, M. E. (2021). Update and Potential Opportunities in CBP [Cyclic Adenosine Monophosphate (cAMP) Response Element-Binding Protein (CREB)-Binding Protein] Research Using Computational Techniques. The protein journal, 40(1), 19-27.

Sadeghi, H., Esmkhani, S., Pirjani, R., Amin-Beidokhti, M., Gholami, M., Azizi Tabesh, G., ... & Mirfakhraie, R. (2021). CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene. Molecular Biology Reports, 48(3), 2117-2122.

Ozbek, P. (2020). Revisiting allostery in CREB-binding protein (CBP) using residue-based interaction energy. Journal of Computer-Aided Molecular Design, 34(9), 965-974.

Garcia, A. M., Giorgiutti, C., El Khoury, Y., Bauer, V., Spiegelhalter, C., Leize‐Wagner, E., ... & Torbeev, V. (2020). Aggregation and Amyloidogenicity of the Nuclear Coactivator Binding Domain of CREB‐Binding Protein. Chemistry–A European Journal, 26(44), 9889-9899.

Osawa, Y., Kojika, E., Nishikawa, K., Kimura, M., Osakaya, S., Miyauchi, H., ... & Kimura, K. (2019). Programmed cell death ligand 1 (PD-L1) blockade attenuates metastatic colon cancer growth in cAMP-response element-binding protein (CREB)-binding protein (CBP)/β-catenin inhibitor-treated livers. Oncotarget, 10(32), 3013.

Jiang, M., Yan, Y., Yang, K., Liu, Z., Qi, J., Zhou, H., ... & Deng, L. (2019). Small molecule nAS‐E targeting cAMP response element binding protein (CREB) and CREB‐binding protein interaction inhibits breast cancer bone metastasis. Journal of cellular and molecular medicine, 23(2), 1224-1234.

Wei, J., Dong, S., Bowser, R. K., Khoo, A., Zhang, L., Jacko, A. M., ... & Zhao, J. (2017). Regulation of the ubiquitylation and deubiquitylation of CREB-binding protein modulates histone acetylation and lung inflammation. Science signaling, 10(483), eaak9660.

Roy, A., George, S., & Palli, S. R. (2017). Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. BMC genomics, 18(1), 1-14.

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For research use only. Not intended for any clinical use.

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