Mouse Anti-CRYBB1 Recombinant Antibody (2B2) (CBMAB-C4684-LY)

This product is antibody recognizes CRYBB1. The antibody 2B2 immunoassay techniques such as: ELISA, WB.
See all CRYBB1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2B2

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Immunogen

CRYBB1 (AAH36790.1, 1 a.a. ~ 252 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.2-1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Crystallin Beta B1

Introduction

CRYBB1 (Crystallin Beta B1) is a Protein Coding gene. Diseases associated with CRYBB1 include Cataract 17, Multiple Types and Cataract Microcornea Syndrome. Gene Ontology (GO) annotations related to this gene include structural constituent of eye lens. An important paralog of this gene is CRYBB3.

Entrez Gene ID

1414

UniProt ID

P53674

Alternative Names

Crystallin Beta B1; Beta-B1 Crystallin; Eye Lens Structural Protein; Crystallin, Beta B1; Beta-Crystallin B1; CTRCT17; CATCN3;

Function

Crystallins are the dominant structural components of the vertebrate eye lens.

Biological Process

Lens development in camera-type eye Source: GO_Central
Visual perception Source: GO_Central

Involvement in disease

Cataract 17, multiple types (CTRCT17):
An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. CTRCT17 includes nuclear and pulverulent cataracts, among others. Nuclear cataracts affect the central nucleus of the eye, are often not highly visually significant. The density of the opacities varies greatly from fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral. Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear opacity, which are referred to as cortical riders. Pulverulent cataracts are characterized by a dust-like, 'pulverised' appearance of the opacities which can be found in any part of the lens.
CRYBB1 mutations may be a cause of congenital cataract and microcornea syndrome, a disease characterized by the association of congenital cataract and microcornea without any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most often bilateral posterior polar with opacification in the lens periphery. The cataract progresses to form a total cataract after visual maturity has been achieved, requiring cataract extraction in the first to third decade of life (PubMed:16110300 and PubMed:21972112).

PTM

Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization.

References

Ngqaneka, T., Khoza, S., Magwebu, Z. E., & Chauke, C. G. (2020). Mutational analysis of BFSP1, CRYBB1, GALK1, and GJA8 in captive‐bred vervet monkeys (Chlorocebus aethiops). Journal of Medical Primatology, 49(2), 79-85.

Jin, A., Zhang, Y., Xiao, D., Xiang, M., Jin, K., & Zeng, M. (2020). A novel mutation p. S93R in CRYBB1 associated with dominant congenital cataract and microphthalmia. Current Eye Research, 45(4), 483-489.

Graw, J., Amarie, O., Kumar, D., Scheideler, A., Hrabé de Angelis, M., Przemeck, G., & Sabrautzki, S. (2017). Mutation in the Crybb1 gene encoding beta‐B1‐crystallin leads to recessive cataracts in the mouse. Acta Ophthalmologica, 95.

Rao, Y., Dong, S., Li, Z., Yang, G., Peng, C., Yan, M., & Zheng, F. (2017). A novel truncation mutation in CRYBB1 associated with autosomal dominant congenital cataract with nystagmus. Molecular Vision, 23, 624.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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