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Mouse Anti-CUL4B Recombinant Antibody (4E10B10) (CBMAB-C6835-LY)

This product is antibody recognizes CUL4B. The antibody 4E10B10 immunoassay techniques such as: siRNA, ELISA, IHC-F, WB.
See all CUL4B antibodies

Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
4E10B10
Antibody Isotype
IgG1
Application
siRNA, ELISA, IHC-F, WB

Basic Information

Specificity
Human, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cullin 4B
Introduction
CUL4B (Cullin 4B) is a Protein Coding gene. Diseases associated with CUL4B include Mental Retardation, X-Linked, Syndromic, Cabezas Type and Seizure Disorder. Among its related pathways are Mesodermal Commitment Pathway and Transcription-Coupled Nucleotide Excision Repair (TC-NER). Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding and damaged DNA binding.
An important paralog of this gene is CUL4A.
Entrez Gene ID
Human8450
Rat302502
UniProt ID
HumanQ13620
RatD3ZK73
Function
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. With CUL4A, contributes to ribosome biogenesis (PubMed:26711351).
Biological Process
Cellular response to DNA damage stimulus Source: GO_Central
DNA damage response, detection of DNA damage Source: Reactome
G1/S transition of mitotic cell cycle Source: UniProtKB
Global genome nucleotide-excision repair Source: Reactome
Histone H2A monoubiquitination Source: UniProtKB
Nucleotide-excision repair, DNA damage recognition Source: Reactome
Nucleotide-excision repair, DNA duplex unwinding Source: Reactome
Nucleotide-excision repair, DNA incision Source: Reactome
Nucleotide-excision repair, DNA incision, 3'-to lesion Source: Reactome
Nucleotide-excision repair, DNA incision, 5'-to lesion Source: Reactome
Nucleotide-excision repair, preincision complex assembly Source: Reactome
Nucleotide-excision repair, preincision complex stabilization Source: Reactome
Post-translational protein modification Source: Reactome
Proteasomal protein catabolic process Source: MGI
Protein ubiquitination Source: UniProtKB
Ribosome biogenesis Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: GO_Central
Transcription-coupled nucleotide-excision repair Source: Reactome
UV-damage excision repair Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Mental retardation, X-linked, syndromic, 15 (MRXS15):
A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span.
PTM
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.

Fan, Y., Huo, X., Guo, B., Zhang, X., Yang, Y., Lian, J., ... & Hu, H. (2022). Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis. Cell Death & Differentiation, 1-16.

Gu, Z., You, Z., Yang, Y., Ding, R., Wang, M., Pu, J., & Chen, J. (2021). Inhibition of MicroRNA miR-101-3p on prostate cancer progression by regulating Cullin 4B (CUL4B) and PI3K/AKT/mTOR signaling pathways. Bioengineered, 12(1), 4719-4735.

Yang, H. F., Wang, Z. L., Mao, T. T., & Liu, J. C. (2021). Cullin 4B regulates cell survival and apoptosis in clear cell renal cell carcinoma as a target of microRNA‐217. The Kaohsiung Journal of Medical Sciences, 37(2), 121-127.

Zhong, M., Zhou, L., Zou, J., He, Y., Fang, Z., & Xiang, X. (2021). Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer. Pathology-Research and Practice, 224, 153520.

Li, Y., Hu, H., Wang, Y., Fan, Y., Yang, Y., Guo, B., ... & Gong, Y. (2020). CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis, 9(2), 1-14.

Wu, P., Hu, H., Li, J., & Gong, W. (2020). Upregulation of cullin 4B promotes gastric cancer and predicts poor prognosis. OncoTargets and therapy, 13, 1235.

Zhang, H., Wang, A., Tan, Y., Wang, S., Ma, Q., Chen, X., & He, Z. (2019). NCBP1 promotes the development of lung adenocarcinoma through up‐regulation of CUL4B. Journal of Cellular and Molecular Medicine, 23(10), 6965-6977.

Chen, B., Feng, Y., Zhang, M., Cheng, G., Chen, B., & Wang, H. (2019). Small molecule TSC01682 inhibits osteosarcoma cell growth by specifically disrupting the CUL4B-DDB1 interaction and decreasing the ubiquitination of CRL4B E3 ligase substrates. American journal of cancer research, 9(9), 1857.

Zhao, M., Qi, M., Li, X., Hu, J., Zhang, J., Jiao, M., ... & Han, B. (2019). CUL4B/miR‐33b/C‐MYC axis promotes prostate cancer progression. The Prostate, 79(5), 480-488.

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For research use only. Not intended for any clinical use.

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