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Rat Anti-CX3CL1 Recombinant Antibody (CBFYC-2480) (CBMAB-C2553-FY)

This product is rat antibody that recognizes CX3CL1. The antibody CBFYC-2480 can be used for immunoassay techniques such as: WB, Neut.
See all CX3CL1 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBFYC-2480
Antibody Isotype
IgG2
Application
WB, Neut

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
C-X3-C Motif Chemokine Ligand 1
Introduction
The CX3C chemokine fractalkine (CX3CL1) exists as a membrane-expressed protein promoting cell-cell adhesion and as a soluble molecule inducing chemotaxis.
Entrez Gene ID
20312
UniProt ID
O35188
Alternative Names
CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
Function
Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1 (PubMed:9782118, PubMed:12055230, PubMed:23125415, PubMed:9931005, PubMed:21829356).

The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis (PubMed:9024663, PubMed:9177350, PubMed:9782118, PubMed:12055230).

Regulates leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350).

Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner (PubMed:23125415, PubMed:24789099).

In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins (PubMed:23125415, PubMed:24789099).

In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099).

Processed fractalkine:
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.

Fractalkine:
The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.
Biological Process
Aging Source: ARUK-UCL
Autocrine signaling Source: ARUK-UCL
Cell adhesion Source: ARUK-UCL
Cell-cell adhesion Source: ARUK-UCL
Cell-cell signaling Source: ARUK-UCL
Cell chemotaxis Source: ARUK-UCL
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: ARUK-UCL
Chemotaxis Source: UniProtKB
Cytokine-mediated signaling pathway Source: UniProtKB
Defense response Source: UniProtKB
Eosinophil chemotaxis Source: GO_Central
G protein-coupled receptor signaling pathway Source: ARUK-UCL
Immune response Source: UniProtKB
Inflammatory response Source: GO_Central
Integrin activation Source: UniProtKB
Leukocyte adhesive activation Source: UniProtKB
Leukocyte chemotaxis Source: UniProtKB
Leukocyte migration involved in inflammatory response Source: UniProtKB
Lymphocyte chemotaxis Source: GO_Central
Microglial cell activation Source: ARUK-UCL
Microglial cell proliferation Source: ARUK-UCL
Monocyte chemotaxis Source: GO_Central
Negative regulation of apoptotic process Source: ARUK-UCL
Negative regulation of apoptotic signaling pathway Source: ARUK-UCL
Negative regulation of cell migration Source: BHF-UCL
Negative regulation of cell-substrate adhesion Source: ARUK-UCL
Negative regulation of glutamate receptor signaling pathway Source: ARUK-UCL
Negative regulation of hippocampal neuron apoptotic process Source: ARUK-UCL
Negative regulation of interleukin-1 alpha production Source: ARUK-UCL
Negative regulation of interleukin-1 beta production Source: ARUK-UCL
Negative regulation of interleukin-6 production Source: ARUK-UCL
Negative regulation of microglial cell activation Source: ARUK-UCL
Negative regulation of neuron migration Source: ARUK-UCL
Negative regulation of tumor necrosis factor production Source: ARUK-UCL
Neuron cellular homeostasis Source: ARUK-UCL
Neuron remodeling Source: ARUK-UCL
Neutrophil chemotaxis Source: GO_Central
Positive chemotaxis Source: ARUK-UCL
Positive regulation of actin filament bundle assembly Source: ARUK-UCL
Positive regulation of calcium-independent cell-cell adhesion Source: UniProtKB
Positive regulation of cell-matrix adhesion Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: ARUK-UCL
Positive regulation of I-kappaB phosphorylation Source: ARUK-UCL
Positive regulation of inflammatory response Source: UniProtKB
Positive regulation of MAPK cascade Source: ARUK-UCL
Positive regulation of microglial cell migration Source: ARUK-UCL
Positive regulation of neuroblast proliferation Source: ARUK-UCL
Positive regulation of neuron projection development Source: ARUK-UCL
Positive regulation of NF-kappaB transcription factor activity Source: ARUK-UCL
Positive regulation of protein kinase B signaling Source: ARUK-UCL
Positive regulation of release of sequestered calcium ion into cytosol Source: ARUK-UCL
Positive regulation of smooth muscle cell proliferation Source: ARUK-UCL
Positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
Regulation of lipopolysaccharide-mediated signaling pathway Source: ARUK-UCL
Regulation of neurogenesis Source: ARUK-UCL
Regulation of synaptic plasticity Source: ARUK-UCL
Response to ischemia Source: ARUK-UCL
Synapse pruning Source: ARUK-UCL
Cellular Location
Cell membrane
Processed fractalkine: Secreted
Topology
Extracellular: 25-341
Helical: 342-362
Cytoplasmic: 363-397
PTM
A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans.

Yun, J., Ren, J., Liu, Y., Dai, L., Song, L., Ma, X., ... & Song, Y. (2022). MicroRNA (miR)-590-3p alleviates high-glucose induced renal tubular epithelial cell damage by targeting C-X3-C motif chemokine ligand 1 (CX3CL1) in diabetic nephropathy. Bioengineered, 13(1), 634-644.

Guo, S., Dong, L., Li, J., Chen, Y., Yao, Y., Zeng, R., ... & Rong, S. (2021). C-X3-C motif chemokine ligand 1/receptor 1 regulates the M1 polarization and chemotaxis of macrophages after hypoxia/reoxygenation injury. Chronic diseases and translational medicine, 7(04), 254-265.

Huang, S. J., Chen, C. P., Buchwalder, L., Yu, Y. C., Piao, L., Huang, C. Y., ... & Lockwood, C. J. (2019). Regulation of CX3CL1 expression in human first-trimester Decidual cells: implications for preeclampsia. Reproductive Sciences, 26(9), 1256-1265.

Marchica, V., Toscani, D., Corcione, A., Bolzoni, M., Storti, P., Vescovini, R., ... & Giuliani, N. (2019). Bone marrow CX3CL1/fractalkine is a new player of the pro-angiogenic microenvironment in multiple myeloma patients. Cancers, 11(3), 321.

Liu, J., Li, Y., Zhu, X., Li, Q., Liang, X., Xie, J., ... & Li, C. (2019). Increased CX3CL1 mRNA expression level is a positive prognostic factor in patients with lung adenocarcinoma. Oncology letters, 17(6), 4877-4890.

Liu, W., Liang, Y., Chan, Q., Jiang, L., & Dong, J. (2019). CX3CL1 promotes lung cancer cell migration and invasion via the Src/focal adhesion kinase signaling pathway. Oncology Reports, 41(3), 1911-1917.

Liu, P., Liang, Y., Jiang, L., Wang, H., Wang, S., & Dong, J. (2018). CX3CL1/fractalkine enhances prostate cancer spinal metastasis by activating the Src/FAK pathway. International journal of oncology, 53(4), 1544-1556.

Zhang, J., Liu, Y., Liu, X., Li, S., Cheng, C., Chen, S., & Le, W. (2018). Dynamic changes of CX3CL1/CX3CR1 axis during microglial activation and motor neuron loss in the spinal cord of ALS mouse model. Translational neurodegeneration, 7(1), 1-14.

Miranda, D. O., Anatriello, E., Azevedo, L. R., Santos, J. C., Cordeiro, J. F. C., Peria, F. M., ... & Pereira‑Da‑Silva, G. (2017). Fractalkine (C‑X3‑C motif chemokine ligand 1) as a potential biomarker for depression and anxiety in colorectal cancer patients. Biomedical Reports, 7(2), 188-192.

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For research use only. Not intended for any clinical use.

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