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Human Anti-CXCR3 Recombinant Antibody (CBXC-0758) (CBMAB-C1872-CQ)

This product is a recombinant human antibody that recognizes CXCR3. The antibody CBXC-0758 can be used for immunoassay techniques such as: FC.
See all CXCR3 antibodies

Summary

Host Animal
Human
Specificity
Human
Clone
CBXC-0758
Antibody Isotype
IgG1
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Buffer containing stabilizer
Preservative
0.05% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
C-X-C Motif Chemokine Receptor 3
Introduction
CXCR3 (C-X-C Motif Chemokine Receptor 3) is a Protein Coding gene. Diseases associated with CXCR3 include Cutaneous Lupus Erythematosus and Pulmonary Sarcoidosis. Among its related pathways are Akt Signaling and Peptide ligand-binding receptors. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and chemokine receptor activity. An important paralog of this gene is CXCR5.
Entrez Gene ID
UniProt ID
Alternative Names
C-X-C Motif Chemokine Receptor 3; G Protein-Coupled Receptor 9; Interferon-Inducible Protein 10 Receptor; Chemokine (C-X-C Motif) Receptor 3; IP-10 Receptor; CKR-L2; GPR9; C-X-C Chemokine Receptor Type 3; Chemokine Receptor 3; CD183 Antigen;
Function
Isoform 1:
Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716).

Binds to CCL21. Probably promotes cell chemotaxis response.

Isoform 2:
Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway (PubMed:12782716).

Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis.

Isoform 3:
Mediates the activity of CXCL11.
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Angiogenesis Source: UniProtKB-KW
Apoptotic process Source: UniProtKB-KW
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ProtInc
Cell chemotaxis Source: GO_Central
Cell surface receptor signaling pathway Source: BHF-UCL
Chemokine (C-C motif) ligand 11 production Source: UniProtKB
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: UniProtKB
Immune response Source: GO_Central
Inflammatory response Source: InterPro
Negative regulation of angiogenesis Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of execution phase of apoptosis Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of chemotaxis Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of execution phase of apoptosis Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of cell adhesion Source: UniProtKB
Regulation of leukocyte migration Source: InterPro
T cell chemotaxis Source: Ensembl
Cellular Location
Isoform 1&2: Cell membrane
Topology
Extracellular: 1-53
Helical: 54-80
Cytoplasmic: 81-89
Helical: 90-110
Extracellular: 111-125
Helical: 126-147
Cytoplasmic: 148-169
Helical: 170-189
Extracellular: 190-212
Helical: 213-233
Cytoplasmic: 234-255
Helical: 256-277
Extracellular: 278-298
Helical: 299-321
Cytoplasmic: 322-368
PTM
Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.
N-glycosylated.

Zheng, K., Smith, J. S., Eiger, D. S., Warman, A., Choi, I., Honeycutt, C. C., ... & Rajagopal, S. (2022). Biased agonists of the chemokine receptor CXCR3 differentially signal through Gαi: β-arrestin complexes. Science Signaling, 15(726), eabg5203.

Kundu, N., Ma, X., Brox, R., Fan, X., Kochel, T., Reader, J., ... & Fulton, A. (2019). The chemokine receptor CXCR3 isoform B drives breast cancer stem cells. Breast Cancer: Basic and Clinical Research, 13, 1178223419873628.

Brox, R., Milanos, L., Saleh, N., Baumeister, P., Buschauer, A., Hofmann, D., ... & Tschammer, N. (2018). Molecular mechanisms of biased and probe-dependent signaling at CXC-motif chemokine receptor CXCR3 induced by negative allosteric modulators. Molecular Pharmacology, 93(4), 309-322.

Kim, B., Lee, J. H., Jin, W. J., Kim, H. H., Ha, H., & Lee, Z. H. (2018). JN-2, a CXC motif chemokine receptor 3 antagonist, ameliorates arthritis progression in an animal model. European Journal of Pharmacology, 823, 1-10.

Smith, J. S., Alagesan, P., Desai, N. K., Pack, T. F., Wu, J. H., Inoue, A., ... & Rajagopal, S. (2017). CXC motif chemokine receptor 3 splice variants differentially activate beta-arrestins to regulate downstream signaling pathways. Molecular pharmacology, 92(2), 136-150.

Metzemaekers, M., Vanheule, V., Janssens, R., Struyf, S., & Proost, P. (2018). Overview of the mechanisms that may contribute to the non-redundant activities of interferon-inducible CXC chemokine receptor 3 ligands. Frontiers in immunology, 8, 1970.

Liu, Q. Z., Ma, W. T., Yang, J. B., Zhao, Z. B., Yan, K., Yao, Y., ... & Lian, Z. X. (2018). The CXC chemokine receptor 3 inhibits autoimmune cholangitis via CD8+ T cells but promotes colitis via CD4+ T cells. Frontiers in immunology, 9, 1090.

Weigold, F., Günther, J., Pfeiffenberger, M., Cabral-Marques, O., Siegert, E., Dragun, D., ... & Riemekasten, G. (2018). Antibodies against chemokine receptors CXCR3 and CXCR4 predict progressive deterioration of lung function in patients with systemic sclerosis. Arthritis research & therapy, 20(1), 1-9.

Ma, H. D., Ma, W. T., Liu, Q. Z., Zhao, Z. B., Tsuneyama, K., Gao, J. M., ... & Lian, Z. X. (2017). Chemokine receptor CXCR3 deficiency exacerbates murine autoimmune cholangitis by promoting pathogenic CD8+ T cell activation. Journal of autoimmunity, 78, 19-28.

Priyathilaka, T. T., Oh, M., Bathige, S. D. N. K., De Zoysa, M., & Lee, J. (2017). Two distinct CXC chemokine receptors (CXCR3 and CXCR4) from the big-belly seahorse Hippocampus abdominalis: Molecular perspectives and immune defensive role upon pathogenic stress. Fish & Shellfish Immunology, 65, 59-70.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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