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Mouse Anti-CYP1A1 Recombinant Antibody (CBXC-2976) (CBMAB-C2374-CQ)

This product is a mouse antibody that recognizes CYP1A1. The antibody CBXC-2976 can be used for immunoassay techniques such as: ELISA, WB.
See all CYP1A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-2976
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 1 Subfamily A Member 1
Introduction
CYP1A1 (Cytochrome P450 Family 1 Subfamily A Member 1) is a Protein Coding gene. Diseases associated with CYP1A1 include Aryl Hydrocarbon Hydroxylase Inducibility and Ehrlich Tumor Carcinoma. Among its related pathways are Estrogen Receptor Pathway and Aryl Hydrocarbon Receptor. Gene Ontology (GO) annotations related to this gene include enzyme binding and iron ion binding. An important paralog of this gene is CYP1A2.
Entrez Gene ID
1543
UniProt ID
P04798
Alternative Names
Cytochrome P450 Family 1 Subfamily A Member 1; Cytochrome P450, Subfamily I (Aromatic Compound-Inducible), Polypeptide 1; Cytochrome P450, Family 1, Subfamily A, Polypeptide 1; Cytochrome P450 Form 6; Cytochrome P450-P1; Cytochrome P450-C; EC 1.14.14.1; CYPIA1; Cytochrome P1-450, Dioxin-Inducible; Flavoprotein-Linked Monooxygenase; Aryl Hydrocarbon Hydroxylase;
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301).

Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768).

Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997).

Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997).

Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462).

May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376).

May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).
Biological Process
9-cis-retinoic acid biosynthetic process Source: Ensembl
Aging Source: Ensembl
Amine metabolic process Source: Ensembl
Camera-type eye development Source: Ensembl
Cell population proliferation Source: Ensembl
Cellular response to copper ion Source: Ensembl
Cellular response to organic cyclic compound Source: MGI
Coumarin metabolic process Source: Ensembl
Dibenzo-p-dioxin catabolic process Source: Ensembl
Digestive tract development Source: Ensembl
DNA methylation Source: Ensembl
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: Reactome
Estrogen metabolic process Source: UniProtKB
Ethylene metabolic process Source: Reactome
Fatty acid metabolic process Source: UniProtKB
Flavonoid metabolic process Source: Ensembl
Hepatocyte differentiation Source: Ensembl
Hydrogen peroxide biosynthetic process Source: Ensembl
Insecticide metabolic process Source: Ensembl
Lipid hydroxylation Source: BHF-UCL
Long-chain fatty acid biosynthetic process Source: Reactome
Maternal process involved in parturition Source: Ensembl
Omega-hydroxylase P450 pathway Source: Reactome
Porphyrin-containing compound metabolic process Source: Ensembl
Positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
Regulation of metabolic process Source: Reactome
Response to antibiotic Source: Ensembl
Response to arsenic-containing substance Source: Ensembl
Response to drug Source: Ensembl
Response to food Source: Ensembl
Response to herbicide Source: Ensembl
Response to hyperoxia Source: Ensembl
Response to hypoxia Source: Ensembl
Response to immobilization stress Source: Ensembl
Response to iron(III) ion Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to nematode Source: Ensembl
Response to virus Source: Ensembl
Response to vitamin A Source: Ensembl
Response to wounding Source: Ensembl
Retinol metabolic process Source: UniProtKB
Steroid biosynthetic process Source: UniProtKB-KW
Steroid metabolic process Source: BHF-UCL
Vitamin D metabolic process Source: BHF-UCL
Cellular Location
Cytoplasm; Endoplasmic reticulum membrane; Microsome membrane; Mitochondrion inner membrane

Kyoreva, M., Li, Y., Hoosenally, M., Hardman-Smart, J., Morrison, K., Tosi, I., ... & Di Meglio, P. (2021). CYP1A1 enzymatic activity influences skin inflammation via regulation of the AHR pathway. Journal of Investigative Dermatology, 141(6), 1553-1563.

Santes-Palacios, R., Marroquín-Pérez, A. L., Hernández-Ojeda, S. L., Camacho-Carranza, R., Govezensky, T., & Espinosa-Aguirre, J. J. (2020). Human CYP1A1 inhibition by flavonoids. Toxicology in Vitro, 62, 104681.

Rannug, A. (2020). How the AHR became important in intestinal homeostasis—a diurnal FICZ/AHR/CYP1A1 feedback controls both immunity and immunopathology. International Journal of Molecular Sciences, 21(16), 5681.

Hoidy, W. H., Jaber, F. A., & Al-Askiry, M. A. (2019). Association of CYP1A1 rs1048943 polymorphism with prostate cancer in Iraqi men patients. Asian Pacific journal of cancer prevention: APJCP, 20(12), 3839.

Peris, P., González‐Roca, E., Rodríguez‐García, S. C., del Mar López‐Cobo, M., Monegal, A., & Guañabens, N. (2019). Incidence of mutations in the ALPL, GGPS1, and CYP1A1 genes in patients with atypical femoral fractures. JBMR plus, 3(1), 29-36.

Kapelyukh, Y., Henderson, C. J., Scheer, N., Rode, A., & Wolf, C. R. (2019). Defining the contribution of CYP1A1 and CYP1A2 to drug metabolism using humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 knockout mice. Drug Metabolism and Disposition, 47(8), 907-918.

Mescher, M., & Haarmann-Stemmann, T. (2018). Modulation of CYP1A1 metabolism: From adverse health effects to chemoprevention and therapeutic options. Pharmacology & Therapeutics, 187, 71-87.

Manzella, C., Singhal, M., Alrefai, W. A., Saksena, S., Dudeja, P. K., & Gill, R. K. (2018). Serotonin is an endogenous regulator of intestinal CYP1A1 via AhR. Scientific reports, 8(1), 1-13.

Sengupta, D., Guha, U., Mitra, S., Ghosh, S., Bhattacharjee, S., & Sengupta, M. (2018). Meta-analysis of polymorphic variants conferring genetic risk to cervical cancer in Indian women supports CYP1A1 as an important associated locus. Asian Pacific journal of cancer prevention: APJCP, 19(8), 2071.

Dai, Z. R., Feng, L., Jin, Q., Cheng, H., Li, Y., Ning, J., ... & Yang, L. (2017). A practical strategy to design and develop an isoform-specific fluorescent probe for a target enzyme: CYP1A1 as a case study. Chemical science, 8(4), 2795-2803.

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For research use only. Not intended for any clinical use.

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