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Mouse Anti-CYP1A2 Recombinant Antibody (D15) (CBMAB-C0614-CQ)

This product is a mouse antibody that recognizes CYP1A2. The antibody D15 can be used for immunoassay techniques such as: WB, ELISA, IHC, IF.
See all CYP1A2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D15
Antibody Isotype
IgG1
Application
WB, ELISA, IHC, IF

Basic Information

Immunogen
MC1a (Preparation C31B4, rat liver cytochrome P4501A2)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 1 Subfamily A Member 2
Introduction
CYP1A2 (Cytochrome P450 Family 1 Subfamily A Member 2) is a Protein Coding gene. Diseases associated with CYP1A2 include Toxicity Or Absent Response To Clozapine and Acetaminophen Metabolism. Among its related pathways are Gefitinib Pathway, Pharmacokinetics and Estrogen Receptor Pathway. Gene Ontology (GO) annotations related to this gene include enzyme binding and iron ion binding. An important paralog of this gene is CYP1A1.
Entrez Gene ID
Human1544
Mouse13077
Rat24297
UniProt ID
HumanP05177
MouseP00186
RatP04799
Alternative Names
Cytochrome P450 Family 1 Subfamily A Member 2; Cytochrome P450, Subfamily I (Aromatic Compound-Inducible), Polypeptide 2; Cytochrome P450, Family 1, Subfamily A, Polypeptide 2; Cholesterol 25-Hydroxylase; Cytochrome P(3)450; Cytochrome P450-P3; Cytochrome P450 4; EC 1.14.14.1; CYPIA2; Flavoprotein-Linked Monooxygenase;
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:9435160, PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:9435160, PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576).

Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:11555828, PubMed:12865317).

Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed:11555828, PubMed:12865317).

Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599).

May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376).

Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed:19965576).

Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed:9435160).

May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).

Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854).

Metabolizes caffeine via N3-demethylation (Probable).
Biological Process
Aflatoxin metabolic process Source: Reactome
Alkaloid metabolic process Source: BHF-UCL
Cellular respiration Source: Ensembl
Cellular response to cadmium ion Source: Ensembl
Cellular response to copper ion Source: Ensembl
Cholesterol metabolic process Source: UniProtKB-UniPathway
Dibenzo-p-dioxin metabolic process Source: Ensembl
Drug catabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: Reactome
Estrogen metabolic process Source: UniProtKB
Exogenous drug catabolic process Source: BHF-UCL
Heterocycle metabolic process Source: BHF-UCL
Hydrogen peroxide biosynthetic process Source: Ensembl
Long-chain fatty acid biosynthetic process Source: Reactome
Lung development Source: Ensembl
Methylation Source: Reactome
Monocarboxylic acid metabolic process Source: BHF-UCL
Monoterpenoid metabolic process Source: BHF-UCL
Omega-hydroxylase P450 pathway Source: Reactome
Oxidative demethylation Source: BHF-UCL
Porphyrin-containing compound metabolic process Source: Ensembl
Post-embryonic development Source: Ensembl
Regulation of gene expression Source: Ensembl
Response to estradiol Source: Ensembl
Response to immobilization stress Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Retinol metabolic process Source: UniProtKB
Steroid catabolic process Source: BHF-UCL
Toxin biosynthetic process Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane

Saha, A., Connick, J. P., Reed, J. R., Lott, C. S., & Backes, W. L. (2021). Identification of the contact region responsible for the formation of the homomeric CYP1A2• CYP1A2 complex. Biochemical Journal, 478(11), 2163-2178.

Grgic, J., Pickering, C., Del Coso, J., Schoenfeld, B. J., & Mikulic, P. (2021). CYP1A2 genotype and acute ergogenic effects of caffeine intake on exercise performance: A systematic review. European Journal of Nutrition, 60(3), 1181-1195.

Siokas, V., Karampinis, E., Aloizou, A. M., Mentis, A. F. A., Liakos, P., Papadimitriou, D., ... & Dardiotis, E. (2021). CYP1A2 rs762551 polymorphism and risk for amyotrophic lateral sclerosis. Neurological Sciences, 42(1), 175-182.

Siokas, V., Aloizou, A. M., Tsouris, Z., Liampas, I., Liakos, P., Calina, D., ... & Dardiotis, E. (2021). ADORA2A rs5760423 and CYP1A2 rs762551 polymorphisms as risk factors for Parkinson’s disease. Journal of clinical medicine, 10(3), 381.

Siokas, V., Kardaras, D., Aloizou, A. M., Liampas, I., Papageorgiou, E., Drakoulis, N., ... & Dardiotis, E. (2020). CYP1A2 rs762551 and ADORA2A rs5760423 Polymorphisms in Patients with Blepharospasm. Journal of Molecular Neuroscience, 70(9), 1370-1375.

Na Takuathung, M., Hanprasertpong, N., Teekachunhatean, S., & Koonrungsesomboon, N. (2019). Impact of CYP1A2 genetic polymorphisms on pharmacokinetics of antipsychotic drugs: a systematic review and meta‐analysis. Acta Psychiatrica Scandinavica, 139(1), 15-25.

Koonrungsesomboon, N., Khatsri, R., Wongchompoo, P., & Teekachunhatean, S. (2018). The impact of genetic polymorphisms on CYP1A2 activity in humans: a systematic review and meta-analysis. The pharmacogenomics journal, 18(6), 760-768.

Guest, N., Corey, P., Vescovi, J., & El-Sohemy, A. (2018). Caffeine, CYP1A2 genotype, and endurance performance in athletes. Medicine & Science in Sports & Exercise, 50(8), 1570-1578.

Puente, C., Abián-Vicén, J., Del Coso, J., Lara, B., & Salinero, J. J. (2018). The CYP1A2-163C> A polymorphism does not alter the effects of caffeine on basketball performance. PloS one, 13(4), e0195943.

Salinero, J. J., Lara, B., Ruiz-Vicente, D., Areces, F., Puente-Torres, C., Gallo-Salazar, C., ... & Del Coso, J. (2017). CYP1A2 genotype variations do not modify the benefits and drawbacks of caffeine during exercise: a pilot study. Nutrients, 9(3), 269.

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For research use only. Not intended for any clinical use.

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