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Mouse Anti-CYP2B6 Recombinant Antibody (3D5) (CBMAB-C5882-LY)

This product is antibody recognizes CYP2B6. The antibody 3D5 immunoassay techniques such as: FC, IF, IHC-P, WB.
See all CYP2B6 antibodies
Published Data
Fig.1 Cirrhotic liver sections from end-stage disease of patients with PFIC5 and PFIC2 were stained with anti-CYP2B6 antibody.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D5
Antibody Isotype
IgG2a
Application
FC, IF, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily B Member 6
Introduction
CYP2B6 (Cytochrome P450 Family 2 Subfamily B Member 6) is a Protein Coding gene. Diseases associated with CYP2B6 include Efavirenz, Poor Metabolism Of and Acute Frontal Sinusitis. Among its related pathways are Tamoxifen Pathway, Pharmacokinetics and Cytochrome P450 - arranged by substrate type. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen.
An important paralog of this gene is CYP2A13.
Entrez Gene ID
1555
UniProt ID
P20813
Alternative Names
Cytochrome P450 Family 2 Subfamily B Member 6; Cytochrome P450, Subfamily IIB (Phenobarbital-Inducible), Polypeptide 6; Cytochrome P450, Family 2, Subfamily B, Polypeptide 6; 1,4-Cineole 2-Exo-Monooxygenase; Cytochrome P450 IIB1; CYPIIB6; Cytochrome P450, Subfamily IIB (Phenobarbital-Inducible); Cytochrome P450, Family 2, Subfamily B; Cytochrome P450 2B6; EC 1.14.14.1;
Function
A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed:21289075, PubMed:12865317).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:21289075).

Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed:21289075, PubMed:12865317).

Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed:11695850, PubMed:22909231).

Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed:11695850).

Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.
Biological Process
Cellular ketone metabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
PTM
Phosphorylation is accompanied by a decrease in enzyme activity.

Mangó, K., Kiss, Á. F., Fekete, F., Erdős, R., & Monostory, K. (2022). CYP2B6 allelic variants and non-genetic factors influence CYP2B6 enzyme function. Scientific reports, 12(1), 1-14.

Desta, Z., El‐Boraie, A., Gong, L., Somogyi, A. A., Lauschke, V. M., Dandara, C., ... & Gaedigk, A. (2021). PharmVar GeneFocus: CYP2B6. Clinical Pharmacology & Therapeutics, 110(1), 82-97.

Griesel, R., Maartens, G., Chirehwa, M., Sokhela, S., Akpomiemie, G., Moorhouse, M., ... & Sinxadi, P. (2021). CYP2B6 genotype and weight gain differences between dolutegravir and efavirenz. Clinical Infectious Diseases, 73(11), e3902-e3909.

Langmia, I. M., Just, K. S., Yamoune, S., Brockmöller, J., Masimirembwa, C., & Stingl, J. C. (2021). CYP2B6 Functional Variability in Drug Metabolism and Exposure Across Populations—Implication for Drug Safety, Dosing, and Individualized Therapy. Frontiers in Genetics, 12, 1205.

Marok, F. Z., Fuhr, L. M., Hanke, N., Selzer, D., & Lehr, T. (2021). Physiologically based pharmacokinetic modeling of bupropion and its metabolites in a CYP2B6 drug-drug-gene interaction network. Pharmaceutics, 13(3), 331.

Helsby, N. A., Yong, M., van Kan, M., de Zoysa, J. R., & Burns, K. E. (2019). The importance of both CYP2C19 and CYP2B6 germline variations in cyclophosphamide pharmacokinetics and clinical outcomes. British Journal of Clinical Pharmacology, 85(9), 1925-1934.

Desta, Z., Gammal, R. S., Gong, L., Whirl‐Carrillo, M., Gaur, A. H., Sukasem, C., ... & Haas, D. W. (2019). Clinical pharmacogenetics implementation consortium (CPIC) guideline for CYP2B6 and efavirenz‐containing antiretroviral therapy. Clinical Pharmacology & Therapeutics, 106(4), 726-733.

Kharasch, E. D., & Crafford, A. (2019). Common polymorphisms of CYP2B6 influence stereoselective bupropion disposition. Clinical Pharmacology & Therapeutics, 105(1), 142-152.

Burgess, K. S., Ipe, J., Swart, M., Metzger, I. F., Lu, J., Gufford, B. T., ... & Skaar, T. C. (2018). Variants in the CYP2B6 3′ UTR alter in vitro and in vivo CYP2B6 activity: potential role of microRNAs. Clinical Pharmacology & Therapeutics, 104(1), 130-138.

Ahmad, T., Sabet, S., Primerano, D. A., Richards-Waugh, L. L., & Rankin, G. O. (2017). Tell-tale SNPs: the role of CYP2B6 in methadone fatalities. Journal of analytical toxicology, 41(4), 325-333.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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