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Recombinant Mouse Anti-CYP3A7 Recombinant Antibody (F19P2H2) (CBMAB-XB0324-YC)

Provided herein is a Mouse Recombinant Antibody against Cytochrome P450 Family 3 Subfamily A Member 7. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all CYP3A7 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
F19P2H2
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Ovalbumin-conjugated Synthetic peptide ESRDETVSGA (C-terminal sequence)
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 3 Subfamily A Member 7
Introduction
CYP3A7 (Cytochrome P450 Family 3 Subfamily A Member 7) is a protein coding gene. Among its related pathways are Metabolism and Cytochrome P450 - arranged by substrate type. Gene Ontology annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP3A7-CYP3A51P.
Entrez Gene ID
UniProt ID
Alternative Names
Cytochrome P450 Family 3 Subfamily A Member 7; Cytochrome P450, Family 3, Subfamily A, Polypeptide 7; Cytochrome P450, Subfamily IIIA, Polypeptide 7; Cytochrome P450-HFLA; EC 1.14.14.1; CYPIIIA7; Flavoprotein-Linked Monooxygenase; Aryl Hydrocarbon Hydroxylase;
Function
A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis (PubMed:9555064, PubMed:11093772, PubMed:14559847, PubMed:12865317, PubMed:17178770).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:9555064, PubMed:11093772, PubMed:14559847, PubMed:12865317, PubMed:17178770).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA), a precursor in the biosynthesis of androgen and estrogen steroid hormones (PubMed:9555064, PubMed:17178770).

Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1), particularly D-ring hydroxylated estrone at the C16-alpha position (PubMed:14559847, PubMed:12865317).

Mainly hydroxylates all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in atRA clearance during fetal development (PubMed:11093772).

Also involved in the oxidative metabolism of xenobiotics including anticonvulsants (PubMed:9555064).
Biological Process
Estrogen metabolic process Source: UniProtKB
Lipid hydroxylation Source: BHF-UCL
Oxidative demethylation Source: GO_Central
Retinoic acid metabolic process Source: UniProtKB
Retinol metabolic process Source: UniProtKB-UniPathway
Steroid biosynthetic process Source: UniProtKB-KW
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane

Dong, Y., Xu, Q., Li, R., Tao, Y., Zhang, Q., Li, J., ... & Qiu, X. (2022). CYP3A7, CYP3A4, and CYP3A5 genetic polymorphisms in recipients rather than donors influence tacrolimus concentrations in the early stages after liver transplantation. Gene, 809, 146007.

Shum, S., & Isoherranen, N. (2021). Human fetal liver metabolism of oxycodone is mediated by CYP3A7. The AAPS Journal, 23(1), 1-10.

Kandel, S. E., & Lampe, J. N. (2021). Inhibition of CYP3A7 DHEA-S oxidation by lopinavir and ritonavir: An alternative mechanism for adrenal impairment in HIV antiretroviral-treated neonates. Chemical research in toxicology, 34(4), 1150-1160.

Johnson, N., Maguire, S., Morra, A., Kapoor, P. M., Tomczyk, K., Jones, M. E., ... & Fletcher, O. (2021). CYP3A7* 1C allele: linking premenopausal oestrone and progesterone levels with risk of hormone receptor-positive breast cancers. British journal of cancer, 124(4), 842-854.

Work, H. M., Kandel, S. E., & Lampe, J. N. (2021). Characterization of fluorescent probe substrates to develop an efficient high-throughput assay for neonatal hepatic CYP3A7 inhibition screening. Scientific reports, 11(1), 1-13.

Okuyama, S., Kawamura, F., Kubiura, M., Tsuji, S., Osaki, M., Kugoh, H., ... & Tada, M. (2020). Real‐time fluorometric evaluation of hepatoblast proliferation in vivo and in vitro using the expression of CYP3A7 coding for human fetus‐specific P450. Pharmacology research & perspectives, 8(5), e00642.

Li, H., & Lampe, J. N. (2019). Neonatal cytochrome P450 CYP3A7: A comprehensive review of its role in development, disease, and xenobiotic metabolism. Archives of biochemistry and biophysics, 673, 108078.

Chen, Y. J., Zhang, J., Zhu, P. P., Tan, X. W., Lin, Q. H., Wang, W. X., ... & Lan, K. (2019). Stereoselective oxidation kinetics of deoxycholate in recombinant and microsomal CYP3A enzymes: deoxycholate 19-hydroxylation is an in vitro marker of CYP3A7 activity. Drug Metabolism and Disposition, 47(6), 574-581.

Sohn, M., Kim, M. G., Han, N., Kim, I. W., Gim, J., Min, S. I., ... & Oh, J. M. (2018). Whole exome sequencing for the identification of CYP3A7 variants associated with tacrolimus concentrations in kidney transplant patients. Scientific reports, 8(1), 1-10.

Kandel, S. E., Han, L. W., Mao, Q., & Lampe, J. N. (2017). Digging deeper into CYP3A testosterone metabolism: kinetic, regioselectivity, and stereoselectivity differences between CYP3A4/5 and CYP3A7. Drug Metabolism and Disposition, 45(12), 1266-1275.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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