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Mouse Anti-CYP46A1 Recombinant Antibody (N8-P6E4/H8) (CBMAB-1514-CN)

This product is a mouse antibody that recognizes CYP46A1 of human. The antibody N8-P6E4/H8 can be used for immunoassay techniques such as: WB, ELISA, IHC.
See all CYP46A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
N8-P6E4/H8
Antibody Isotype
IgG1
Application
WB, ELISA, IHC

Basic Information

Immunogen
Ovalbumin-conjugated synthetic peptide- PVLCTLRPR (C-terminal sequence)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
cytochrome P450, family 46, subfamily A, polypeptide 1
Introduction
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. This protein catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Entrez Gene ID
UniProt ID
Alternative Names
CP46; CYP46
Function
P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed:10377398, PubMed:14640697, PubMed:25017465, PubMed:18621681).

By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (By similarity).

Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (PubMed:12077124, PubMed:14640697, PubMed:28190002).

Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (PubMed:25017465).

May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (PubMed:20667828).
Biological Process
Bile acid biosynthetic process Source: Reactome
Cholesterol catabolic process Source: UniProtKB
Nervous system development Source: ProtInc
Progesterone metabolic process Source: UniProtKB
Protein localization to membrane raft Source: ARUK-UCL
Regulation of long-term synaptic potentiation Source: UniProtKB
Sterol metabolic process Source: Reactome
Xenobiotic metabolic process Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane; Postsynapse; Presynapse; Dendrite
Topology
Helical: 3-23

El‐Darzi, N., Mast, N., Petrov, A. M., & Pikuleva, I. A. (2021). 2‐Hydroxypropyl‐β‐cyclodextrin reduces retinal cholesterol in wild‐type and Cyp27a1−/− Cyp46a1−/− mice with deficiency in the oxysterol production. British journal of pharmacology, 178(16), 3220-3234.

Ali, T., Hannaoui, S., Nemani, S., Tahir, W., Zemlyankina, I., Cherry, P., ... & Gilch, S. (2021). Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice. Acta neuropathologica communications, 9(1), 1-14.

Han, M., Wang, S., Yang, N., Wang, X., Zhao, W., Saed, H. S., ... & Wang, J. (2020). Therapeutic implications of altered cholesterol homeostasis mediated by loss of CYP46A1 in human glioblastoma. EMBO molecular medicine, 12(1), e10924.

Petrov, A. M., Lam, M., Mast, N., Moon, J., Li, Y., Maxfield, E., & Pikuleva, I. A. (2019). CYP46A1 activation by efavirenz leads to behavioral improvement without significant changes in amyloid plaque load in the brain of 5XFAD mice. Neurotherapeutics, 16(3), 710-724.

Petrov, A. M., & Pikuleva, I. A. (2019). Cholesterol 24-hydroxylation by CYP46A1: benefits of modulation for brain diseases. Neurotherapeutics, 16(3), 635-648.

Kacher, R., Lamazière, A., Heck, N., Kappes, V., Mounier, C., Despres, G., ... & Betuing, S. (2019). CYP46A1 gene therapy deciphers the role of brain cholesterol metabolism in Huntington’s disease. Brain, 142(8), 2432-2450.

Theofilopoulos, S., de Oliveira, W. A. A., Yang, S., Yutuc, E., Saeed, A., Abdel-Khalik, J., ... & Arenas, E. (2019). 24 (S), 25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. Journal of Biological Chemistry, 294(11), 4169-4176.

Mast, N., Verwilst, P., Wilkey, C. J., Guengerich, F. P., & Pikuleva, I. A. (2019). In vitro activation of cytochrome P450 46A1 (CYP46A1) by efavirenz-related compounds. Journal of medicinal chemistry, 63(12), 6477-6488.

Mast, N., Anderson, K. W., Johnson, K. M., Phan, T. T., Guengerich, F. P., & Pikuleva, I. A. (2017). In vitro cytochrome P450 46A1 (CYP46A1) activation by neuroactive compounds. Journal of Biological Chemistry, 292(31), 12934-12946.

Mast, N., Lin, J. B., Anderson, K. W., Bjorkhem, I., & Pikuleva, I. A. (2017). Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1). PLoS One, 12(10), e0187168.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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