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Mouse Anti-CYP7A1 Recombinant Antibody (CBYY-C0730) (CBMAB-C2157-YY)

This product is mouse antibody that recognizes CYP7A1. The antibody CBYY-C0730 can be used for immunoassay techniques such as: ICC
See all CYP7A1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-C0730
Antibody Isotype
IgG1
Application
ICC

Basic Information

Immunogen
Human CYP7A1 synthetic peptide, Accession # P22680
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Protein A/G purified
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 7 Subfamily A Member 1
Entrez Gene ID
1581
UniProt ID
P22680
Alternative Names
Alanyl Aminopeptidase, Membrane; Microsomal Aminopeptidase; Aminopeptidase M; Myeloid Plasma Membrane Glycoprotein CD13; Alanyl (Membrane) Aminopeptidase; Membrane Alanyl Aminopeptidase; Aminopeptidase N; EC 3.4.11.2; GP150; CD13; PEPN;
Function
A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:2384150, PubMed:21813643).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:2384150, PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643).

Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150).

7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124).

Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643).
Biological Process
Bile acid and bile salt transport Source: Ensembl
Bile acid biosynthetic process Source: UniProtKB
Bile acid signaling pathway Source: Ensembl
Cellular response to cholesterol Source: UniProtKB
Cellular response to glucose stimulus Source: UniProtKB
Cholesterol catabolic process Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Negative regulation of collagen biosynthetic process Source: Ensembl
Negative regulation of fatty acid biosynthetic process Source: Ensembl
Positive regulation of cholesterol biosynthetic process Source: Ensembl
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of gene expression Source: Ensembl
Regulation of metabolic process Source: Reactome
Response to ethanol Source: Ensembl
Sterol metabolic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Topology
Helical: 4-24

Cao, K., Zhang, K., Ma, M., Ma, J., Tian, J., & Jin, Y. (2021). Lactobacillus mediates the expression of NPC1L1, CYP7A1, and ABCG5 genes to regulate cholesterol. Food Science & Nutrition, 9(12), 6882-6891.

Iwanicki, T., Balcerzyk, A., Niemiec, P., Trautsolt, W., Grzeszczak, W., Ochalska-Tyka, A., ... & Żak, I. (2019). The relationship between CYP7A1 polymorphisms, coronary artery disease & serum lipid markers. Biomarkers in Medicine, 13(14), 1199-1208.

Chambers, K. F., Day, P. E., Aboufarrag, H. T., & Kroon, P. A. (2019). Polyphenol effects on cholesterol metabolism via bile acid biosynthesis, CYP7A1: a review. Nutrients, 11(11), 2588.

Duan, Y., Zhang, F., Yuan, W., Wei, Y., Wei, M., Zhou, Y., ... & Wu, X. (2019). Hepatic cholesterol accumulation ascribed to the activation of ileum Fxr-Fgf15 pathway inhibiting hepatic Cyp7a1 in high-fat diet-induced obesity rats. Life sciences, 232, 116638.

Zhang, T., Zhao, M., Lu, D., Wang, S., Yu, F., Guo, L., ... & Wu, B. (2018). REV-ERBα regulates CYP7A1 through repression of liver receptor homolog-1. Drug Metabolism and Disposition, 46(3), 248-258.

Qayyum, F., Lauridsen, B. K., Frikke-Schmidt, R., Kofoed, K. F., Nordestgaard, B. G., & Tybjærg-Hansen, A. (2018). Genetic variants in CYP7A1 and risk of myocardial infarction and symptomatic gallstone disease. European Heart Journal, 39(22), 2106-2116.

Wang, D., Hartmann, K., Seweryn, M., & Sadee, W. (2018). Interactions between regulatory variants in CYP7A1 (cholesterol 7α-hydroxylase) promoter and enhancer regions regulate CYP7A1 expression. Circulation: Genomic and Precision Medicine, 11(10), e002082.

Enya, S., Kawakami, K., Suzuki, Y., & Kawaoka, S. (2018). A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor–liver crosstalk in causing adverse effects on the host. Disease models & mechanisms, 11(8), dmm032383.

Abdullah, M. M., Eck, P. K., Couture, P., Lamarche, B., & Jones, P. J. (2018). The combination of single nucleotide polymorphisms rs6720173 (ABCG5), rs3808607 (CYP7A1), and rs760241 (DHCR7) is associated with differing serum cholesterol responses to dairy consumption. Applied Physiology, Nutrition, and Metabolism, 43(10), 1090-1093.

Wang, Y., Ding, Y., Li, J., Chavan, H., Matye, D., Ni, H. M., ... & Li, T. (2017). Targeting the enterohepatic bile acid signaling induces hepatic autophagy via a CYP7A1–AKT–mTOR axis in mice. Cellular and molecular gastroenterology and hepatology, 3(2), 245-260.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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