Mouse Anti-DALRD3 Recombinant Antibody (H-12) (CBMAB-D0184-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DALR Anticodon Binding Domain Containing 3 (DALRD3). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all DALRD3 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

H-12

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DALR anticodon binding domain containing 3

Introduction

DALRD3 is a protein with a DALR anticodon binding domain similar to that of class Ia aminoacyl tRNA synthetases. This gene is located in a cluster of genes (with a complex sense-anti-sense genome architecture) on chromosome 3, and contains two micro RNA (miRNA) precursors (mir-425 and mir-191) in one of its introns. Preferential expression of this gene (the miRNAs and other genes in the cluster) in testis suggests a role of this gene in spermatogenesis.

Entrez Gene ID

Human	55152
Mouse	67789
Rat	363146

UniProt ID

Human	Q5D0E6
Mouse	Q6PJN8
Rat	Q641Y9

Alternative Names

DALR Anticodon Binding Domain Containing 3;

Function

Involved in tRNA methylation. Facilitates the recognition and targeting of tRNA(Arg)(CCU) and tRNA(Arg)(UCU) substrates for N3-methylcytidine modification by METTL2A and METTL2B.

Biological Process

Arginyl-tRNA aminoacylation Source: InterPro
tRNA C3-cytosine methylation Source: UniProtKB

Involvement in disease

Developmental and epileptic encephalopathy 86 (DEE86):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE86 inheritance is autosomal recessive.

References

Lentini, J. M., Alsaif, H. S., Faqeih, E., Alkuraya, F. S., & Fu, D. (2020). DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification. Nature communications, 11(1), 1-14.

Lentini, J. M., & Fu, D. (2019). METTL2 forms a complex with the DALRD3 anticodon-domain binding protein to catalyze formation of 3-methylcytosine in specific arginine tRNA isoacceptors. bioRxiv, 745240.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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