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Rat Anti-DAND5 Recombinant Antibody (CBYCD-538) (CBMAB-D0194-YC)

Provided herein is a Rat monoclonal antibody, which binds to DAN Domain BMP Antagonist Family Member 5 (DAND5). The antibody can be used for immunoassay techniques, such as WB.
See all DAND5 antibodies

Summary

Host Animal
Rat
Specificity
Mouse
Clone
CBYCD-538
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
E. coli-derived recombinant mouse COCO Arg24-Leu185
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DAN Domain BMP Antagonist Family Member 5
Introduction
DAND5 belongs to the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry.
Entrez Gene ID
UniProt ID
Alternative Names
DAN Domain BMP Antagonist Family Member 5; Cysteine Knot Superfamily 1, BMP Antagonist 3; DAN Domain Family Member 5, BMP Antagonist; Cerberus-Like Protein 2; Gremlin-3; CKTSF1B3; Cerl-2; GREM3; CER2; SP1;
Function
Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation (By similarity).
Biological Process
Atrial septum development Source: BHF-UCL
Determination of heart left/right asymmetry Source: BHF-UCL
Determination of left/right asymmetry in lateral mesoderm Source: BHF-UCL
Negative regulation of BMP signaling pathway Source: BHF-UCL
Negative regulation of nodal signaling pathway Source: BHF-UCL
Negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry Source: GO_Central
Negative regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
Sequestering of BMP in extracellular matrix Source: GO_Central
Sequestering of nodal from receptor via nodal binding Source: BHF-UCL
Signal transduction involved in regulation of gene expression Source: GO_Central
Ventricular septum development Source: BHF-UCL
Cellular Location
Secreted

Popovic, N., Hooker, E., Barabino, A., Flamier, A., Provost, F., Buscarlet, M., ... & Larrivée, B. (2021). COCO/DAND5 inhibits developmental and pathological ocular angiogenesis. EMBO molecular medicine, 13(3), e12005.

Minegishi, K., Rothé, B., Komatsu, K. R., Ono, H., Ikawa, Y., Nishimura, H., ... & Hamada, H. (2021). Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex. Nature Communications, 12(1), 1-17.

Maerker, M., Getwan, M., Dowdle, M. E., McSheene, J. C., Gonzalez, V., Pelliccia, J. L., ... & Schweickert, A. (2021). Bicc1 and Dicer regulate left-right patterning through post-transcriptional control of the Nodal inhibitor Dand5. Nature communications, 12(1), 1-15.

Jacinto, R., Sampaio, P., Roxo-Rosa, M., Pestana, S., & Lopes, S. S. (2021). Pkd2 Affects Cilia Length and Impacts LR Flow Dynamics and Dand5. Frontiers in Cell and Developmental Biology, 9, 756.

Inácio, J. M., von Gilsa Lopes, J., Silva, A. M., Cristo, F., Marques, S., Futschik, M. E., & Belo, J. A. (2021). DAND5 Inactivation Enhances Cardiac Differentiation in Mouse Embryonic Stem Cells. Frontiers in cell and developmental biology, 9, 846.

Zhu, X., Shi, C., Zhong, Y., Liu, X., Yan, Q., Wu, X., ... & Li, G. (2020). Cilia-driven asymmetric Hedgehog signalling determines the amphioxus left-right axis by controlling Dand5 expression. Development, 147(1), dev182469.

Inácio, J. M., Almeida, M., Cristo, F., & Belo, J. A. (2020). Generation of a gene-corrected human induced pluripotent stem cell line derived from a patient with laterality defects and congenital heart anomalies with a c. 455G> A alteration in DAND5. Stem Cell Research, 42, 101677.

Miao, X., Zhang, Y., Sun, J., Cui, S., Meng, Q., Zhu, K., ... & Wang, T. (2017). Elevated serum DAND5 is associated with metastasis and predicts poor prognosis in colorectal cancer. United European Gastroenterology Journal, 5(5), 725-734.

Cristo, F., Inacio, J. M., Rosas, G., Carreira, I. M., Melo, J. B., de Almeida, L. P., ... & Belo, J. A. (2017). Generation of human iPSC line from a patient with laterality defects and associated congenital heart anomalies carrying a DAND5 missense alteration. Stem Cell Research, 25, 152-156.

Cristo, F., Inácio, J. M., de Almeida, S., Mendes, P., Martins, D. S., Maio, J., ... & Belo, J. A. (2017). Functional study of DAND5 variant in patients with congenital heart disease and laterality defects. BMC Medical Genetics, 18(1), 1-9.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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