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Rabbit Anti-DBH Monoclonal Antibody (EPR20385) (CBMAB-1043-YC)

Provided herein is a rabbit monoclonal antibody against Human DotH. The antibody, clone EPR20385, can be used for immunoassay techniques, such as IHC-P, IP and WB.
See all DBH antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EPR20385
Antibody Isotype
IgG
Application
IHC-P, IP, WB

Basic Information

Immunogen
Recombinant fragment within Human Dopamine beta Hydroxylase aa 50-350.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Dopamine Beta-Hydroxylase
Introduction
Dopamine beta-hydroxylase catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Dopamine beta-hydroxylate deficiency in human patients characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis and polymorphisms may play a role in a variety of psychiatric disorders.
Entrez Gene ID
Human1621
Mouse13166
Rat25699
UniProt ID
HumanP09172
MouseQ64237
RatQ05754
Alternative Names
DBM
Function
Conversion of dopamine to noradrenaline.
Biological Process
Behavioral response to ethanol Source: Ensembl
Blood vessel remodeling Source: Ensembl
Catecholamine biosynthetic process Source: Reactome
Chemical synaptic transmission Source: ProtInc
Dopamine catabolic process Source: UniProtKB
Fear response Source: Ensembl
Glucose homeostasis Source: Ensembl
Homoiothermy Source: Ensembl
Leukocyte mediated immunity Source: Ensembl
Leukocyte migration Source: Ensembl
Locomotory behavior Source: Ensembl
Maternal behavior Source: Ensembl
Memory Source: Ensembl
Norepinephrine biosynthetic process Source: UniProtKB
Octopamine biosynthetic process Source: GO_Central
Positive regulation of cold-induced thermogenesis Source: YuBioLab
Positive regulation of vasoconstriction Source: Ensembl
Regulation of cell population proliferation Source: Ensembl
Regulation of extrinsic apoptotic signaling pathway Source: Ensembl
Response to amphetamine Source: Ensembl
Response to pain Source: Ensembl
Visual learning Source: Ensembl
Cellular Location
Soluble dopamine beta-hydroxylase: Secreted; Secretory vesicle lumen; Chromaffin granule lumen; Secretory vesicle membrane; Chromaffin granule membrane
Involvement in disease
Orthostatic hypotension 1 (ORTHYP1):
A form of orthostatic hypotension due to congenital dopamine beta-hydroxylase deficiency. Orthostatic hypotension, also known as postural hypotension, is a finding defined as a 20-mm Hg decrease in systolic pressure or a 10-mm Hg decrease in diastolic pressure occurring 3 minutes after a person has risen from supine to standing. Symptoms include dizziness, blurred vision, and sometimes syncope. ORTHYP1 is an autosomal recessive condition apparent from infancy or early childhood and characterized by low plasma and urinary levels of norepinephrine and epinephrine, and episodic hypoglycemia.
Topology
Cytoplasmic: 1-176
Helical: 17-37
Intragranular: 38-617
PTM
N-glycosylated.
Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form.

Hobgood, D. K. (2021). ABO B gene is associated with introversion personality tendancies through linkage with dopamine beta hydroxylase gene. Medical Hypotheses, 148, 110513.

Polasik, D., Konieczna, A., Terman, A., & Dybus, A. (2021). The association of C789A polymorphism in the dopamine beta-hydroxylase gene (DBH) and aggressive behaviour in dogs. Acta Veterinaria Brno, 90(3), 295-299.

Wassenberg, T., Deinum, J., van Ittersum, F. J., Kamsteeg, E. J., Pennings, M., Verbeek, M. M., ... & Willemsen, M. A. (2021). Clinical presentation and long‐term follow‐up of dopamine beta hydroxylase deficiency. Journal of inherited metabolic disease, 44(3), 554-565.

Kundu, S., Saini, M., Dey, S. K., & Kundu, S. (2020). Dopamine Beta Hydroxylase: An Enzyme with Therapeutic Potential to Combat Neural and Cardiovascular Diseases. In Frontiers in Protein Structure, Function, and Dynamics (pp. 339-357). Springer, Singapore.

Barbanti, P., Guadagni, F., De Marchis, M. L., Ialongo, C., Egeo, G., Fofi, L., ... & Palmirotta, R. (2019). Dopamine-beta-hydroxylase 19-bp insertion/deletion polymorphism affects medication overuse in patients with chronic migraine. Neurological Sciences, 40(8), 1717-1724.

Shalev, N., Vangkilde, S., Neville, M. J., Tunbridge, E. M., Nobre, A. C., & Chechlacz, M. (2019). Dissociable catecholaminergic modulation of visual attention: differential effects of catechol-O-methyltransferase and dopamine beta-hydroxylase genes on visual attention. Neuroscience, 412, 175-189.

Tang, S., Yao, B., Li, N., Lin, S., & Huang, Z. (2018). Association of dopamine beta-hydroxylase polymorphisms with alzheimer’s disease, Parkinson’s disease and schizophrenia: evidence based on currently available loci. Cellular Physiology and Biochemistry, 51(1), 411-428.

Yamaguchi, H., Hopf, F. W., Li, S. B., & de Lecea, L. (2018). In vivo cell type-specific CRISPR knockdown of dopamine beta hydroxylase reduces locus coeruleus evoked wakefulness. Nature communications, 9(1), 1-8.

Cheng, W., Ka, Y. W., & Chang, C. C. (2017). Involvement of dopamine beta-hydroxylase in the neuroendocrine-immune regulatory network of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 68, 92-101.

Bozek, T., Blazekovic, A., Perkovic, M. N., Jercic, K. G., Sustar, A., Smircic-Duvnjak, L., ... & Borovecki, F. (2017). The influence of dopamine-beta-hydroxylase and catechol O-methyltransferase gene polymorphism on the efficacy of insulin detemir therapy in patients with type 2 diabetes mellitus. Diabetology & metabolic syndrome, 9(1), 1-11.

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For research use only. Not intended for any clinical use.

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