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Mouse Anti-DBR1 Recombinant Antibody (3A7) (CBMAB-D0314-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Debranching RNA Lariats 1 (DBR1). The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all DBR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3A7
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
DBR1 (NP_057300.2, 445 a.a. ~ 538 a.a) partial recombinant protein with GST tag. The immunogen sequence: SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDD
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 445-538

Target

Full Name
debranching enzyme homolog 1 (S. cerevisiae)
Introduction
DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication.
Entrez Gene ID
51163
UniProt ID
Q9UK59
Alternative Names
Debranching RNA Lariats 1; Debranching Enzyme (S. Cerevisiae) Homolog 1; Debranching Enzyme Homolog 1 (S. Cerevisiae); EC 3.1.-.-;
Function
Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis.
Biological Process
mRNA splicing, via spliceosome Source: GO_Central
RNA splicing, via transesterification reactions Source: UniProtKB
Cellular Location
Nucleus

Xu, X., Yang, X., Liu, X., Bi, Y., Kong, P., Wang, Y., ... & Xi, Y. (2022). The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma. Technology in Cancer Research & Treatment, 21, 15330338221083105.

Mohanta, A., & Chakrabarti, K. (2021). Dbr1 functions in mRNA processing, intron turnover and human diseases. Biochimie, 180, 134-142.

Valdés, J., Ortuño-Pineda, C., Saucedo-Cárdenas, O., & Mendoza-Figueroa, M. S. (2018). Unexplored Molecular Features of the Entamoeba histolytica RNA Lariat Debranching Enzyme Dbr1 Expression Profile. Frontiers in Cellular and Infection Microbiology, 8, 228.

Katolik, A., Clark, N. E., Tago, N., Montemayor, E. J., Hart, P. J., & Damha, M. J. (2017). Fluorescent Branched RNAs for High-Throughput Analysis of Dbr1 Enzyme Kinetics and Inhibition. ACS chemical biology, 12(3), 622-627.

Han, B., Park, H. K., Ching, T., Panneerselvam, J., Wang, H., Shen, Y., ... & Fei, P. (2017). Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. Oncogene, 36(38), 5382-5391.

Galvis, A. E., Fisher, H. E., Fan, H., & Camerini, D. (2017). Conformational changes in the 5′ end of the HIV-1 genome dependent on the debranching enzyme DBR1 during early stages of infection. Journal of Virology, 91(23), e01377-17.

Ransey, E., Paredes, E., Dey, S. K., Das, S. R., Heroux, A., & Macbeth, M. R. (2017). Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn2+/Mn2+ heterobinucleation. FEBS letters, 591(13), 2003-2010.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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