Mouse Anti-DCST1 Recombinant Antibody (2C12) (CBMAB-D0396-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DC-STAMP Domain Containing 1 (DCST1). The antibody can be used for immunoassay techniques, such as ELISA.
See all DCST1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2C12

Antibody Isotype

IgG2a, κ

Application

ELISA

Basic Information

Immunogen

DCST1 (NP_689707, 532 a.a. ~ 630 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DC-STAMP domain containing 1

Introduction

DCST1 is a protein with a domain similar to one found in dendritic cells which play a key role in antigen processing and display for immune responses.

Entrez Gene ID

149095

UniProt ID

Q5T197

Alternative Names

DC-STAMP Domain Containing 1; DC-STAMP Domain-Containing Protein 1; RING-Type E3 Ubiquitin Transferase; EC 2.3.2.27;

Function

E3 ubiquitin-protein ligase which mediates 'Lys-48'-linked ubiquitination of STAT2 and induces its proteosomal degradation thereby negatively regulating type-I-interferon signaling.

Biological Process

Innate immune response Source: UniProtKB-KW
Negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-48
Helical: 49-69
Extracellular: 70-79
Helical: 80-100
Cytoplasmic: 101-115
Helical: 116-136
Extracellular: 137-372
Helical: 373-393
Cytoplasmic: 394-463
Helical: 464-484
Extracellular: 485-553
Helical: 554-574
Cytoplasmic: 575-706

References

Noda, T., Blaha, A., Fujihara, Y., Gert, K. R., Emori, C., Deneke, V. E., ... & Ikawa, M. (2022). Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Communications Biology, 5(1), 1-11.

Inoue, N., Hagihara, Y., & Wada, I. (2021). Evolutionarily conserved sperm factors, DCST1 and DCST2, are required for gamete fusion. Elife, 10, e66313.

Wang, J., Lei, C., Shi, P., Teng, H., Lu, L., Guo, H., & Wang, X. (2021). LncRNA DCST1-AS1 Promotes Endometrial Cancer Progression by Modulating the MiR-665/HOXB5 and MiR-873-5p/CADM1 Pathways. Frontiers in Oncology, 11.

Tang, L., Chen, Y., Tang, X., Wei, D., Xu, X., & Yan, F. (2020). Long noncoding RNA DCST1-AS1 promotes cell proliferation and metastasis in triple-negative breast cancer by forming a positive regulatory loop with miR-873-5p and MYC. Journal of Cancer, 11(2), 311.

Tang, L., Chen, Y., Chen, H., Jiang, P., Yan, L., Mo, D., ... & Yan, F. (2020). DCST1-AS1 Promotes TGF-β-Induced Epithelial–Mesenchymal Transition and Enhances Chemoresistance in Triple-Negative Breast Cancer Cells via ANXA1. Frontiers in oncology, 10, 280.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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