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Mouse Anti-DCUN1D1 Recombinant Antibody (4B5) (CBMAB-D0424-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Defective In Cullin Neddylation 1 Domain Containing 1 (DCUN1D1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DCUN1D1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4B5
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
DCUN1D1 (NP_065691, 1 a.a. ~ 89 a.a) partial recombinant protein with GST tag. The immunogen sequence: MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL YIRESVKGSL DRKKLEQLYN RYKDPQDENK IGIDGIQQF
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-89

Target

Full Name
DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
Introduction
DCUN1D1 (Defective In Cullin Neddylation 1 Domain Containing 1) is a protein coding gene. Diseases associated with DCUN1D1 include Squamous Cell Carcinoma Of The Oral Tongue and Squamous Cell Carcinoma. And a paralog is MCCC1.
Entrez Gene ID
54165
UniProt ID
Q96GG9
Alternative Names
Defective In Cullin Neddylation 1 Domain Containing 1; Defective In Cullin Neddylation Protein 1-Like Protein 1; Squamous Cell Carcinoma-Related Oncogene; DCUN1 Domain-Containing Protein 1; DCUN1L1; SCCRO; RP42; DCN1, Defective In Cullin Neddylation 1, Domain Containing 1 (S. Cerevisiae);
Function
Part of an E3 ubiquitin ligase complex for neddylation (PubMed:18826954).

Promotes neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes (PubMed:26906416, PubMed:23201271, PubMed:19617556, PubMed:23401859).

Acts by binding to cullin-RBX1 complexes in the cytoplasm and promoting their nuclear translocation, enhancing recruitment of E2-NEDD8 (UBE2M-NEDD8) thioester to the complex, and optimizing the orientation of proteins in the complex to allow efficient transfer of NEDD8 from the E2 to the cullin substrates. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity (PubMed:25349211, PubMed:28581483).

Acts also as an oncogene facilitating malignant transformation and carcinogenic progression (By similarity).
Biological Process
Positive regulation of protein neddylation Source: UniProtKB
Positive regulation of ubiquitin-protein transferase activity Source: GO_Central
Post-translational protein modification Source: Reactome
Protein neddylation Source: GO_Central
Regulation of protein neddylation Source: UniProtKB
Regulation of protein ubiquitination Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. The ubiquitinated form is localized in the cytoplasm.
PTM
Mono- and poly-ubiquitinated by ARIH2 and ARIH1. Monoubiquitination by ARIH2 is mediated by an interaction between autoubiquitinated ARIH2 and the UBA-like domain. The monoubiquitinated form preferentially interacts with non-neddylated cullins and modulates cullin RING ligase (CRL) complex composition and activity.

Huang, S. T., Liu, Z. Z., Jiang, F. N., He, H. C., & Zhong, W. D. (2021). DCUN1D1 promotes tumor progress in prostate cancer and its effect on DU145 in vitro. Journal of the Pakistan Medical Association, 71(2), 473-478.

Li, J., Yu, T., Yan, M., Zhang, X., Liao, L., Zhu, M., ... & Yao, M. (2019). DCUN1D1 facilitates tumor metastasis by activating FAK signaling and up-regulates PD-L1 in non-small-cell lung cancer. Experimental Cell Research, 374(2), 304-314.

Huang, G., Kaufman, A. J., Ryan, R. J., Romin, Y., Huryn, L., Bains, S., ... & Singh, B. (2019). Mouse DCUN1D1 (SCCRO) is required for spermatogenetic individualization. Plos one, 14(1), e0209995.

Xiao, J., Li, G., Zhou, J., Wang, S., Liu, D., Shu, G., ... & Ren, F. (2018). MicroRNA-520b functions as a tumor suppressor in colorectal cancer by inhibiting defective in cullin neddylation 1 domain containing 1 (DCUN1D1). Oncology research, 26(4), 593.

Zhong, J., Yuan, H., Xu, X., & Kong, S. (2018). MicroRNA‑195 inhibits cell proliferation, migration and invasion by targeting defective in cullin neddylation 1 domain containing 1 in cervical cancer Retraction in/10.3892/ijmm. 2021.4973. International Journal of Molecular Medicine, 42(2), 779-788.

Jiang, Y., Hou, R., Li, S., Li, S., & Dang, G. (2018). MicroRNA‑302 inhibits cell migration and invasion in cervical cancer by targeting DCUN1D1. Experimental and therapeutic medicine, 16(2), 1000-1008.

Zhang, Z. H., Li, J., Luo, F., & Wang, Y. S. (2017). Clinical significance of SCCRO (DCUN1D1) in prostate cancer and its proliferation-inhibiting effect on Lncap cells. Eur Rev Med Pharmacol Sci, 21(19), 4283-4291.

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For research use only. Not intended for any clinical use.

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