Sign in or Register   Sign in or Register
  |  

Mouse Anti-DFFA Recombinant Antibody (AF41E3) (CBMAB-I1882-YY)

This product is Mouse antibody that recognizes DFFA. The antibody AF41E3 can be used for immunoassay techniques such as: ELISA, IP
See all DFFA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
AF41E3
Antibody Isotype
IgG2a, κ
Application
ELISA, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES buffer with 0.15M NaCl, 0.01% BSA, 50% glycerol
Preservative
.03% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Fragmentation Factor Subunit Alpha
Introduction
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Entrez Gene ID
1676
UniProt ID
O00273
Alternative Names
DNA Fragmentation Factor Subunit Alpha; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45 KDa Subunit; Inhibitor Of CAD; DFF-45; DFF45;
Function
Inhibitor of the caspase-activated DNase (DFF40).
Biological Process
Apoptotic DNA fragmentation Source: Reactome
Chaperone-mediated protein folding Source: CAFA
Negative regulation of apoptotic DNA fragmentation Source: MGI
Negative regulation of deoxyribonuclease activity Source: CAFA
Negative regulation of execution phase of apoptosis Source: BHF-UCL
Positive regulation of apoptotic process Source: Ensembl
Regulation of apoptotic process Source: GO_Central
Thymocyte apoptotic process Source: Ensembl
Cellular Location
Cytoplasm
PTM
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DFFA Recombinant Antibody (AF41E3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare