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Mouse Anti-DHFR Recombinant Antibody (CBYCD-259) (CBMAB-D0822-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dihydrofolate Reductase (DHFR). The antibody can be used for immunoassay techniques, such as WB, IHC, ICC.
See all DHFR antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-259
Antibody Isotype
IgG2b
Application
WB, IHC, ICC

Basic Information

Immunogen
E. coli-derived recombinant human Dihydrofolate Reductase/DHFR, Met1-Asp187, Accession # P00374
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dihydrofolate reductase
Introduction
DHFR converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. DHFR deficiency has been linked to megaloblastic anemia.
Entrez Gene ID
Human1719
Mouse13361
Rat24312
UniProt ID
HumanP00374
MouseP00375
RatQ920D2
Alternative Names
Dihydrofolate Reductase; EC 1.5.1.3; DHFRP1; DYR;
Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Biological Process
Axon regeneration Source: BHF-UCL
Dihydrofolate metabolic process Source: BHF-UCL
Folic acid metabolic process Source: BHF-UCL
Glycine biosynthetic process Source: InterPro
Negative regulation of translation Source: CAFA
One-carbon metabolic process Source: UniProtKB-KW
Positive regulation of nitric-oxide synthase activity Source: BHF-UCL
Regulation of removal of superoxide radicals Source: BHF-UCL
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Response to methotrexate Source: BHF-UCL
Tetrahydrobiopterin biosynthetic process Source: BHF-UCL
Tetrahydrofolate biosynthetic process Source: BHF-UCL
Tetrahydrofolate metabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Mitochondrion
Involvement in disease
Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD):
An inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms.

Huang, K., Narumi, T., Zhang, Y., Li, Q., Murugesan, P., Wu, Y., ... & Cai, H. (2021). Targeting MicroRNA-192-5p, a downstream effector of NOXs (NADPH oxidases), reverses endothelial DHFR (dihydrofolate reductase) deficiency to attenuate abdominal aortic aneurysm formation. Hypertension, 78(2), 282-293.

Ammar, Y. A., Abd El-Hafez, S. M., Hessein, S. A., Ali, A. M., Askar, A. A., & Ragab, A. (2021). One-pot strategy for thiazole tethered 7-ethoxy quinoline hybrids: Synthesis and potential antimicrobial agents as dihydrofolate reductase (DHFR) inhibitors with molecular docking study. Journal of Molecular Structure, 1242, 130748.

Wróbel, A., Baradyn, M., Ratkiewicz, A., & Drozdowska, D. (2021). Synthesis, biological activity, and molecular dynamics study of novel series of a trimethoprim analogs as multi-targeted compounds: Dihydrofolate reductase (DHFR) inhibitors and DNA-binding agents. International journal of molecular sciences, 22(7), 3685.

He, J., Qiao, W., An, Q., Yang, T., & Luo, Y. (2020). Dihydrofolate reductase inhibitors for use as antimicrobial agents. European Journal of Medicinal Chemistry, 195, 112268.

Li, H., Li, Q., Zhang, Y., Liu, W., Gu, B., Narumi, T., ... & Cai, H. (2019). Novel treatment of hypertension by specifically targeting E2F for restoration of endothelial dihydrofolate reductase and eNOS function under oxidative stress. Hypertension, 73(1), 179-189.

Teixeira, B. V. F., Teles, A. L. B., Silva, S. G. D., Brito, C. C. B., Freitas, H. F. D., Pires, A. B. L., ... & Castilho, M. S. (2019). Dual and selective inhibitors of pteridine reductase 1 (PTR1) and dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Leishmania chagasi. Journal of enzyme inhibition and medicinal chemistry, 34(1), 1439-1450.

Hussein, E. M., Al-Rooqi, M. M., Abd El-Galil, S. M., & Ahmed, S. A. (2019). Design, synthesis, and biological evaluation of novel N 4-substituted sulfonamides: acetamides derivatives as dihydrofolate reductase (DHFR) inhibitors. BMC chemistry, 13(1), 1-18.

Francesconi, V., Giovannini, L., Santucci, M., Cichero, E., Costi, M. P., Naesens, L., ... & Tonelli, M. (2018). Synthesis, biological evaluation and molecular modeling of novel azaspiro dihydrotriazines as influenza virus inhibitors targeting the host factor dihydrofolate reductase (DHFR). European journal of medicinal chemistry, 155, 229-243.

Nakano, M., Fukami, T., Gotoh, S., & Nakajima, M. (2017). A-to-I RNA editing up-regulates human dihydrofolate reductase in breast cancer. Journal of Biological Chemistry, 292(12), 4873-4884.

Tonelli, M., Naesens, L., Gazzarrini, S., Santucci, M., Cichero, E., Tasso, B., ... & Loddo, R. (2017). Host dihydrofolate reductase (DHFR)-directed cycloguanil analogues endowed with activity against influenza virus and respiratory syncytial virus. European journal of medicinal chemistry, 135, 467-478.

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For research use only. Not intended for any clinical use.

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