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Mouse Anti-DMC1 Recombinant Antibody (5B10/2) (CBMAB-AP1905LY)


See all DMC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5B10/2
Antibody Isotype
IgG2a
Application
IHC, IP, WB

Basic Information

Immunogen
Recombinant fusion protein (Human). DMC1 protein (expressed as 6xHis fusion in E. coli and band cut out of gel of insoluble pellet).
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DNA Meiotic Recombinase 1
Introduction
This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Entrez Gene ID
11144
UniProt ID
Q14565
Alternative Names
DNA Meiotic Recombinase 1; DMC1H; LIM15; DMC1 (Dosage Suppressor Of Mck1, Yeast Homolog) Meiosis-Specific Homologous Recombination; DMC1 Dosage Suppressor Of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast); DMC1 Dosage Suppressor Of Mck1 Homolog, Meiosis-Specific Homologous Recombination; Meiotic Recombination Protein DMC1/LIM15 Homolog; Disrupted Meiotic CDNA1, Yeast, Homolog Of; DJ199H16.1;
Function
Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Biological Process
Chromosome organization involved in meiotic cell cycle Source: GO_Central
DNA recombinase assembly Source: GO_Central
Female gamete generation Source: ProtInc
Homologous chromosome pairing at meiosis Source: Ensembl
Male meiosis I Source: Ensembl
Meiotic cell cycle Source: ProtInc
Mitotic recombination Source: GO_Central
Oocyte maturation Source: Ensembl
Ovarian follicle development Source: Ensembl
Reciprocal meiotic recombination Source: UniProtKB
Spermatid development Source: Ensembl
Spermatogenesis Source: ProtInc
Strand invasion Source: GO_Central
Cellular Location
Nucleus; Chromosome

Takemoto, K., Tani, N., Takada-Horisawa, Y., Fujimura, S., Tanno, N., Yamane, M., ... & Ishiguro, K. I. (2020). Meiosis-specific C19orf57/4930432K21Rik/BRME1 modulates localization of RAD51 and DMC1 to DSBs in mouse meiotic recombination. Cell reports, 31(8), 107686.

Lan, W. H., Lin, S. Y., Kao, C. Y., Chang, W. H., Yeh, H. Y., Chang, H. Y., ... & Li, H. W. (2020). Rad51 facilitates filament assembly of meiosis-specific Dmc1 recombinase. Proceedings of the National Academy of Sciences, 117(21), 11257-11264.

Slotman, J. A., Paul, M. W., Carofiglio, F., de Gruiter, H. M., Vergroesen, T., Koornneef, L., ... & Baarends, W. M. (2020). Super-resolution imaging of RAD51 and DMC1 in DNA repair foci reveals dynamic distribution patterns in meiotic prophase. PLoS genetics, 16(6), e1008595.

Hinch, A. G., Becker, P. W., Li, T., Moralli, D., Zhang, G., Bycroft, C., ... & Donnelly, P. (2020). The configuration of RPA, RAD51, and DMC1 binding in meiosis reveals the nature of critical recombination intermediates. Molecular cell, 79(4), 689-701.

Steinfeld, J. B., Beláň, O., Kwon, Y., Terakawa, T., Al-Zain, A., Smith, M. J., ... & Greene, E. C. (2019). Defining the influence of Rad51 and Dmc1 lineage-specific amino acids on genetic recombination. Genes & development, 33(17-18), 1191-1207.

Chan, Y. L., Zhang, A., Weissman, B. P., & Bishop, D. K. (2019). RPA resolves conflicting activities of accessory proteins during reconstitution of Dmc1-mediated meiotic recombination. Nucleic acids research, 47(2), 747-761.

Crickard, J. B., Kaniecki, K., Kwon, Y., Sung, P., & Greene, E. C. (2018). Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments. Journal of Biological Chemistry, 293(11), 4191-4200.

Crickard, J. B., Kaniecki, K., Kwon, Y., Sung, P., & Greene, E. C. (2018). Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase. Proceedings of the National Academy of Sciences, 115(43), E10041-E10048.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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