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Mouse Anti-DMRT1 Recombinant Antibody (10B2752) (CBMAB-D1215-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Doublesex And Mab-3 Related Transcription Factor 1 (DMRT1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DMRT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
10B2752
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-374

Target

Full Name
doublesex and mab-3 related transcription factor 1
Introduction
DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous.
Entrez Gene ID
UniProt ID
Alternative Names
Doublesex And Mab-3 Related Transcription Factor 1; DM Domain Expressed In Testis Protein 1; DM Domain Expressed In Testis 1; DMT1; Doublesex- And Mab-3-Related Transcription Factor 1; CT154;
Function
Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis (By similarity).
Biological Process
Cell morphogenesis Source: Ensembl
Germ cell development Source: GO_Central
Germ cell migration Source: Ensembl
Intracellular signal transduction Source: Ensembl
Male germ cell proliferation Source: UniProtKB
Male sex determination Source: UniProtKB
Male sex differentiation Source: UniProtKB
Negative regulation of meiotic nuclear division Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Oocyte development Source: Ensembl
Positive regulation of male gonad development Source: Ensembl
Positive regulation of meiosis I Source: Ensembl
Positive regulation of mitotic nuclear division Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of nodal signaling pathway Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Sertoli cell development Source: Ensembl
Sertoli cell differentiation Source: UniProtKB
Sex differentiation Source: GO_Central
Spermatogenesis Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Testicular germ cell tumor (TGCT):
A common malignancy in males representing 95% of all testicular neoplasms. TGCTs have various pathologic subtypes including: unclassified intratubular germ cell neoplasia, seminoma (including cases with syncytiotrophoblastic cells), spermatocytic seminoma, embryonal carcinoma, yolk sac tumor, choriocarcinoma, and teratoma.
46,XY sex reversal 4 (SRXY4):
A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients display complete or partial gonadal dysgenesis and a chromosome 9p deletion.

Zarkower, D., & Murphy, M. W. (2021). DMRT1: An Ancient Sexual Regulator Required for Human Gonadogenesis. Sexual Development, 1-14.

Ioannidis, J., Taylor, G., Zhao, D., Liu, L., Idoko-Akoh, A., Gong, D., ... & Clinton, M. (2021). Primary sex determination in birds depends on DMRT1 dosage, but gonadal sex does not determine adult secondary sex characteristics. Proceedings of the National Academy of Sciences, 118(10).

Lindeman, R. E., Murphy, M. W., Agrimson, K. S., Gewiss, R. L., Bardwell, V. J., Gearhart, M. D., & Zarkower, D. (2021). The conserved sex regulator DMRT1 recruits SOX9 in sexual cell fate reprogramming. Nucleic acids research, 49(11), 6144-6164.

Dai, S., Qi, S., Wei, X., Liu, X., Li, Y., Zhou, X., ... & Li, M. (2021). Germline sexual fate is determined by the antagonistic action of dmrt1 and foxl3/foxl2 in tilapia. Development, 148(8).

Cui, Z., Liu, Y., Wang, W., Wang, Q., Zhang, N., Lin, F., ... & Chen, S. (2017). Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis). Scientific reports, 7(1), 1-10.

Lin, Q., Mei, J., Li, Z., Zhang, X., Zhou, L., & Gui, J. F. (2017). Distinct and cooperative roles of amh and dmrt1 in self-renewal and differentiation of male germ cells in zebrafish. Genetics, 207(3), 1007-1022.

Ge, C., Ye, J., Zhang, H., Zhang, Y., Sun, W., Sang, Y., ... & Qian, G. (2017). Dmrt1 induces the male pathway in a turtle species with temperature-dependent sex determination. Development, 144(12), 2222-2233.

Huang, S., Ye, L., & Chen, H. (2017). Sex determination and maintenance: the role of DMRT1 and FOXL2. Asian journal of andrology, 19(6), 619.

Webster, K. A., Schach, U., Ordaz, A., Steinfeld, J. S., Draper, B. W., & Siegfried, K. R. (2017). Dmrt1 is necessary for male sexual development in zebrafish. Developmental biology, 422(1), 33-46.

Sun, W., Cai, H., Zhang, G., Zhang, H., Bao, H., Wang, L., ... & Ge, C. (2017). Dmrt1 is required for primary male sexual differentiation in Chinese soft-shelled turtle Pelodiscus sinensis. Scientific Reports, 7(1), 1-14.

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For research use only. Not intended for any clinical use.

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