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Mouse Anti-DNAJB1 Recombinant Antibody (K1C7) (CBMAB-D1285-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DnaJ Heat Shock Protein Family (Hsp40) Member B1 (DNAJB1). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DNAJB1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
K1C7
Antibody Isotype
IgG2b
Application
ELISA, WB

Basic Information

Immunogen
Recombinant human Hsp40(1-340a.a.) purified from E. coli
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-340

Target

Full Name
DnaJ Heat Shock Protein Family (Hsp40) Member B1
Introduction
DNAJB1 belongs to the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation.
Entrez Gene ID
3337
UniProt ID
P25685
Alternative Names
DnaJ Heat Shock Protein Family (Hsp40) Member B1; DnaJ (Hsp40) Homolog, Subfamily B, Member 1; Heat Shock 40 KDa Protein 1; DnaJ Protein Homolog 1; Human DnaJ Protein 1; Hsp40; HSPF1; Hdj1; Radial Spoke 16 Homolog B (Chlamydomonas);
Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401).

Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
Biological Process
Chaperone cofactor-dependent protein refolding Source: UniProtKB
Forebrain development Source: Ensembl
Negative regulation of inclusion body assembly Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: GO_Central
Negative regulation of transcription from RNA polymerase II promoter in response to stress Source: UniProtKB
Positive regulation of ATPase activity Source: BHF-UCL
Regulation of cellular response to heat Source: Reactome
Response to unfolded protein Source: ProtInc
Cellular Location
Nucleus; Nucleolus; Cytoplasm. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

Zhang, Z., Zhang, X., Wu, X., Zhang, Y., Lu, J., & Li, D. (2022). Sirt1 Attenuates Astrocyte Activation Via Modulating Dnajb1 and Chaperone-Mediated Autophagy after Closed Head Injury. Cerebral Cortex.

Kim, S. S., Kycia, I., Karski, M., Ma, R. K., Bordt, E. A., Kwan, J., ... & Vakili, K. (2022). DNAJB1-PRKACA in HEK293T cells induces LINC00473 overexpression that depends on PKA signaling. PloS one, 17(2), e0263829.

Vyas, M., Hechtman, J. F., Zhang, Y., Benayed, R., Yavas, A., Askan, G., ... & Basturk, O. (2020). DNAJB1-PRKACA fusions occur in oncocytic pancreatic and biliary neoplasms and are not specific for fibrolamellar hepatocellular carcinoma. Modern Pathology, 33(4), 648-656.

Lu, T. W., Aoto, P. C., Weng, J. H., Nielsen, C., Cash, J. N., Hall, J., ... & Taylor, S. S. (2020). Structural analyses of the PKA RIIβ holoenzyme containing the oncogenic DnaJB1-PKAc fusion protein reveal protomer asymmetry and fusion-induced allosteric perturbations in fibrolamellar hepatocellular carcinoma. PLoS biology, 18(12), e3001018.

Tomasini, M. D., Wang, Y., Karamafrooz, A., Li, G., Beuming, T., Gao, J., ... & Simon, S. M. (2018). Conformational landscape of the PRKACA-DNAJB1 chimeric kinase, the driver for fibrolamellar hepatocellular carcinoma. Scientific reports, 8(1), 1-11.

Silva, G., Marins, M., Chaichanasak, N., Yoon, Y., Fachin, A. L., Pinhanelli, V. C., ... & Baek, S. J. (2018). Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1 inhibition. PLoS One, 13(8), e0202263.

Graham, R. P., Lackner, C., Terracciano, L., González‐Cantú, Y., Maleszewski, J. J., Greipp, P. T., ... & Torbenson, M. S. (2018). Fibrolamellar carcinoma in the Carney complex: PRKAR1A loss instead of the classic DNAJB1‐PRKACA fusion. Hepatology, 68(4), 1441-1447.

Engelholm, L. H., Riaz, A., Serra, D., Dagnæs-Hansen, F., Johansen, J. V., Santoni-Rugiu, E., ... & Frödin, M. (2017). CRISPR/Cas9 engineering of adult mouse liver demonstrates that the Dnajb1–Prkaca gene fusion is sufficient to induce tumors resembling fibrolamellar hepatocellular carcinoma. Gastroenterology, 153(6), 1662-1673.

Kastenhuber, E. R., Lalazar, G., Houlihan, S. L., Tschaharganeh, D. F., Baslan, T., Chen, C. C., ... & Lowe, S. W. (2017). DNAJB1–PRKACA fusion kinase interacts with β-catenin and the liver regenerative response to drive fibrolamellar hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 114(50), 13076-13084.

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For research use only. Not intended for any clinical use.

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