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Rabbit Anti-DPH1 Recombinant Antibody (N-A) (CBMAB-D1549-YC)

Provided herein is a Rabbit monoclonal antibody, which binds to Diphthamide Biosynthesis 1 (DPH1). The antibody can be used for immunoassay techniques, such as WB.
See all DPH1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
N-A
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Synthetic peptide: RGRISRGRLANQ conjugated to KLH, corresponding to AA 20-31 of Human OVCA1
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DPH1 homolog (S. cerevisiae)
Introduction
DPH1 is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
Entrez Gene ID
1801
UniProt ID
Q9BZG8
Alternative Names
Diphthamide Biosynthesis 1; S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 1; Ovarian Cancer-Associated Gene 1 Protein; Diphtheria Toxin Resistance Protein 1; Ovarian Tumor Suppressor Candidate 1; Diphthamide Biosynthesis Protein 1; DPH2L1; DPH2L; OVCA1; Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 1 (S. Cerevisiae); Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis (Saccharomyces)-Like 1;
Function
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity).

Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity).
Biological Process
Peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central
Cellular Location
Cytoplasm; Nucleus. Punctate, primarily perinuclear localization.
Involvement in disease
Developmental delay with short stature, dysmorphic facial features, and sparse hair (DEDSSH):
An autosomal recessive syndrome characterized by intellectual disability, short stature, and craniofacial and ectodermal anomalies including scaphocephaly with or without craniosynostosis, prominent forehead, sparse eyebrows and hair, hypoplastic toenails and, in some cases, dental anomalies.

Zhang, Y., Su, D., Dzikovski, B., Majer, S. H., Coleman, R., Chandrasekaran, S., ... & Lin, H. (2021). Dph3 Enables Aerobic Diphthamide Biosynthesis by Donating One Iron Atom to Transform a [3Fe–4S] to a [4Fe–4S] Cluster in Dph1–Dph2. Journal of the American Chemical Society, 143(25), 9314-9319.

Liu, J., Zuo, Z., Zou, M., Finkel, T., & Liu, S. (2020). Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen. PLoS genetics, 16(10), e1009068.

Dong, M., Dando, E. E., Kotliar, I., Su, X., Dzikovski, B., Freed, J. H., & Lin, H. (2019). The asymmetric function of Dph1–Dph2 heterodimer in diphthamide biosynthesis. JBIC Journal of Biological Inorganic Chemistry, 24(6), 777-782.

Dong, M., Kathiresan, V., Fenwick, M. K., Torelli, A. T., Zhang, Y., Caranto, J. D., ... & Lin, H. (2018). Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science, 359(6381), 1247-1250.

Sekiguchi, F., Nasiri, J., Sedghi, M., Salehi, M., Hosseinzadeh, M., Okamoto, N., ... & Matsumoto, N. (2018). A novel homozygous DPH1 mutation causes intellectual disability and unique craniofacial features. Journal of Human Genetics, 63(4), 487-491.

Stephansky, J., Togami, K., Ghandi, M., Montero, J., Lindsay, R., Brooks, C., ... & Lane, A. A. (2017). Resistance to SL-401 in AML and BPDCN is associated with loss of the diphthamide synthesis pathway enzyme DPH1 and is reversible by azacitidine. Blood, 130, 797.

Mayer, K., Schröder, A., Schnitger, J., Stahl, S., & Brinkmann, U. (2017). Influence of DPH1 and DPH5 protein variants on the synthesis of diphthamide, the target of ADPRibosylating toxins. Toxins, 9(3), 78.

Liu, M., Yin, K., Guo, X., Feng, H., Yuan, M., Liu, Y., ... & Chen, X. (2017). Diphthamide biosynthesis 1 is a novel oncogene in colorectal cancer cells and is regulated by MiR-218-5p. Cellular Physiology and Biochemistry, 44(2), 505-514.

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For research use only. Not intended for any clinical use.

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