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Mouse Anti-E. coli dam Recombinant Antibody (RB1) (CBMAB-V208-1692-CQ)

This product is a mouse antibody that recognizes E. coli dam. The antibody RB1 can be used for immunoassay techniques such as: WB.
See all E. coli dam antibodies

Summary

Host Animal
Mouse
Specificity
Bacteria
Clone
RB1
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Recombinant DNA Adenine Methylase protein
Specificity
Bacteria
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.08% sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA adenine methyltransferase
Introduction
Dam is a base flipping enzyme. DNA adenine methyltransferase (Dam), which is responsible for methylation of nearly all GATC sequences in Escherichia coli, is encoded by the dam gene and occurs at about 130 molecules per rapidly growing cell.
Entrez Gene ID
UniProt ID
Alternative Names
dam; DNA adenine methyltransferase; ECK3374;
Research Area
Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
Biological Process
Bacterial-type DNA replication initiation Source: EcoCyc
DNA-dependent DNA replication Source: EcoliWiki
DNA methylation on adenine Source: EcoCyc
Mismatch repair Source: EcoliWiki
Response to UV Source: EcoCyc
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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