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Recombinant Mouse Anti-Ebola Virus Viral Protein 40(VP 40) Antibody (CB3158) (CBMAB-MD1259-LY)

The product is antibody recognizes Ebola Virus. The antibody CB3158 immunoassay techniques such as: ELISA, WB.
See all Ebola Virus Viral Protein 40(VP 40) antibodies

Summary

Host Animal
Mouse
Specificity
Ebola Virus
Clone
CB3158
Antibody Isotype
IgG2b
Application
ELISA, WB

Basic Information

Immunogen
Recombinant Ebola Virus VP40
Specificity
Ebola Virus
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.1% Sodium Azide
Purity
> 90% Purity determined by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ebola Virus Viral Protein 40(VP 40)
Research Area
Plays an essential role virus particle assembly and budding (PubMed:16719918, PubMed:33673144).

Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101 (PubMed:15892969, PubMed:16719918, PubMed:23637409, PubMed:25786915, PubMed:26753796, PubMed:27489272).

The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding (PubMed:33673144).

May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis) (PubMed:27872619).
Biological Process
Intracellular transport of virus Source: CACAO
Mitigation of host immune response by virus Source: UniProtKB-KW
Suppression of host defenses by symbiont Source: CACAO
Viral budding Source: CACAO
Viral budding from plasma membrane Source: UniProtKB
Viral budding via host ESCRT complex Source: UniProtKB-KW
Cellular Location
Host cytoplasm; Host cell membrane; Virion membrane; Host late endosome membrane; Host cell membrane; Host endomembrane system; Extracellular exosome. In virion, localizes on the inner side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place.
PTM
Sumoylated with host SUMO1, SUMO2. Sumoylation provides stability to VP40 (PubMed:27849047). Acetylated by host EP300 in vitro (PubMed:30205953).
Ubiquitinated by host WWP1. This modification mediates efficient viral budding.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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