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Mouse Anti-EIF2S3 Recombinant Antibody (CBFYE-0671) (CBMAB-E1088-FY)

This product is mouse antibody that recognizes EIF2S3. The antibody CBFYE-0671 can be used for immunoassay techniques such as: ELISA, IHC-P, WB.
See all EIF2S3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0671
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Introduction
The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit.
Entrez Gene ID
UniProt ID
Alternative Names
EIF2; EIF2G; MEHMO; MRXSBRK; eIF-2gA; EIF2gamma
Research Area
As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B (By similarity).

Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (By similarity).
Biological Process
Formation of translation preinitiation complex Source: GO_Central
Positive regulation of translational fidelity Source: GO_Central
Translational initiation Source: UniProtKB
Cellular Location
Cytosol; Extracellular exosome; Cytoplasm; Eukaryotic translation initiation factor 2 complex
Involvement in disease
MEHMO syndrome (MEHMO):
An X-linked recessive mental retardation syndrome characterized by intellectual disability, epileptic seizures, hypogonadism and hypogenitalism, microcephaly, and obesity.

Ivanova, N., Serzhanova, V., Demina, N., Guseva, D., & Skoblov, M. (2022). mRNA analysis revealed a novel pathogenic EIF2S3 variant causing MEHMO syndrome. European Journal of Medical Genetics, 65(2), 104421.

Moortgat, S., Manfroid, I., Pendeville, H., Freeman, S., Bourdouxhe, J., Benoit, V., ... & Maystadt, I. (2021). Broadening the phenotypic spectrum and physiological insights related to EIF2S3 variants. Human Mutation, 42(7), 827-834.

Söylemez, Z., Arikan, E. S., Solak, M., Arikan, Y., Tokyol, Ç., & Şeker, H. (2021). Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors. Turkish Journal of Medical Sciences, 51(2), 661-674.

Kotzaeridou, U., Young‐Baird, S. K., Suckow, V., Thornburg, A. G., Wagner, M., Harting, I., ... & Kalscheuer, V. M. (2020). Novel pathogenic EIF2S3 missense variants causing clinically variable MEHMO syndrome with impaired eIF2γ translational function, and literature review. Clinical genetics, 98(5), 507-514.

Rohozinski, J., & Edwards, C. L. (2020). Does EIF2S3 Retrogene Activation Regulate Cancer/Testis Antigen Expression in Human Cancers?. Frontiers in Oncology, 10, 590408.

SÖylemez, Z., Arikan, E. S., Solak, M., Arikan, Y., Tokyol, Ç., & Şeker, H. (2020). Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A, IFNgamma, PIK3CA and CTNNB1 genes in different stage colorectal tumors. Turkish Journal of Medical Sciences.

Gregory, L. C., Ferreira, C. B., Young-Baird, S. K., Williams, H. J., Harakalova, M., van Haaften, G., ... & Dattani, M. T. (2019). Impaired EIF2S3 function associated with a novel phenotype of X-linked hypopituitarism with glucose dysregulation. EBioMedicine, 42, 470-480.

Young-Baird, S. K., Shin, B. S., & Dever, T. E. (2019). MEHMO syndrome mutation EIF2S3-I259M impairs initiator Met-tRNAiMet binding to eukaryotic translation initiation factor eIF2. Nucleic Acids Research, 47(2), 855-867.

Stanik, J., Skopkova, M., Stanikova, D., Brennerova, K., Barak, L., Ticha, L., ... & Gasperikova, D. (2018). Neonatal hypoglycemia, early-onset diabetes and hypopituitarism due to the mutation in EIF2S3 gene causing MEHMO syndrome. Physiol Res, 67(2), 331-337.

Lu, J., Xu, L., Luo, X., Chen, S., Zou, Y., & Tan, H. (2018). The Expression of EIF2S3 in Acute Myeloid Leukemia and Its Prognostic Significance.

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For research use only. Not intended for any clinical use.

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