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Mouse Anti-EIF4E (AA 1-217) Recombinant Antibody (CBFYE-0121) (CBMAB-E0243-FY)

This product is mouse antibody that recognizes EIF4E. The antibody CBFYE-0121 can be used for immunoassay techniques such as: WB.
See all EIF4E antibodies

Summary

Host Animal
Mouse
Specificity
Human, Chicken, Dog, Frog, Mouse, Rat
Clone
CBFYE-0121
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Rabbit eIF-4E aa. 1-217
Specificity
Human, Chicken, Dog, Frog, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.25 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-217

Target

Full Name
Eukaryotic Translation Initiation Factor 4E
Introduction
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
Human1977
Mouse13684
Rat117045
Chicken422703
Dog487870
Frog548663
UniProt ID
HumanP06730
MouseP63073
RatP63074
ChickenA0A3Q2TU97
DogF1PSC3
FrogP48597
Alternative Names
Eukaryotic Translation Initiation Factor 4E; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF4EL1; EIF-4E; EIF4F
Research Area
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:16271312, PubMed:22578813).

In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm (PubMed:24335285).

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap (By similarity).

Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).

In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).

May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).
Biological Process
Behavioral fear response Source: Ensembl
Cellular response to dexamethasone stimulus Source: Ensembl
G1/S transition of mitotic cell cycle Source: UniProtKB
Lung development Source: Ensembl
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: Ensembl
Positive regulation of mitotic cell cycle Source: UniProtKB
Regulation of translation Source: UniProtKB
Regulation of translation at postsynapse, modulating synaptic transmission Source: Ensembl
Stem cell population maintenance Source: Ensembl
Cellular Location
Nucleus; Cytoplasm; P-body; Stress granule. Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257).
Involvement in disease
Autism 19 (AUTS19):
Disease susceptibility is associated with variants affecting the gene represented in this entry. A heterozygous single-nucleotide insertion has been found in families affected by autism. The variant results in increased promoter activity and is involved in disease pathogenesis through EIF4E deregulation (PubMed:19556253). A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. A chromosomal aberration involving EIF4E has been found in a patient with classic autism. Translocation t(45)(q23q31.3). The breakpoint on chromosome 4 is located 56 kb downstream of EIF4E (PubMed:19556253).
PTM
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Krause, L., Willing, F., Andreou, A. Z., & Klostermeier, D. (2022). The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects. Nucleic acids research, 50(11), 6497-6510.

Conn, C. S., Yang, H., Tom, H. J., Ikeda, K., Oses-Prieto, J. A., Vu, H., ... & Ruggero, D. (2021). The major cap-binding protein eIF4E regulates lipid homeostasis and diet-induced obesity. Nature Metabolism, 3(2), 244-257.

Romagnoli, A., D’Agostino, M., Ardiccioni, C., Maracci, C., Motta, S., La Teana, A., & Di Marino, D. (2021). Control of the eIF4E activity: structural insights and pharmacological implications. Cellular and Molecular Life Sciences, 78(21), 6869-6885.

Aguilar-Valles, A., De Gregorio, D., Matta-Camacho, E., Eslamizade, M. J., Khlaifia, A., Skaleka, A., ... & Sonenberg, N. (2021). Antidepressant actions of ketamine engage cell-specific translation via eIF4E. Nature, 590(7845), 315-319.

Knight, J. R., Alexandrou, C., Skalka, G. L., Vlahov, N., Pennel, K., Officer, L., ... & Sansom, O. J. (2021). MNK Inhibition Sensitizes KRAS-Mutant Colorectal Cancer to mTORC1 Inhibition by Reducing eIF4E Phosphorylation and c-MYC ExpressionTargeting P-eIF4E and mTORC1 in KRAS-Mutant Colorectal Cancer. Cancer discovery, 11(5), 1228-1247.

Yang, X., Zhong, W., & Cao, R. (2020). Phosphorylation of the mRNA cap-binding protein eIF4E and cancer. Cellular signalling, 73, 109689.

Wan, X., Yang, T., Cuesta, A., Pang, X., Balius, T. E., Irwin, J. J., ... & Taunton, J. (2020). Discovery of lysine-targeted eIF4E inhibitors through covalent docking. Journal of the American Chemical Society, 142(11), 4960-4964.

Culjkovic-Kraljacic, B., Skrabanek, L., Revuelta, M. V., Gasiorek, J., Cowling, V. H., Cerchietti, L., & Borden, K. L. (2020). The eukaryotic translation initiation factor eIF4E elevates steady-state m7G capping of coding and noncoding transcripts. Proceedings of the National Academy of Sciences, 117(43), 26773-26783.

Coutinho de Oliveira, L., Volpon, L., Rahardjo, A. K., Osborne, M. J., Culjkovic-Kraljacic, B., Trahan, C., ... & Borden, K. L. (2019). Structural studies of the eIF4E–VPg complex reveal a direct competition for capped RNA: Implications for translation. Proceedings of the National Academy of Sciences, 116(48), 24056-24065.

Amorim, I. S., Lach, G., & Gkogkas, C. G. (2018). The role of the eukaryotic translation initiation factor 4E (eIF4E) in neuropsychiatric disorders. Frontiers in genetics, 9, 561.

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For research use only. Not intended for any clinical use.

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