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Rat Anti-ESAM Recombinant Antibody (CBFYE-1219) (V2LY-0825-LY750)

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Published Data
V2LY-0825-LY750-1
Rat Anti-ESAM Recombinant Antibody (CBFYE-1219) (V2LY-0825-LY750) in IHC
ESAM histochemical staining in tissue samples.

ESAM staining is shown in lung tumor (A,C) and normal lung (B,D) distant from the tumor. Endothelial cells are visibly more abundant in the normal lung section, consistent with the high vascularity of normal lung. Raw images are shown in A and C, with ESAM-positive cells identified by the CellProfiler algorithm marked with a "1" in images B and D. ...View More
Mehan, M. R., Ayers, D., Thirstrup, D., Xiong, W., Ostroff, R. M., Brody, E. N., ... & Wilcox, S. K. (2012). Protein signature of lung cancer tissues. PloS one, 7(4), e35157.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rat
Clone
CBFYE-1219
Application
IHC
Immunogen
Recombinant mouse ESAM (Gln30-Ser248).
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC8-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, trehalose
Preservative
None
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
endothelial cell adhesion molecule
Entrez Gene ID
69524
UniProt ID
Q925F2
Research Area
Can mediate aggregation most likely through a homophilic molecular interaction.
Biological Process
Bicellular tight junction assembly Source: ARUK-UCL
Cell-cell adhesion Source: ARUK-UCL
Cellular protein localization Source: ARUK-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: Ensembl
Maintenance of blood-brain barrier Source: ARUK-UCL
Regulation of actin cytoskeleton reorganization Source: ARUK-UCL
Regulation of actin filament polymerization Source: ARUK-UCL
Cellular Location
Cell membrane; Adherens junction; Tight junction
Topology
Extracellular: 30-248
Helical: 249-269
Cytoplasmic: 270-390

Hansmeier, N. R., Büschlen, I. S., Behncke, R. Y., Ulferts, S., Bisoendial, R., & Hägerling, R. (2022). 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM). International journal of molecular sciences, 23(8), 4369.

Buncha, V., Fopiano, K. A., Tian, Y., & Bagi, Z. (2021). Role of Endothelial Cell Specific Adhesion Molecule in the Development of Pulmonary Microvascular Dysfunction in HFpEF. The FASEB Journal, 35.

Duong, C. N., Nottebaum, A. F., Butz, S., Volkery, S., Zeuschner, D., Stehling, M., & Vestweber, D. (2020). Interference with ESAM (endothelial cell-selective adhesion molecule) plus vascular endothelial-cadherin causes immediate lethality and lung-specific blood coagulation. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(2), 378-393.

Ueda, T., Yokota, T., Uno, Y., Mashimo, T., Sudo, T., Ishibashi, T., ... & Kanakura, Y. (2018). ESAM on the Cells of Endothelial Lineage Plays an Important Role in the Development of Definitive Hematopoiesis. Blood, 132, 3848.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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