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Mouse Anti-EXOSC10 Recombinant Antibody (1E6) (CBMAB-A2753-LY)

The product is antibody recognizes EXOSC10. The antibody 1E6 immunoassay techniques such as: WB, ELISA.
See all EXOSC10 antibodies
Published Data
Fig. 1 HeLa cells were grown on cover slips, fixed, and stained according to the protocol in Kedersha and Anderson with the indicated antibodies.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1E6
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
EXOSC10 (NP_001001998.1, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
exosome component 10
Entrez Gene ID
5394
UniProt ID
Q01780
Alternative Names
PM-Scl; PM/Scl-100; PMSCL; PMSCL2; RRP6; Rrp6p; p2; p3; p4
Research Area
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity).

EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPHOSPH6 with the RNA exosome involved in the maturation of 5.8S rRNA.
Biological Process
CUT catabolic process Source: UniProtKB
Dosage compensation by inactivation of X chromosome Source: Ensembl
Exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
Histone mRNA catabolic process Source: UniProtKB
Maturation of 5.8S rRNA Source: UniProtKB
Negative regulation of telomere maintenance via telomerase Source: BHF-UCL
Nuclear mRNA surveillance Source: UniProtKB
Nuclear polyadenylation-dependent antisense transcript catabolic process Source: GO_Central
Nuclear polyadenylation-dependent CUT catabolic process Source: GO_Central
Nuclear polyadenylation-dependent rRNA catabolic process Source: UniProtKB
Nuclear polyadenylation-dependent snoRNA catabolic process Source: GO_Central
Nuclear polyadenylation-dependent snRNA catabolic process Source: GO_Central
Nuclear polyadenylation-dependent tRNA catabolic process Source: GO_Central
Nuclear retention of unspliced pre-mRNA at the site of transcription Source: UniProtKB
Nuclear-transcribed mRNA catabolic process Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Polyadenylation-dependent snoRNA 3'-end processing Source: GO_Central
Regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
rRNA processing Source: Reactome
Cellular Location
Nucleolus; Nucleus; Cytoplasm. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.

Petit, F. G., Jamin, S. P., Kernanec, P. Y., Becker, E., Halet, G., & Primig, M. (2022). EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition. Developmental Biology, 483, 58-65.

Stuparević, I., Novačić, A., Rahmouni, A. R., Fernandez, A., Lamb, N., & Primig, M. (2021). Regulation of the conserved 3′‐5′ exoribonuclease EXOSC10/Rrp6 during cell division, development and cancer. Biological Reviews, 96(4), 1092-1113.

Stuparevic, I., Novacic, A., Oskomic, M., Strbac, L., Beauvais, V., Primig, M., & Rahmouni, A. R. (2021). Roles of Rrp6/EXOSC10-targeted lncRNAs in anti-cancer drug toxicity and cell wall architecture. FEBS Open Bio, 11, 102-102.

Wu, D., & Dean, J. (2020). EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes. Nucleic acids research, 48(10), 5349-5365.

Oreški, L. (2019). Analiza ekspresije exosc10/Exosc10 u tkivima zebrice (Danio rerio) i embrionalnim fazama razvoja (Doctoral dissertation, University of Zagreb. Faculty of Food Technology and Biotechnology. Department of Chemistry and Biochemistry. Laboratory for Biochemistry).

Davidson, L., Francis, L., Cordiner, R. A., Eaton, J. D., Estell, C., Macias, S., ... & West, S. (2019). Rapid depletion of DIS3, EXOSC10, or XRN2 reveals the immediate impact of exoribonucleolysis on nuclear RNA metabolism and transcriptional control. Cell reports, 26(10), 2779-2791.

Domingo-Prim, J., Endara-Coll, M., Bonath, F., Jimeno, S., Prados-Carvajal, R., Friedländer, M. R., ... & Visa, N. (2019). EXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks. Nature communications, 10(1), 1-13.

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For research use only. Not intended for any clinical use.

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